Contact lens contaminating proteins identified

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  • Published: Jul 24, 2015
  • Author: Steve Down
  • Channels: Gas Chromatography / Ion Chromatography / Electrophoresis / Proteomics & Genomics / Sample Preparation / Detectors / Laboratory Informatics / HPLC / Raman / MRI Spectroscopy / NMR Knowledge Base / Base Peak / X-ray Spectrometry / Chemometrics & Informatics / Proteomics / Infrared Spectroscopy / UV/Vis Spectroscopy / Atomic

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As a contact lens wearer myself, I am painfully aware of the continuous need to keep lenses clean, in order to avoid physical discomfort and prevent inflammation. Proteins from the tear fluid are deposited on the lens surfaces within 10 minutes of wearing and continue to build up during the day. For lenses that are designed to be worn for more than one day, it is essential to soak them in cleaning solution overnight.

These proteins could provide information about the condition of the eye but, while there have been a number of studies on tear fluids from the eye, very few have been carried out on contact lens deposits. Now, Danish researchers have conducted a proteomics study to see which proteins are implicated, looking at the deposits on hydrogel contact lenses from three myopic patients, as they described in Acta Ophthalmologica.

Using conventional gel electrophoresis-mass spectrometric techniques, 72 protein spots were detected. Some of them were multiple spots from the same protein after post-translational modification, probably glycosylation, more than 12 of them being identified.

There were no surprises, but the experiments demonstrated that sufficient protein material can be collected, even after one hour, to be studied. So, individual patients can be examined. However, for analysis on multiple gels, the extracted proteins were pooled.

The study illustrates a novel way of studying ocular conditions. In this case, the patients were all myopic the scheme could be applied equally to other conditions such as dry eye syndrome and Sjogren’s syndrome.

Comments

1. At 07:08 on Jul 30, 2015, Steven Rios wrote:

Using the gel electrophoresis-mass spectrometric techniques, and protein material can be collected, and even to be studied after one hour, well, your post is very helpful for me. 

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