Journal Highlight: Distinguishing individual DNA bases in a network by non-resonant tip-enhanced Raman scattering

Skip to Navigation

Ezine

  • Published: Jun 15, 2017
  • Author: spectroscopyNOW
  • Channels: Raman
thumbnail image: Journal Highlight: Distinguishing individual DNA bases in a network by non-resonant tip-enhanced Raman scattering

Scanning tunneling microscope-controlled non-resonant TERS has been used to distinguish unambiguously between two individual complementary DNA bases (adenine and thymine) with a spatial resolution down to 0.9 nm.

Distinguishing individual DNA bases in a network by non-resonant tip-enhanced Raman scattering

Angewandte Chemie International Edition, 2017, 56, 5561-5564
Rui Zhang, Xianbiao Zhang, Huifang Wang, Yao Zhang, Song Jiang, Chunrui Hu, Yang Zhang, Yi Luo and Zhenchao Dong

Abstract: The importance of identifying DNA bases at the single-molecule level is well recognized for many biological applications. Although such identification can be achieved by electrical measurements using special setups, it is still not possible to identify single bases in real space by optical means owing to the diffraction limit. Herein, we demonstrate the outstanding ability of scanning tunneling microscope (STM)-controlled non-resonant tip-enhanced Raman scattering (TERS) to unambiguously distinguish two individual complementary DNA bases (adenine and thymine) with a spatial resolution down to 0.9 nm. The distinct Raman fingerprints identified for the two molecules allow to differentiate in real space individual DNA bases in coupled base pairs. The demonstrated ability of non-resonant Raman scattering with super-high spatial resolution will significantly extend the applicability of TERS, opening up new routes for single-molecule DNA sequencing.

  • This paper is free to view for all users registered on spectroscopyNOW.com until the end of August 2017.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

Follow us on Twitter!

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved