Journal Highlight: Rapid detection of ricin in milk using immunomagnetic separation combined with surface-enhanced Raman spectroscopy

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  • Published: Jun 6, 2011
  • Channels: Raman
thumbnail image: Journal Highlight: Rapid detection of ricin in milk using immunomagnetic separation combined with surface-enhanced Raman spectroscopy

Rapid detection of ricin in milk using immunomagnetic separation combined with surface-enhanced Raman spectroscopy

Journal of Food Science, 2011, 76, N49-N53
Lili He, Bronwyn Deen, Tom Rodda, Ian Ronningen, Tim Blasius, Christy Haynes, Francisco Diez-Gonzalez, Theodore P. Labuza

Abstract: Ricin is a potential bioterrisiom agent. There is a critical need for a method that can rapidly and simply detect ricin and other bioterrisiom agents in complex food matrices such as milk. In this study, we demonstrated a rapid method that combined immunomagnetic separation (IMS) and surface-enhanced Raman spectroscopy (SERS) to detect ricin in whole milk. IMS was used to specifically capture the ricin out of the milk. Then, SERS was applied to analyze the IMS eluate mixed with silver dendrite nanosubstrates. This approach facilitated detection and quantification down to 4 µg/mL ricin in milk within 20 min, based on the results of principal component analysis and partial least squares analysis. The feasibility of using a portable Raman instrument shows great promise for on-site detection in a processing facility.

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A rapid method that combined immunomagnetic separation and surface-enhanced Raman spectroscopy has been demonstrated for the detection of ricin in whole milk, the feasibility of a portable Raman instrument showing great promise for on-site detection in a processing facility

 

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