Journal Highlight: Detection and quantification of Escherichia coli and Pseudomonas aeruginosa in cow milk by near-infrared spectroscopy

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  • Published: Aug 15, 2015
  • Author: spectroscopyNOW
  • Channels: Infrared Spectroscopy
thumbnail image: Journal Highlight: Detection and quantification of <em>Escherichia coli</em> and <em>Pseudomonas aeruginosa</em> in cow milk by near-infrared spectroscopy
Near-IR spectroscopy was used to detect and discriminate between Escherichia coli and Pseudomonas aeruginosa in cow milk, to assess the degree of spoilage.

Detection and quantification of Escherichia coli and Pseudomonas aeruginosa in cow milk by near-infrared spectroscopy

International Journal of Dairy Technology, 2015, 68, 357-365
Fernando Cámara-Martos, Joao Almeida Lopes, Rafael Moreno-Rojas and Fernando Pérez-Rodríguez

Abstract: This work investigated the potential of NIR technology to be applied in the dairy industry for the detection of micro-organisms. To this end, two types of cow milk samples were studied, one in which only bacterial biomass was considered and the other in which bacteria were cultured and grown in milk for 24 h. The study was carried out using two micro-organisms Escherichia coli and Pseudomonas aeruginosa. Both types of samples with different counts of both micro-organisms were analysed by a NIR analyser in the range 10 000–4000 cm−1 based on transmittance measurements. Multivariate models indicated that a better discrimination between micro-organisms was attained in those milk samples in which micro-organisms have been grown.

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