Simultaneous isolation of seven compounds from Glehnia littoralis roots by off‐line overpressured layer chromatography guided by a TLC antioxidant autographic assay

A combinative method using overpressured layer chromatography (OPLC) and TLC bioautography against O₂^•− was developed to separate antioxidants from Glehnia littoralis roots. Through target‐directed isolation by the TLC bioautographic method, seven compounds including five antioxidants were rapidly isolated by OPLC and identified as 1‐linoloyl‐3‐palmitoylglycerol, facarindiol, panaxynol, isoimperatorin, β‐sitosterol, scopoletin, and umbelliferone from G. littoralis roots. This OPLC method is a very suitable separation technique for light‐sensitive polyacetylenes (panaxynol and facarindiol) with higher isolated yields compared to conventional open column chromatography. This is the first report on the separation of polyacetylenes by OPLC.