SERS analysis of selectively captured exosomes using an integrin‐specific peptide ligand

Skip to Navigation

EarlyView Article

  • Published: Aug 29, 2017
  • Author: Changwon Lee, Randy Carney, Kit Lam, James W. Chan
  • Journal: Journal of Raman Spectroscopy

Abstract

We demonstrate selective capturing of exosomes for surface‐enhanced Raman spectroscopy (SERS) analysis using a thiolated peptide ligand that is bound to the surface of silver nanoparticles via thiol‐metallic bond formation. The ligand preferentially binds to α3ß1 integrin over‐expressed in exosomes secreted from SKOV‐3 cancer cells. Several Raman peaks at 894 cm−1, 1,080 cm−1, 1,252 cm−1, 1,340 cm−1, and 1,380 cm−1 specific to SKOV‐3 exosomes are detected above the background signals generated from the nanoparticle and the ligand. In comparison, α3ß1 integrin negative exosomes secreted from Jurkat cells showed no detectable exosome specific SERS signal, confirming the high specificity of this ligand‐based SERS detection approach. This study shows the potential of developing a target‐specific SERS detection approach for characterizing exosomes from a heterogeneous mixture in body fluid for early‐stage disease diagnostics.

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved