Non‐targeted acquisition strategy for screening doping compounds based on GC‐EI‐hybrid quadrupole‐Orbitrap mass spectrometry: A focus on exogenous anabolic steroids

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EarlyView Article

  • Published: Jul 27, 2017
  • Author: Gustavo Albuquerque Cavalcanti, Lucas Martins Rodrigues, Leonardo Santos, Xin Zheng, Amit Gujar, Jason Cole, Monica Costa Padilha, Francisco Radler Aquino Neto
  • Journal: Drug Testing and Analysis

This is a first look at a non‐targeted screening method based on Orbitrap gas chromatography–mass spectrometry (GC–MS) technology for a large number of banned compounds in sports found in urine, including exogenous anabolic steroids, β‐agonists, narcotics, stimulants, hormone modulators, and diuretics. A simple sample preparation was processed in four steps: an enzymatic hydrolysis, liquid–liquid extraction, evaporation, and trimethylsilylation. All compounds were able to meet the World Anti‐Doping Agency's sensitivity criteria with mass accuracies less than 1 ppm and with sufficient points across the peak by running the Orbitrap GC–MS in full‐scan mode. In addition, we discuss our initial findings of using a full‐scan selected ion monitoring‐tandem mass spectrometry (SIM‐MS/MS) approach as a way to obtain lower detection limits and reach desirable selectivity for some exogenous anabolic steroids.

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