Detection of Sotatercept (ACE‐011) in human serum by SAR‐PAGE and western single blotting

Skip to Navigation

EarlyView Article

  • Published: Dec 27, 2017
  • Author: Christian Reichel, Letizia Farmer, Günter Gmeiner, Katja Walpurgis, Mario Thevis
  • Journal: Drug Testing and Analysis


A method for the detection of Sotatercept (ACE‐011, ACVR2A‐Fc) in human serum is presented. The method is a modification of a recently published protocol for Luspatercept (ACE‐536, ACVR2B‐Fc), another erythropoiesis stimulating fusion protein. Out of 27 tested antibodies against either the extracellular domain of ACVR2A or the full‐length protein, only 4 antibodies bound strongly enough to Sotatercept for usage with immunoprecipitation followed by SAR‐PAGE and Western single blotting. The adapted protocol allows the detection of 0.1 ng/mL Sotatercept in just 50 μL human serum. None of the 3 commercial ACVR2‐ELISAs was able to detect Sotatercept and the 2 tested surrogate proteins, even in the μg/mL range. As for Luspatercept, only IPG‐IEF/2D‐PAGE generated discrete isoforms. Due to the long serum half‐life, the SAR‐PAGE method will be able to detect Sotatercept for several weeks and will be very useful in doping testing.

Social Links

Share This Links

Bookmark and Share


Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site

Copyright © 2018 John Wiley & Sons, Inc. All Rights Reserved