Journal Highlight: Resonance Raman and UV-visible microscopy reveals that conditioning red blood cells with repeated doses of sodium dithionite increases haemoglobin oxygen uptake

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  • Published: May 1, 2017
  • Author: spectroscopyNOW
  • Channels: Raman
thumbnail image: Journal Highlight: Resonance Raman and UV-visible microscopy reveals that conditioning red blood cells with repeated doses of sodium dithionite increases haemoglobin oxygen uptake

Raman confocal microscopy and UV–vis microscopy showed that successive additions of sodium dithionite to suspensions of red blood cells increased the rate and the capacity to uptake oxygen.

Resonance Raman and UV-visible microscopy reveals that conditioning red blood cells with repeated doses of sodium dithionite increases haemoglobin oxygen uptake

ChemistrySelect, 2017, 2, 3342-3346
David Perez–Guaita, Marleen de Veij, Katarzyna M. Marzec, Abdullah R. D. Almohammedi, Don McNaughton, Andrew J. Hudson and Bayden R. Wood

Abstract: Here we report that successive additions of fresh dithionite to a suspension of red blood cells (RBCs) increase the capacity of the cells to uptake oxygen. This effect was not observed when the RBCs were similarly preconditioned using gaseous N2 to induce short episodes of hypoxia. The effect of successive sodium dithionite and N2 gas additions on a population of functional erythrocytes was monitored using Raman confocal microscopy, with 514 nm excitation, and UV–visible microscopy. The results indicate that successive additions of sodium dithionite in a suspension of red blood cells leads to an increase in both the rate and the capacity of the RBCs to uptake oxygen. The sodium dithionite did not cause haemoglobin from lysed RBCs to uptake more oxygen after successive additions and hence this effect was only observed in functional intact RBCs. Experiments performed with polarised Raman spectroscopy suggest that sodium dithionite increases the disorder of Hb in the RBC facilitating oxygen diffusion.

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