Journal Highlight: Optimization of extraction and analytical protocol for mass spectrometry‐based metabolomics analysis of hepatotoxicity

Skip to Navigation

Ezine

  • Published: Sep 10, 2018
  • Author: spectroscopyNOW
  • Channels: Base Peak
thumbnail image: Journal Highlight: Optimization of extraction and analytical protocol for mass spectrometry‐based metabolomics analysis of hepatotoxicity

An optimised LC/MS‐based metabolomics protocol for extraction and analysis of endogenous metabolites from liver tissue during hepatotoxicity provides high reliability and reproducibility for polar and nonpolar metabolites.

Optimization of extraction and analytical protocol for mass spectrometry‐based metabolomics analysis of hepatotoxicity

Biomedical Chromatography, 2018, e4359 online
Rui Yang, Qi Zhao, Dan‐Dan Hu, Xue‐Rong Xiao, Fei Li

Abstract: Drug‐induced liver injury (DILI) is a clinically leading side‐effect of drugs. In the present study, a liquid chromatography mass spectrometry‐based metabolomics protocol was optimized for extraction and analysis of endogenous metabolites from liver tissue during hepatotoxicity. Different extraction solutions, resuspension solutions, extraction folds and dissolution ways of supernatant were compared by the number of extracted total ions, relative response and relative extraction efficiency of targeted metabolites from liver tissue, respectively. The polar and nonpolar endogenous metabolites associated with liver injury were analyzed by hydrophilic interaction chromatography (HILIC) and reversed‐phase liquid chromatography (RPLC) with UPLC‐QTOFMS. The results indicated that the extraction with 10‐fold 50% acetonitrile (ACN) in water and the supernatant diluted (1:1) with 100% ACN rather than resuspension was the optimal extraction protocol. Subsequently, the optimized method was able to well examine the change of metabolites in liver tissue resulting from mice treated with a toxic natural product toosendanin (TSN). Taken together, the optimized extraction and analytical protocol provides high reliability and reproducibility for polar and nonpolar metabolites in liver tissue and may be suitable for metabolomics analysis of liver injury‐induced by drugs or chemicals.

  • This paper is free to view for all users registered on spectroscopyNOW.com until the end of November 2018.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

Follow us on Twitter!

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2018 John Wiley & Sons, Inc. All Rights Reserved