Surfaceomics and surface‐enhanced R aman spectroscopy of environmental microbes: Matching cofactors with redox‐active surface proteins

Trypsin shaving is a targeted proteomic method for identifying cell‐surface exposed proteins on bacterial cells. For the identification of redox‐active cell‐surface proteins, trypsin‐shaving datasets can be matched with surface‐enhanced Raman spectra of intact cells to identify the cofactors associated with the cell‐surface proteins. Together, these approaches could help resolve questions about the presence of cell‐surface electron transport components in environmental microorganisms, especially microbes that oxidize and reduce metals and metalloids as electron donors and acceptors.