Proteomics & Genomics / Ezine
Microgravity modifies proteome: Dry immersion model for weightlessness reveals irreversible protein changes
Date: Apr 1, 2011
Author: Steve Down
Dry immersion, which is used to simulate weightlessness over periods of several days, induces irreversible changes in the human serum proteome that cannot be alleviated by mechanical or myostimulation, say Russian scientists. The hormonal, immune and lipid systems are all affected.
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Journal Highlight: Quantitative proteomics for investigating psychiatric disorders
Date: Mar 22, 2011
Author:
Quantitative proteomic approaches were discussed for elucidating molecular mechanisms of psychiatric disorders such as anxiety, schizophrenia, bipolar disorder and depression, by studying specimens from animal models and patients using gel-based, label-free and stable isotope-labeling methodologies.
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Down's syndrome development: the case for oxidative stress
Date: Mar 15, 2011
Author: Steve Down
A set of oxidised proteins has been identified in the amniotic fluid of women carrying Down's syndrome babies, confirming the early onset of oxidative stress in the disease which might contribute to the development of different disease phenotypes.
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Tackling trypsin weakness: large peptides harvested by SEC for secondary digestion
Date: Mar 1, 2011
Author: Steve Down
Coverage of the proteome can be extended significantly following tryptic digestion by isolating the fraction of large peptides by size exclusion chromatography for further digestion with other enzymes.
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Journal Highlight: Reconstructing the pipeline by introducing multiplexed MRM-MS for cancer biomarker verification: An NCI-CPTC initiative perspective
Date: Feb 21, 2011
Author:
The technology of proteomics and its application to transfusion medicine is reviewed, with specific reference to the analysis of blood products, both fractionated and fresh.
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Deer antler proteome: the value of parallel extraction methods
Date: Feb 15, 2011
Author: Steve Down
The proteome of deer antlers has been extended more than three-fold by scientists in China, who used a series of different extraction solutions to widen the range of proteins obtained, followed by liquid chromatography-tandem mass spectrometry with serially coupled HPLC columns to improve resolution.
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Aging proteins: Redox proteins implicated in longevity
Date: Feb 1, 2011
Author: Steve Down
A proteomics analysis of the plasma of ten Japanese women over 105 years old has implicated proteins involved in oxidative stress regulation in the pursuit of longevity.
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Journal Highlight: Reconstructing the pipeline by introducing multiplexed MRM-MS for cancer biomarker verification: An NCI-CPTC initiative perspective
Date: Jan 24, 2011
Author:
The use of multiple reaction monitoring in mass spectrometry, with or without affinity enrichment, is reviewed as a sensitive, reproducible and quantitative platform for cancer biomarker verification.
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Brain death predictors: proteomics reveals molecular safeguards against death
Date: Jan 15, 2011
Author: Steve Down
The proteome of an area of the brain that has been associated with brain death is distinctively different from that of another part of the brain, say Chinese scientists, with raised levels of antioxidant proteins in particular. These findings will help to establish the origins of brain death.
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Discolouration of cooked potatoes: proteomics points to a solution
Date: Jan 1, 2011
Author: Steve Down
The wound response proteins associated with the black discolouration of potatoes following cooking have been identified by quantitative proteomics and cluster analysis, which should help to produce varieties with a reduced darkening tendency.
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