Journal Highlight: Development and validation of an ultraviolet–visible spectrophotometric method for determination of phenylethyl resorcinol in new topical nanoemulsions

Skip to Navigation

Ezine

  • Published: Jul 10, 2017
  • Author: spectroscopyNOW
  • Channels: UV/Vis Spectroscopy
thumbnail image: Journal Highlight: Development and validation of an ultraviolet–visible spectrophotometric method for determination of phenylethyl resorcinol in new topical nanoemulsions

A rapid, simple and efficient UV–vis spectrophotometric method was developed for the quantification of phenylethyl resorcinol, a potent skin-lightening agent, incorporated into new topical nanoemulsions, and to validate this method according to ICH guidelines.

Development and validation of an ultraviolet–visible spectrophotometric method for determination of phenylethyl resorcinol in new topical nanoemulsions

International Journal of Cosmetic Science, 2017, 39, 337-343
L. Hong, D. Han, M.-X. Li, P. Zhang and C.-G. Liu

Abstract: A rapid, simple and efficient ultraviolet–visible (UV–vis) spectrophotometric method was developed for the quantification of phenylethyl resorcinol (PR), a potent skin-lightening agent, incorporated into new topical nanoemulsions, and to validate this method according to International Commission for Harmonization (ICH) guidelines. UV–vis spectrophotometric method for quantification of PR in new topical nanoemulsions was developed and validated in terms of specificity, linearity, sensitivity, precision and accuracy. The method was applied to determine PR content (extraction recoveries) in the nanoemulsions and entrapment efficiencies. The calibration curve for PR was linear in the range of 10–60 μg mL−1, with a determination coefficient (r2) which is higher than 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) were 1.55 and 4.69 μg mL−1, respectively. The method was shown to be specific, precise [intraday, interday and reproducibility levels relative standard deviation < 3%] and accurate (between 91.90 ± 1.28% and 104.73 ± 0.60%). The extraction recoveries of PR in nanoemulsions were 97.07%, 96.64% and 103.54% at concentrations of 3, 6 and 9 mg mL−1, respectively. The entrapment efficiencies were nearly 100%, which agrees with the oil core location of PR in nanoemulsions. This developed method was confirmed to be rapid, simple, cost-effective, specific, precise, accurate and suitable for determination of PR content in nanoemulsions and entrapment efficiencies.

  • This paper is free to view for all users registered on spectroscopyNOW.com until the end of September 2017.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

Follow us on Twitter!

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved