Preparation and characterization of a Cu (II)‐IDA poly HEMA monolith syringe for proteomic applications

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EarlyView Article

  • Published: Aug 28, 2017
  • Author: Ashish Khaparde, Vijayalakshmi M. A., Kishore K. R. Tetala
  • Journal: ELECTROPHORESIS

Herein, we report the preparation of a metal‐chelate immobilized hydrophilic poly hydroxyethylmethacrylate‐based monolith of 100 μL volume in a plastic syringe. The monolith is elastic in nature, contains well interconnected pores with a permeability (k) of 1.3 × 10−12 m2. Immobilization of iminodiacetic acid (IDA) is performed via schiff base reaction. Adsortion of IgG on this copper‐IDA monolith is of langmuir isotherm with a maximum adsorption capacity of ∼25 mg IgG per g monolith. IgG adsorption capacity of this affinity monolith remained unaffected with increase of flow rate. This proposed metal‐chelate monolith in syringe format has the potential for application in proteomics.

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