Journal Highlight: MALDI-MS investigation of pollen and their classification by multivariate statistics

Skip to Navigation

Ezine

  • Published: May 14, 2012
  • Author: spectroscopyNOW
  • Channels: Base Peak
thumbnail image: Journal Highlight: MALDI-MS investigation of pollen and their classification by multivariate statistics

Matrix-assisted laser desorption/ionization mass spectrometric investigation of pollen and their classification by multivariate statistics

Rapid Communications in Mass Spectrometry, 2012, 26, 1032-1038
Benjamin Krause, Stephan Seifert, Ulrich Panne, Janina Kneipp, Steffen M. Weidner

Matrix-assisted laser desorption/ionization mass spectrometry was applied to the analysis of extracts and milled pollen samples and the data were explored for characteristic peak patterns which could be subjected to a multivariate statistical analysis. Abstract: A fast and reliable online identification of pollen is not yet available. The identification of pollen is based mainly on the evaluation of morphological data obtained by microscopic methods. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) was applied to the analysis of extracts and milled pollen samples. The obtained MALDI data were explored for characteristic peak patterns which could be subjected to a multivariate statistical analysis. Two sample preparation methods are presented, which require only minimal or no chemical extraction of the pollen. MALDI pollen spectra could be recorded showing various peak patterns. A multivariate statistics approach allowed the classification of pollen into clusters indicating similarities and differences between various species. These results demonstrate the potential and the reliability of MALDI-TOF MS for the identification and, in combination with multivariate statistics, also for the classification of pollen.

  • This paper is free to view for all users registered on separationsNOW.com until the end of June 2012.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved