Proteomics & Genomics / Ezine
Proteomics of cold-adapted microorganism: three distinct physiological states exist over growth temperature range
Date: May 2, 2011
Author: Steve Down
The response of the proteome of a cold-adapted microorganism to temperatures across its complete growth range has revealed three physiological states corresponding to cold stress, cold adaptation and heat stress.
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Journal Highlight: Toward a standardized urine proteome analysis methodology
Date: Apr 25, 2011
Author:
A systematic study has been undertaken to define standardized and integrated analytical protocols for a biomarker development pipeline, employing two LC-MS analytical platforms, namely accurate mass and time tags and selected reaction monitoring, for the discovery and verification phase, respectively.
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Immunodepletion explored: biomarker discovery assisted by increased depletion
Date: Apr 13, 2011
Author: Steve Down
A systematic study of the immunodepletion of clinical serum samples using commercial columns in the search for biomarkers revealed that subsequent bottom-up experiments with a high-resolution mass spectrometer showed excellent reproducibility and a wider range of identified proteins than 2D differential gel electrophoresis.
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Microgravity modifies proteome: Dry immersion model for weightlessness reveals irreversible protein changes
Date: Apr 1, 2011
Author: Steve Down
Dry immersion, which is used to simulate weightlessness over periods of several days, induces irreversible changes in the human serum proteome that cannot be alleviated by mechanical or myostimulation, say Russian scientists. The hormonal, immune and lipid systems are all affected.
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Journal Highlight: Quantitative proteomics for investigating psychiatric disorders
Date: Mar 22, 2011
Author:
Quantitative proteomic approaches were discussed for elucidating molecular mechanisms of psychiatric disorders such as anxiety, schizophrenia, bipolar disorder and depression, by studying specimens from animal models and patients using gel-based, label-free and stable isotope-labeling methodologies.
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Down's syndrome development: the case for oxidative stress
Date: Mar 15, 2011
Author: Steve Down
A set of oxidised proteins has been identified in the amniotic fluid of women carrying Down's syndrome babies, confirming the early onset of oxidative stress in the disease which might contribute to the development of different disease phenotypes.
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Tackling trypsin weakness: large peptides harvested by SEC for secondary digestion
Date: Mar 1, 2011
Author: Steve Down
Coverage of the proteome can be extended significantly following tryptic digestion by isolating the fraction of large peptides by size exclusion chromatography for further digestion with other enzymes.
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Journal Highlight: Reconstructing the pipeline by introducing multiplexed MRM-MS for cancer biomarker verification: An NCI-CPTC initiative perspective
Date: Feb 21, 2011
Author:
The technology of proteomics and its application to transfusion medicine is reviewed, with specific reference to the analysis of blood products, both fractionated and fresh.
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Deer antler proteome: the value of parallel extraction methods
Date: Feb 15, 2011
Author: Steve Down
The proteome of deer antlers has been extended more than three-fold by scientists in China, who used a series of different extraction solutions to widen the range of proteins obtained, followed by liquid chromatography-tandem mass spectrometry with serially coupled HPLC columns to improve resolution.
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Aging proteins: Redox proteins implicated in longevity
Date: Feb 1, 2011
Author: Steve Down
A proteomics analysis of the plasma of ten Japanese women over 105 years old has implicated proteins involved in oxidative stress regulation in the pursuit of longevity.
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