Immobilized monolithic enzymatic reactor and its application for analysis of in‐vitro fertilization media samples

Skip to Navigation

EarlyView Article

  • Published: Jul 10, 2017
  • Author: Wei‐Qiang Chen, Philipp Obermayr, Urh Černigoj, Jana Vidič, Tanta Panić‐Janković, Goran Mitulović

Classical proteomics approaches involve enzymatic hydrolysis of proteins (either separated by polyacrylamide gels or in solution) followed by peptide identification using LC‐MS/MS analysis. This method requires normally more than 16 h to complete. In the case of clinical analysis, it is of the utmost importance to provide fast and reproducible analysis with minimal manual sample handling. Herein we report the method development for online protein digestion on immobilized monolithic enzymatic reactors (IMER) to accelerate protein digestion, reduce manual sample handling, and provide reproducibility to the digestion process in clinical laboratory. An integrated online digestion and separation method using monolithic immobilized enzymatic reactor was developed and applied to digestion and separation of in‐vitro‐fertilization media.

Social Links

Share This Links

Bookmark and Share


Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved