Pushing the Boundaries of Protein Identification,
Characterization and Quantitation Using a Novel
Orbitrap-Based Tribrid MS Architecture

Broadcast on October 1, 2013 
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Peptide identification rates and protein sequence coverage have historically advanced in line with improvements to the detection limits and spectral acquisition rate of the mass spectrometer. Spectral acquisition rates can be improved through extensive parallelization of the acquisition process using a novel Orbitrap mass spectrometer with a tribrid configuration of three mass analyzers.

This webinar demonstrates the advantages of using a novel Orbitrap mass spectrometer to dramatically improve spectral acquisition rates through massive parallelization of the acquisition process to enhance proteome coverage and general experimental throughput.

Taking the parallelization theme further, multiplexed quantitation using isobaric tags such as tandem mass tags (TMT) provides an avenue for mass spectrometry based proteome wide quantitation experiments. The novel arrangement of the three mass analyzers enables a new 'synchronous precursor scanning' MS3 method which provides a new level of sensitivity, precision and accuracy to multiplexed quantitation. Examples will be discussed ranging from fractionated colorectal cancer cell line lysates to low abundance phosphopeptide enriched samples.

Your Presenters:

Vlad Zabrouskov Manager, Product Management, Thermo Fisher Scientific Vlad did his graduate studies at Washington State University focusing on applying mass spectrometry to study plant lipid metabolism. He then joined the lab of Prof. McLafferty at Cornell University where he used top down FTICR-based mass spectrometry to characterize proteins involved in photosynthesis. After joining Thermo Fisher Scientific ten years ago, he worked on many instrument development projects including LTQ FT, LTQ Orbitrap, LTQ Velos, and Orbitrap Fusion mass spectrometers

Graeme McAlister Senior Engineer, Software, Thermo Fisher Scientific Graeme earned his PhD in Joshua Coon's lab, where he was involved in the development of ETD. Since then he has worked in the laboratory of Prof. Steven P. Gygi, where he has developed new methods to improve the accuracy, precision, sensitivity, and capacity of TMT-based multiplexed quantitation. A cornerstone of that work was the development of the MultiNotch MS3 method, which he will describe here today. Additionally, he will discuss how this quantitation method, in conjunction with the new Orbitrap Fusion, allows for multiplexed quantitation at sample depths that were previously unattainable.

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