Journal Highlight: Tissue proteomics of the low-molecular weight proteome using an integrated cLC-ESI-QTOFMS approach

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  • Published: Jun 24, 2013
  • Author: spectroscopyNOW
  • Channels: Proteomics
thumbnail image: Journal Highlight: Tissue proteomics of the low-molecular weight proteome using an integrated cLC-ESI-QTOFMS approach
A tissue proteomics approach involving tissue homogenization followed by depletion of large proteins and then capillary LC-MS analysis has been evaluated for examining the low molecular weight/low abundance tissue proteome.

Tissue proteomics of the low-molecular weight proteome using an integrated cLC-ESI-QTOFMS approach

Proteomics, 2013, 13, 1400-1411
MeiHwa Tanielle Bench Alvarez, Dipti Jigar Shah, Craig D. Thulin, Steven W. Graves

Abstract: Analysis of the protein/peptide composition of tissue has provided meaningful insights into tissue biology and even disease mechanisms. However, little has been published regarding top down methods to investigate lower molecular weight (MW) (500–5000 Da) species in tissue. Here, we evaluate a tissue proteomics approach involving tissue homogenization followed by depletion of large proteins and then cLC-MS (where c stands for capillary) analysis to interrogate the low MW/low abundance tissue proteome. In the development of this method, sheep heart, lung, liver, kidney, and spleen were surveyed to test our ability to observe tissue differences. After categorical tissue differences were demonstrated, a detailed study of this method's reproducibility was undertaken to determine whether or not it is suitable for analyzing more subtle differences in the abundance of small proteins and peptides. Our results suggest that this method should be useful in exploring the low MW proteome of tissues.

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