Quantification of β ‐eudesmol in rat plasma using LC–MS/MS and its application to a pharmacokinetic study

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EarlyView Article

  • Published: Jul 18, 2017
  • Author: Ligang Jiang, Chunyang Zhang, Haiping Li
  • Journal: Biomedical Chromatography

Abstract

A sensitive and specific LC–MS/MS assay for determination of β‐eudesmol in rat plasma was developed and validated. After liquid–liquid extraction with ethyl ether, the analyte and IS were separated on a Capcell Pak C18 column (50 × 2.0 mm, 5 μm) by isocratic elution with acetonitrile—water–formic acid (77.5:22.5:0.1, v/v/v) as the mobile phase at a flow rate of 0.4 mL/min. An ESI source was applied and operated in positive ion mode; a selected reaction monitoring scan was used for quantification by monitoring the precursor–product ion transitions of m/z 245.1 → 163.1 for β‐eudesmol and m/z 273.4 → 81.2 for IS. Good linearity was observed in the concentration range of 3–900 ng/mL for β‐eudesmol in rat plasma. Intra‐ and inter‐day precision and accuracy were both within ±14.3%. This method was applied for pharmacokinetic studies after intravenous bolus of 2.0 mg/kg or intragastric administration of 50 mg/kg β‐eudesmol in rats.

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