Journal Highlight: Determination of trace amount of enantiomeric impurity in therapeutic nicotine derivative using CE with new imaging technology detection

Skip to Navigation

Ezine

  • Published: Jan 16, 2012
  • Channels: Detectors
thumbnail image: Journal Highlight: Determination of trace amount of enantiomeric impurity in therapeutic nicotine derivative using CE with new imaging technology detection

Determination of trace amount of enantiomeric impurity in therapeutic nicotine derivative using capillary electrophoresis with new imaging technology detection

Journal of Separation Science, 2012, 35, 66 - 72
Ali A. Dahab, Norman W. Smith

Abstract: One of the many attempts to stop the danger of tobacco smoking is the development of an anti-smoking vaccine using nicotine butyric acid (NBA) linked to a carrier protein to produce anti-nicotine antibodies. NBA is a chiral molecule and there is a need to obtain a high degree of enantiomeric purity. The aim of this work is to develop a novel method for the enantioseparation of NBA and the determination of trace amounts of enantiomeric impurity required by regulatory authorities. This was achieved successfully using high-performance capillary electrophoresis combined with label-free intrinsic imaging as new imaging technology. A 50 µm id fused-silica capillary was used with UV detection at 214 nm and label-free intrinsic imaging. The background electrolyte consisted of highly sulphated beta-cyclodextrin 10% m/V as a chiral selector in 75 mM phosphoric acid-triethylamine at pH 7.0. Baseline separation and detection of 0.1% and possibly less of the unwanted impurity (R-enantiomer) were achieved. Also, the detection limits were calculated for both enantiomers. The use of label-free intrinsic imaging has improved the sensitivity, enabling us to detect trace amounts of enantiomeric impurities. 

  • This paper is free to view for all users registered on separationsNOW.com until the end of March 2012.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.
Last ever cigarette 

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved