Journal Highlight: Real-time noninvasive monitoring of in vivo inflammatory responses using a pH ratiometric fluorescence imaging probe

Skip to Navigation

Ezine

  • Published: Mar 10, 2014
  • Author: spectroscopyNOW
  • Channels: UV/Vis Spectroscopy
thumbnail image: Journal Highlight: Real-time noninvasive monitoring of in vivo inflammatory responses using a pH ratiometric fluorescence imaging probe
A new ratiometric optical pH probe has been developed by combining pH-sensitive and pH-insensitive fluorescent dyes into nanoparticles for in vivo optical imaging of human inflammatory responses.

Real-time noninvasive monitoring of in vivo inflammatory responses using a pH ratiometric fluorescence imaging probe

Advanced Healthcare Materials, 2014, 3, 221-229
Yi-Ting Tsai, Jun Zhou, Hong Weng, Jinhui Shen, Liping Tang, Wen-Jing Hu

Abstract: It is often difficult to continuously monitor and quantify inflammatory responses in vivo. These dynamic responses however are often accompanied by specific pH changes. A new ratiometric optical pH probe is developed by combining pH-sensitive (CypHer5E) and pH-insensitive (Oyster800) fluorescent dyes into nanoparticles for in vivo optical imaging. By taking the ratio of fluorescence intensities at different wavelengths, these nanosized sensors provide excellent measurement capabilities, and unique mapping, of the continuous in vivo pH changes for three different inflammation models. In each model a strong positive correlation is found between ratiometric pH changes and the corresponding inflammatory response measured by histological analyses. These results indicate that ratiometric imaging can provide a noninvasive, rapid, and highly sensitive optical readout for the pH-ratio changes in vivo. Furthermore this technique may be used to monitor the real-time dynamics of inflammatory processes.

  • This paper is free to view for all users registered on spectroscopyNOW.com until the end of April 2014.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved