Journal Highlight: SERS-based competitive immunoassay of poly-γ-D-glutamic acid for highly sensitive detection of anthrax biomarker

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  • Published: Apr 8, 2015
  • Author: spectroscopyNOW
  • Channels: Raman
thumbnail image: Journal Highlight: SERS-based competitive immunoassay of poly-γ-D-glutamic acid for highly sensitive detection of anthrax biomarker
A SERS-based competitive immunoassay platform has been devised for quantitative evaluation of the anthrax marker poly-γ-D-glutamic acid.

Surface-enhanced Raman scattering-based competitive immunoassay of poly-γ-D-glutamic acid for highly sensitive detection of anthrax biomarker

Bulletin of the Korean Chemical Society, 2015, 36, 822-826
Juhui Ko, Kiweon Cha, Jun Ho Jeon, Gi-eun Rhie, Jonghoon Choi and Jaebum Choo

Abstract: Anthrax is a highly acute disease caused by the bacterium Bacillus anthracis. In this article, we report a highly sensitive detection technique for pathogenic B. anthracis, based on the use of a surface-enhanced Raman scattering (SERS)-active magnetic sensor. Gold nanoparticles (GNPs) and magnetic beads were used as SERS nanotags and supporting substrates, respectively. Here, an SERS-based competitive immunoassay platform is described for the quantitative evaluation of the anthrax marker. Poly-γ-D-glutamic acid (PGA) capsules were used as a target biomarker for the highly sensitive detection of B. anthracis because PGA is known to be closely associated with the pathogenesis of B. anthracis infection. The SERS-based competitive assay reveals an extraordinarily high sensitivity for PGA with a limit of detection of ~1.0 ng/mL. This work provides a conceptually new immunoassay prototype for fast and sensitive detection of PGA markers.

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