Journal Highlight: Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy

Skip to Navigation

Ezine

  • Published: Oct 5, 2015
  • Author: spectroscopyNOW
  • Channels: NMR Knowledge Base
thumbnail image: Journal Highlight: Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy
Recent progress in the solid-state NMR spectroscopy of membrane proteins within a cellular membrane has been reviewed, along with requirements for sample preparation.

Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy

Protein Science, 2015, 24, 1333-1346
Leonid S. Brown and Vladimir Ladizhansky

Abstract: Membrane proteins play many critical roles in cells, mediating flow of material and information across cell membranes. They have evolved to perform these functions in the environment of a cell membrane, whose physicochemical properties are often different from those of common cell membrane mimetics used for structure determination. As a result, membrane proteins are difficult to study by traditional methods of structural biology, and they are significantly underrepresented in the protein structure databank. Solid-state Nuclear Magnetic Resonance (SSNMR) has long been considered as an attractive alternative because it allows for studies of membrane proteins in both native-like membranes composed of synthetic lipids and in cell membranes. Over the past decade, SSNMR has been rapidly developing into a major structural method, and a growing number of membrane protein structures obtained by this technique highlights its potential. Here we discuss membrane protein sample requirements, review recent progress in SSNMR methodologies, and describe recent advances in characterizing membrane proteins in the environment of a cellular membrane.

  • This paper is free to view for all users registered on spectroscopyNOW.com until the end of November 2015.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

Follow us on Twitter!

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved