Journal Highlight: Glycan fingerprinting via cold-ion infrared spectroscopy

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  • Published: Jul 3, 2017
  • Author: spectroscopyNOW
  • Channels: Infrared Spectroscopy
thumbnail image: Journal Highlight: Glycan fingerprinting via cold-ion infrared spectroscopy

Cold-ion IR spectroscopy of oligosaccharide ions reveals many well-resolved absorption features that are diagnostic for minute structural variations, making it a key technology combined with mass spectrometry to unravel complex glycomes.

Glycan fingerprinting via cold-ion infrared spectroscopy

Angewandte Chemie International Edition, 2017, online
Eike Mucha, Ana Isabel González Flórez, Mateusz Marianski, Daniel A. Thomas, Waldemar Hoffmann, Weston B. Struwe, Heung S. Hahm, Sandy Gewinner, Dr. Wieland Schöllkopf, Peter H. Seeberger, Gert von Helden and Kevin Pagel

Abstract: The diversity of stereochemical isomers present in glycans and glycoconjugates poses a formidable challenge for comprehensive structural analysis. Typically, sophisticated mass spectrometry (MS)-based techniques are used in combination with chromatography or ion-mobility separation. However, coexisting structurally similar isomers often render an unambiguous identification impossible. Other powerful techniques such as gas-phase infrared (IR) spectroscopy have been limited to smaller glycans, since conformational flexibility and thermal activation during the measurement result in poor spectral resolution. This limitation can be overcome by using cold-ion spectroscopy. The vibrational fingerprints of cold oligosaccharide ions exhibit a wealth of well-resolved absorption features that are diagnostic for minute structural variations. The unprecedented resolution of cold-ion spectroscopy coupled with tandem MS may render this the key technology to unravel complex glycomes.

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