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Trypsin autolysis reduced: In-gel digestion improved

Date: Aug 15, 2014

Author: Steve Down

The amount of trypsin used in typical in-gel digestion procedures can be reduced ten-fold at least, without any loss of performance. In fact, some aspects are improved, such as a reduction in the number of trypsin autolysis products and a notable increase in the number of proteins identified in complex samples.

Read More thumbnail image: Trypsin autolysis reduced In-gel digestion improved

Swine flu biomarkers: Plasma proteins mark H1N1 infection

Date: Aug 4, 2014

Author: Steve Down

Korean scientists have found a number of proteins that have altered abundances in human plasma from people infected with the H1N1 influenza virus, with the potential to become biomarkers of infection.

Read More thumbnail image: Swine flu biomarkers Plasma proteins mark H1N1 infection

Journal Highlight: Blessing or curse? Proteomics in granzyme research

Date: Jul 21, 2014

Author: spectroscopyNOW

This review aims to summarize and analyze the different proteomic approaches used to study granzymes and discusses both the convincing and controversial outcomes.

Read More thumbnail image: Journal Highlight Blessing or curse Proteomics in granzyme research

Insulin undone: Endogenous and synthetic forms measured in plasma

Date: Jul 20, 2014

Author: Steve Down

Natural insulin and its synthetic variants present in the human body can be distinguished by mass spectrometry following global immunoaffinity extraction, a technique that will be useful in clinical, forensic and sports doping settings.

Read More thumbnail image: Insulin undone Endogenous and synthetic forms measured in plasma

Canine proteome: Human disease model

Date: Jul 3, 2014

Author: Steve Down

The canine urinary proteome has been characterised in the US and compared to that of human urine, to see if it can act as a model for the study of human diseases.

Read More thumbnail image: Canine proteome Human disease model

Journal Highlight: Recent advances in proteomics: towards the human proteome

Date: Jun 23, 2014

Author: spectroscopyNOW

This review summarizes the key discoveries that laid down the foundations for proteomics as we now know it, and describes key recent technological advances that will undoubtedly contribute to achieving the initial goal of the Human Proteome Organization.

Read More thumbnail image: Journal Highlight Recent advances in proteomics towards the human proteome

Colourful catfish: Protein clues to red tinge

Date: Jun 15, 2014

Author: Steve Down

Catfish fillets gain their reddish off-colour after being caught due to an excess of haemoglobin possibly brought on by stress, say researchers in the US.

Read More thumbnail image: Colourful catfish Protein clues to red tinge

Autistic proteins: Cholesterol metabolism and oxidative stress implicated

Date: Jun 2, 2014

Author: Steve Down

A transatlantic group of scientists has identified several proteins involved in cholesterol metabolism that have increased abundances in children with autism spectrum disorder, supporting speculation that oxidative stress might be a factor behind this condition.

Read More thumbnail image: Autistic proteins Cholesterol metabolism and oxidative stress implicated

Journal Highlight: Pathophysiology of tobacco smoke exposure: Recent insights from comparative and redox proteomics

Date: May 26, 2014

Author: spectroscopyNOW

Different quantitative and redox proteomic approaches that have been used to evaluate changes in protein expression and specific oxidative protein modifications induced by exposure to cigarette smoke are overviewed.

Read More thumbnail image: Journal Highlight Pathophysiology of tobacco smoke exposure Recent insights from comparative and redox proteomics

Blood sampling for proteomics: Anticoagulant comparison

Date: May 17, 2014

Author: Steve Down

The proteolytic profiles of human plasma treated with different anticoagulants and of untreated human serum have been compared in order to define the effects encountered during clinical proteomics and biomarker studies.

Read More thumbnail image: Blood sampling for proteomics Anticoagulant comparison
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