High‐performance liquid chromatography thermodynamic study of new potential antiepileptic compounds on a cholesterol column using isocratic elution with methanol/water and acetonitrile/water eluent systems

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EarlyView Article

  • Published: Oct 4, 2017
  • Author: Jolanta Flieger, Hanna Trębacz, Magdalena Pizoń, Anna Kowalska, Agnieszka Szczęsna, Tomasz Plech
  • Journal: Journal of Separation Science


Basic thermodynamic functions responsible for retention of new 1,2,4‐triazole derivatives exhibiting varied antiepileptic activity on cholesterol‐based stationary phase were determined. Evaluation of the Gibbs energy change, the change in enthalpy and the change in entropy was based on the van't Hoff relationship representing lnk versus 1/T. A detailed discussion of the van't Hoff equation, exploring the influence of the phase ratio on deviations from linearity in a van't Hoff plot is presented. We show chromatographic evidence to the question of how a varied mobile phase composition may cause different thermodynamic phase ratios. The analysis of data from a differential scanning calorimetry excluded any phase transitions of either the individual solutes or cholesterol stationary phase suspended in the mobile phase components within the studied temperature range.

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