Webinar
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High-coverage high-throughput characterization of breast cancer cell line proteomes using 10-plexed TMT on an Orbitrap Fusion

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  • Published: Mar 27, 2014
  • Author: Wilhelm Haas
  • Categories: Proteomics & Genomics / HPLC / Gas Chromatography / Base Peak / Atomic / Proteomics
thumbnail image: <font color=red>Webinar<br/>Now available on-demand</font><br/>High-coverage high-throughput characterization of breast cancer cell line proteomes using 10-plexed TMT on an Orbitrap Fusion


Thermo

High-coverage high-throughput characterization of breast cancer cell line proteomes using 10-plexed TMT on an Orbitrap Fusion



Broadcast on April 30, 2014

This webinar is now available on-demand.
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An ever growing amount of genomic and pharmacogenomic data on cancer tissue and cell lines reveals the enormous heterogeneity and complexity of the disease but it also holds the promise to inform new strategies for its targeted and patient-specific treatment. It is due to technical limitations that the “cancer”-proteome has yet remained largely untapped in these studies. However, we believe that this data has an immense potential to improve our understanding of the basic principles underlying cancer and to guide us to new approaches in treating the diseases as the proteome is a cell’s functional entity and virtually all targeted therapeutics in cancer treatment are targeting proteins.

In this webinar, we show that the use of TMT-10 reagents for quantitative proteomics on 32 breast cancer cell lines using a synchronous precursor selection supported MS3 method on the Thermo Scientific™ Orbitrap™ Fusion™ mass spectrometer allows us to overcome throughput limitations in quantitative proteomics by enabling the quantification of almost 8000 proteins in only 4.5 hours per cell line.

We believe that this technology puts proteomics in a position to be used side by side with genomics tools in studying complex diseases that require the analyses of a large number of samples.

Key learning objectives

  • Multiplexed quantitation using isobaric tags such as tandem mass tags (TMT)
  • The future of quantitative proteomics with respect to pathologies such as cancer
  • The use of TMT-10 reagents and the Thermo Scientific™ Orbitrap™ Fusion™ mass spectrometer to overcome throughput limitations

Who should attend?

  • Protein and peptide biochemists
  • Cancer cell biologists, biochemists, and biopharmaceutical scientists
  • Medical Geneticists
  • Mass spectrometrists
  • Molecular immunologists
  • Core lab directors
  • Anyone with interest in multiplexed quantitation


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In association with:
  
                                 Pittcon 2014                             Current Protocols

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