Journal Highlight: Single-scan multidimensional NMR analysis of mixtures at sub-millimolar concentrations by using SABRE hyperpolarization

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  • Published: Nov 30, 2015
  • Author: spectroscopyNOW
  • Channels: NMR Knowledge Base
thumbnail image: Journal Highlight: Single-scan multidimensional NMR analysis of mixtures at sub-millimolar concentrations by using SABRE hyperpolarization
An approach to obtain clean, SABRE-hyperpolarized 2D 1H NMR spectra of mixtures of small molecules at sub-mM concentrations in a single scan uses para-hydrogen and a deuterated co-substrate for hyperpolarization and ultrafast 2D NMR for acquisition.

Single-scan multidimensional NMR analysis of mixtures at sub-millimolar concentrations by using SABRE hyperpolarization

ChemPhysChem, 2015, 16, 3413-3417
Valeria Daniele, François-Xavier Legrand, Patrick Berthault, Jean-Nicolas Dumez and Gaspard Huber

Abstract: Signal amplification by reversible exchange (SABRE) is a promising method to increase the sensitivity of nuclear magnetic resonance (NMR) experiments. However, SABRE-enhanced 1H NMR signals are short lived, and SABRE is often used to record 1D NMR spectra only. When the sample of interest is a complex mixture, this results in severe overlaps for 1H spectra. In addition, the use of a co-substrate, whose signals may obscure the 1H spectra, is currently the most efficient way to lower the detection limit of SABRE experiments. Here, we describe an approach to obtain clean, SABRE-hyperpolarized 2D 1H NMR spectra of mixtures of small molecules at sub-millimolar concentrations in a single scan. The method relies on the use of para-hydrogen together with a deuterated co-substrate for hyperpolarization and ultrafast 2D NMR for acquisition. It is applicable to all substrates that can be polarized with SABRE.

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