Journal Highlight: In vivo probing of the temperature responses of intracellular biomolecules in yeast cells by label-free Raman microspectroscopy

Image: Jef Boeke and Sarah Richardson, Johns Hopkins University

In vivo probing of the temperature responses of intracellular biomolecules in yeast cells by label-free Raman microspectroscopy

ChemBioChem, 2013, 14, 1001-1005
Yu-Fang Chiu, Chuan-Keng Huang, Shinsuke Shigeto

Abstract: Environmental temperature is an essential physical quantity that substantially influences cell physiology by changing the equilibria and kinetics of biochemical reactions occurring in cells. Although it has been extensively used as a readily controllable parameter in genetic and biochemical research, much remains to be explored about the temperature responses of intracellular biomolecules in vivo and at the molecular level. Here we report in vivo probing, achieved with label-free Raman microspectroscopy, of the temperature responses of major intracellular components such as lipids and proteins in living fission yeast cells. The characteristic Raman band at 1602 cm⁻¹, which has been attributed mainly to ergosterol, showed a significant decrease (≈47 %) in intensity at elevated temperatures above 35 °C. In contrast to this high temperature sensitivity of the ergosterol Raman band, the phospholipid and protein Raman bands did not vary much with increasing culture temperature in the 26–38 °C range. This finding agrees with a previous biochemical study that showed that the initial stages of ergosterol biosynthesis in yeast are hindered by temperature elevation. Moreover, our result demonstrates that Raman microspectroscopy holds promise for elucidation of temperature-dependent cellular activities in living cells, with a high molecular specificity that the commonly used fluorescence microscopy cannot offer.

• This paper is free to view for all users registered on spectroscopyNOW.com until the end of July 2013.
  After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

• Click here to access the abstract of this paper >>>
  From the abstract page you can progress to read the full paper.

• Click here for more information about ChemBioChem >>>