Journal Highlight: Brilliant violet fluorophores: A new class of ultrabright fluorescent compounds for immunofluorescence experiments

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  • Published: Jun 11, 2012
  • Channels: UV/Vis Spectroscopy
thumbnail image: Journal Highlight: Brilliant violet fluorophores: A new class of ultrabright fluorescent compounds for immunofluorescence experiments

Brilliant violet fluorophores: A new class of ultrabright fluorescent compounds for immunofluorescence experiments

Cytometry Part A, 2012, 81A, 456-466
Pratip K. Chattopadhyay, Brent Gaylord, Adrian Palmer, Nan Jiang, Mary A. Raven, Geoff Lewis, Morgan A. Reuter, A.K.M. Nur-ur Rahman, David A. Price, Michael R. Betts, Mario Roederer

The first uses of a new class of “Brilliant Violet” fluorescent probes based on conductive organic polymers are reported in a diverse range of cytometric and imaging applications. Abstract: The Nobel Prize in Chemistry was awarded in 2000 for the discovery of conductive organic polymers, which have subsequently been adapted for applications in ultrasensitive biological detection. Here, we report the first use of this new class of fluorescent probes in a diverse range of cytometric and imaging applications. We demonstrate that these “Brilliant Violet” reporters are dramatically brighter than other UV-violet excitable dyes, and are of similar utility to phycoerythrin (PE) and allophycocyanin (APC). They are thus ideally suited for cytometric assays requiring high sensitivity, such as MHC-multimer staining or detection of intracellular antigens. Furthermore, these reporters are sensitive and spectrally distinct options for fluorescence imaging, two-photon microscopy and imaging cytometry. These ultra-bright materials provide the first new high-sensitivity fluorescence probes in over 25 years and will have a dramatic impact on the design and implementation of multicolor panels for high-sensitivity immunofluorescence assays.

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