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Novel top-down protein platform: Serial SEC with FTICR mass spectrometry

Date: Mar 6, 2019

Author: Steve Down

Mixtures of intact proteins with closely related molecular masses that were extracted from heart tissue have been resolved and identified by high-resolution FT/ICR mass spectrometry following serial size exclusion chromatography.

Read More thumbnail image: Novel top-down protein platform Serial SEC with FTICR mass spectrometry

Journal Highlight: Integrating clinical, molecular, proteomic and histopathological data within the tissue context: Tissunomics

Date: Feb 25, 2019

Author: spectroscopyNOW

The term tissunomics has been proposed to define a system that integrates both morphological, molecular and protein information for the correct evaluation of tumors in a clinical and pathological context, where intratumoral heterogeneity can be assessed.

Read More thumbnail image: Journal Highlight Integrating clinical molecular proteomic and histopathological data within the tissue context Tissunomics

Glycopeptide enrichment for mass spectrometry: High-throughput for prostate cancer screening

Date: Feb 19, 2019

Author: Steve Down

A rapid system using titanium dioxide beads for purifying glycopeptides derived from proteins in human serum has been applied to the screening of suspected prostate cancer samples and is suitable for high-throughput analysis.

Read More thumbnail image: Glycopeptide enrichment for mass spectrometry High-throughput for prostate cancer screening

Fungi on the International Space Station: Modified proteome

Date: Feb 6, 2019

Author: Steve Down

The proteomes of two strains of the microorganism Aspergillus fumigatus, which were isolated from the International Space Station, have been analysed for the first time, revealing potential adaptations to the unique environment that might impact on the health of astronauts.

Read More thumbnail image: Fungi on the International Space Station Modified proteome

Journal Highlight: Application of extracellular vesicles proteomics to cardiovascular disease: Guidelines, data analysis, and future perspectives

Date: Jan 29, 2019

Author: spectroscopyNOW

Some guidelines for the isolation, characterization, and proteomic analysis of plasma‐derived extracellular vesicles have been reviewed in the context of cardiovascular disease studies.

Read More thumbnail image: Journal Highlight Application of extracellular vesicles proteomics to cardiovascular disease Guidelines data analysis and future perspectives

Kawasaki disease: Protein biomarker candidates

Date: Jan 17, 2019

Author: Steve Down

The rare but serious condition known as Kawasaki disease, which mainly affects children under the age of five, produces a modified urinary protein profile that could lead to the development of a diagnostic panel of proteins.

Read More thumbnail image: Kawasaki disease Protein biomarker candidates

Preeclampsia proteins: Placental biomarkers

Date: Jan 3, 2019

Author: Steve Down

A proteomics analysis of the placental proteins of pregnant women with preeclampsia has revealed 37 proteins with altered abundances which could be used to develop diagnostic tests or treatments for this disease.

Read More thumbnail image: Preeclampsia proteins Placental biomarkers

Mining the human proteome: Deeper coverage to find missing proteins

Date: Dec 17, 2018

Author: Steve Down

The depth of coverage of the human proteome can be markedly extended by introducing a preliminary alkaline 2D-LC step before analysis by LC/MS with selected reaction monitoring using isotope-labelled standards or shotgun LC/MS/MS analysis.

Read More thumbnail image: Mining the human proteome Deeper coverage to find missing proteins

Journal Highlight: A proteomics and other omics approach in the context of farmed fish welfare and biomarker discovery

Date: Dec 17, 2018

Author: spectroscopyNOW

Current methods and assays used to measure animal and farmed fish welfare have been discussed and the introduction of a new type of welfare biomarkers using cutting‐edge technologies like proteomics and other omics technologies have been reviewed.

Read More thumbnail image: Journal Highlight A proteomics and other omics approach in the context of farmed fish welfare and biomarker discovery

Paediatric kidney transplant rejection: Peptide biomarkers

Date: Dec 1, 2018

Author: Steve Down

Kidney transplant rejection in children, specifically chronic active antibody-mediated rejection (cAMBR), can be predicted by a panel of urinary peptides, many derived from collagen species, a development that could indicate those patients who would benefit from increased immunosuppressive treatment.

Read More thumbnail image: Paediatric kidney transplant rejection Peptide biomarkers
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