Proteomics & Genomics / Ezine
Growth hormone detection
Date: Sep 1, 2009
Author: Steve Down
The administration of recombinant human growth hormone to humans can be accomplished using a panel of six protein ions identified by MALDI MS or six peptide ions identified by LC/MS, say researchers in the UK. Their study combined proteomics with artificial neural networks in the first study of its type in biomarker discovery.
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Proteomic characterisation of subclover seed storage proteins during germination
Date: Aug 24, 2009
Author:
The seed storage proteome of the non-model species (Trifolium subterraneum), was characterised in mature dry seeds and during imbibition using two-dimensional electrophoresis coupled with tandem mass spectrometry.
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Oxidised Alzheimer's proteins identified
Date: Aug 15, 2009
Author: Steve Down
Specific brain proteins modified by lipid peroxidation products in a region-specific manner have been identified in Alzheimer's disease patients. The two regions examined are highly susceptible, linking oxidative damage to neurodegeneration.
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Bolts from nuts
Date: Aug 1, 2009
Author: Steve Down
The allergen contents of different varieties of peanut have been compared by proteomics techniques, revealing the existence of hitherto unreported fragments of major allergens. Indonesian nuts were found to contain lower amounts of major allergens, which has implications for food production.
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Evolution of nacre: Biochemistry and proteomics of the shell organic matrix of the cephalopod Nautilus macromphalus
Date: Jul 27, 2009
Author:
The nacre matrix of the cephalopod Nautilus macromphalus has been biochemically characterized, in part by a proteomic approach applied to the acetic acid-soluble and -insoluble shell matrices and the spots obtained after 2D gel electrophoresis. Most of the obtained partial sequences are entirely new.
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Making horse sense
Date: Jul 15, 2009
Author: Steve Down
The effects of equine drugs of abuse on the levels of proteins in horse plasma have been studied by scientists in the UK, leading to the discovery of candidate protein biomarkers for doping. These candidates were detected and measured using targeted analysis of their tryptic peptides.
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Obesity and LDL proteins
Date: Jul 1, 2009
Author: Steve Down
Obesity is associated with changes in the protein composition of low-density lipoprotein (LDL), as well as high levels of LDL cholesterol, according to scientists in Sweden. The transport protein transthyretin was found to be enriched in LDL from obese subjects and may be linked to an increased risk of cardiovascular disease.
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Proteomics in globe artichoke: Protein extraction and sample complexity reduction by PEG fractionation
Date: Jun 22, 2009
Author:
The first leaf proteome analysis for globe artichoke is reported, removing the highly abundant leaf protein RuBisCO to improve spot resolution of the less abundant proteins. In all, 841 distinct protein spots were detected.
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Prospective biomarkers are too common
Date: Jun 15, 2009
Author: Steve Down
A review of proteomics studies in the literature covering five species has revealed a common set of differentially expressed proteins, representing a set of regularly detected protein families. Many are linked to stress response, and their use as biomarkers of any other condition should be considered with caution.
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Stressed out
Date: Jun 1, 2009
Author: Steve Down
The metabolic pathways of rats subjected to acute and chronic stress varied in the same way but the changes were reversible once the stress was withdrawn. Chronic stress also induced behavioural and physiological signs of depression which were supported by changes in the levels of metabolites associated with depression.
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