Journal Highlight: 31P NMR of the pyruvate kinase reaction: An undergraduate experiment in enzyme kinetics

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  • Published: Nov 27, 2017
  • Author: spectroscopyNOW
  • Channels: NMR Knowledge Base
thumbnail image: Journal Highlight: <sup>31</sup>P NMR of the pyruvate kinase reaction: An undergraduate experiment in enzyme kinetics

A simple 31P NMR tube experiment has been developed that allows students to examine the enzyme kinetics and equilibrium constant of the reaction catalyzed by pyruvate kinase.

31P NMR of the pyruvate kinase reaction: An undergraduate experiment in enzyme kinetics

Biochemistry and Molecular Biology Education, 2017, 45, 509-514
R. Marshall Werner and Austin Johnson

Abstract: Understanding how to perform an enzyme assay is a critical learning skill in the undergraduate biochemistry curriculum. Students in biochemistry typically have been exposed to the use of NMR spectroscopy as a tool to determine chemical structure, but rarely are they exposed to the utility of NMR to evaluate enzyme kinetics. Furthermore, coverage of NMR experiments utilizing "alternative nuclei", such as 15N, 19F, and 31P may be neglected. Herein we report a simple 31P NMR tube experiment that allows students to examine the enzyme kinetics and equilibrium constant of the reaction catalyzed by pyruvate kinase.

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