Pharmaceuticals and Personal Care Products in Water, Soil, Sediment, and Biosolids by HPLC/MS/MS: EPA Method 1694

Pharmaceuticals and Personal Care Products in Water, Soil, Sediment, and Biosolids by HPLC/MS/MS - EPA Method 1694

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Presented by E. Michael Thurman Director, Center for Environmental Mass Spectrometry Dept. of Civil, Environmental, and Architectural Engineering University of Colorado Boulder, CO USA

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This complimentary seminar focuses on the utilization of HPLC/MS/MS within EPA Method 1694 guidelines. Since the discovery of persistent pharmaceuticals in wastewater and their widespread occurrence, numerous papers have appeared on the analysis of these compounds. EPA has established a method, EPA 1694, to evaluate these compounds in many environmental matrices. We have tested this EPA method and report on our results. Thus, an analytical methodology for screening and confirming the presence of 74 pharmaceuticals in water samples was developed using a triple quadrupole mass spectrometer. The method was developed following the guidelines in EPA Method 1694. Four distinct chromatographic gradients and LC conditions were used according to the polarity and extraction of the different pharmaceuticals. Positive and negative ion electrospray were used with two MRM transitions (a quantifier and a qualifier ion for each compound), which adds extra confirmation in this methodology compared with the EPA method. Linearity of response of 3 orders of magnitude was demonstrated (r² > 0.99) for all the pharmaceuticals studied. The analytical performance of the method was evaluated for one wastewater sample collected in Boulder Creek, Colorado; positive identifications for carbamazepine and diphenhydramine were found for this sample using the methodology developed in this work. This study is a valuable indicator of the potential of the QqQ for routine quantitative multi-residue analysis of pharmaceuticals in water and wastewater samples.

Attendees will benefit from this live session with Dr. Thurman as he presents the results of using HPLC/MS/MS to meet EPA Method 1694 guidelines.