Quantitative LC-MS/MS Glycomic Analysis Using Aminoxy Tandem Mass Tags

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Webinar

  • Date: Mar 3, 2015 - 16:00 - 17:00 (local time)
  • Presenter: Sergei Snovida and Yehia MechrefThermo
  • Categories: Proteomics & Genomics / Proteomics
thumbnail image: Quantitative LC-MS/MS Glycomic Analysis Using Aminoxy Tandem Mass Tags

Quantitative LC-MS/MS Glycomic Analysis
Using Aminoxy Tandem Mass Tags

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Broadcast on March 3, 2015


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Glycosylation plays important roles in biological processes, including modification of protein function, mediating biological turnover and facilitating cell-cell interaction. Glycans have been considered as potential biomarkers for many mammalian diseases. Mass spectrometry (MS)-based glycomics has become an integral part of glycobiology due to high sensitivity and robust characterization capabilities attained with modern MS instruments. Coupled with separation techniques, MS enables comprehensive glycomic quantification in complex biological samples. Although much advance in the area of quantitative glycomics has been made in recent years, several challenges still remain. In this work we investigate the use of recently introduced aminoxy Tandem Mass Tag (aminoxyTMT) reagents for quantitative LC-MS/MS glycomic analysis.

This webinar will provide an introduction to the concept of isobaric labeling of N-linked glycans with TMT reagents, with an emphasis on the labeling chemistry, sample preparation, and an overview of MS analysis workflows. The use of these reagents in biological systems will also be presented, including different cancer cell lines and different esophagus diseases.

Key Learning Objectives

  • Understand Mass Spectrometry based glycomics
  • Understand how Tandem Mass Tag (aminoxyTMT) reagents enable quantitative LC-MS/MS glycomic analysis
  • Receive an overview of MS analysis workflows with respect to glycomic analysis

Who should attend?

  • Mass spectrometrists
  • Researchers interested in MS based glycomics
  • Researchers interested in Tandem Mass Tag reagents for quantitative analysis

Your Presenters

Sergei Snovida

Sergei Snovida, Ph.D.

R&D Scientist with Mass
Spectrometry Reagents Group
Thermo Fisher Scientific

Sergei Snovida: Biography

Sergei obtained his Ph.D. in analytical chemistry from the Department of Chemistry at University of Manitoba (Winnipeg, Canada) in 2009. He then moved on to the Institute of Biological Chemistry of Academia Sinica (Taipei, Taiwan) where he worked as a post-doctoral fellow. In 2012, Sergei joined the mass spectrometry reagents group at Thermo Fisher Scientific as an R&D scientist.

Yehia Mechref

Yehia Mechref, Ph.D.

Professor of Bioanalytical Chemistry
Texas Tech University

Yehia Mechref: Biography

Yehia Mechref is a professor in the Department of Chemistry and Biochemistry at Texas Tech University, Lubbock. He received his BS in chemistry from the American University of Beirut (Beirut, Lebanon) and his PhD with an honorable mention from Oklahoma State University (Stillwater, Oklahoma).

Prof. Mechref research is focused on the development of sensitive biomolecular mass spectrometry methods enabling qualitative and quantitative assessments of proteins, glycoproteins and glycans in biological samples. Glycomic and glycoproteomic analyses remain to be analytically very challenging mainly due to the high complexity and microheterogeneity of glycosylation processes and machineries. The ultimate goal of the research activities of Dr. Mechref is the comprehensive characterization of glycoprotein glycosylation sites and microheterogeneity of such sites. Thus far, the research efforts of Prof. Mechref has culminated in the publication of over 162 papers and book chapters, including 16 review articles, 11 book chapters and over 126 peer-reviewed research papers. Currently, Dr. Mechref H-index is 40.

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In association with:
  
                                 Pittcon 2015                             Current Protocols

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