New running buffer offers faster protein separations

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  • Published: Apr 29, 2016
  • Author: Jon Evans
  • Source: Thermo Fisher Scientific
  • Suppliers: Thermo Fisher Scientific
  • Channels: Electrophoresis

A new running buffer from Thermo Fisher Scientific is designed for use at higher voltages than traditional buffers, providing gradient-like electrophoretic separation of protein samples in nearly one-third less time and over a broad molecular weight separation range.

When compared to traditional Tris-Glycine-SDS buffers, the new Fisher BioReagents FastRun Tris SDS PAGE Running Buffer provides comparable or better protein resolution and an increased molecular weight separation range. The new buffer system also reduces the number of Tris-Glycine polyacrylamide gel compositions required to resolve a protein, saving researchers time and money.

“For more than 40 years, the same buffer system has been used with Tris-Glycine polyacrylamide gels for high-resolution fractionation of protein mixtures,” said Stephen Roemer, director of product development at Thermo Fisher Scientific. “The traditional buffer system can be limiting for researchers because of the long run times and low voltage limits. The new Fisher BioReagents FastRun Tris SDS PAGE Running Buffer is the first major breakthrough in this space in decades because of its ability to be used at higher voltages.”

Tris-Glycine mini-gels (precast or home-made) prepared with conventional buffer systems are typically run at 125V. This is because the buffer system heats up at higher voltages, which in turn heats the gel, resulting in a breakdown of the protein bands and loss of resolution. The Fisher BioReagents FastRun buffer can be run at a higher voltage (200V recommended) because it does not generate excessive heat. The result is a significant improvement in run times, reducing 90-minute run times with traditional reagents to just 25 minutes with the new buffer system.

Fisher BioReagents FastRun buffer is compatible with standard Tris-Glycine polyacrylamide gels (precast or home-made) and with all commercially-available protein electrophoresis tanks.

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