Journal Highlight: Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime

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  • Published: Aug 8, 2017
  • Author: spectroscopyNOW
  • Channels: X-ray Spectrometry
thumbnail image: Journal Highlight: Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime

X-ray tomography of single biological cells in a low-dose regime, based on full-field holographic recordings, has been extended to eliminate the bottleneck of phase retrieval and cope with high noise.

Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime

Acta Crystallographica Section A, 2017, 73, 282-292
Martin Krenkel, Mareike Toepperwien, Frauke Alves and Tim Salditt

Abstract: X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.

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