Journal Highlight: Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

Skip to Navigation

Ezine

  • Published: Jun 20, 2016
  • Author: spectroscopyNOW
  • Channels: Proteomics
thumbnail image: Journal Highlight: Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors
To predict how oyster larvae might be affected in a future ocean affected by climate change, changes in the proteome of metamorphosing larvae have been studied under multiple stressors.

Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

Global Change Biology, 2016, 22, 2054-2068
Ramadoss Dineshram, Kondethimmanahalli Chandramouli, Ginger Wai Kuen Ko, Huoming Zhang, Pei-Yuan Qian, Timothy Ravasi and Vengatesen Thiyagarajan

Abstract: The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs.

  • This paper is free to view for all users registered on spectroscopyNOW.com until the end of July 2016.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

Follow us on Twitter!

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved