Journal Highlight: Spectroscopic methods and their applicability for high-throughput characterization of mammalian cell cultures in automated cell culture systems

Skip to Navigation

Ezine

  • Published: Aug 8, 2016
  • Author: spectroscopyNOW
  • Channels: UV/Vis Spectroscopy
thumbnail image: Journal Highlight: Spectroscopic methods and their applicability for high-throughput characterization of mammalian cell cultures in automated cell culture systems
UV/VIS, fluorescence, Raman, near-infrared, and mid-infrared spectroscopy are presented and evaluated for their applicability in the new field of mammalian cell culture processes in automated cell culture systems.

Spectroscopic methods and their applicability for high-throughput characterization of mammalian cell cultures in automated cell culture systems

Engineering in Life Sciences, 2016, 16, 405-416
Carsten Musmann, Klaus Joeris, Sven Markert, Dörte Solle and Thomas Scheper

Abstract: The number and use of automated cell culture systems for mammalian cell culture are steadily increasing. Automated cell culture systems require miniaturized analytics with a high throughput to obtain as much information as possible from single experiments. Standard analytics commonly used for conventional bioreactor samples cannot handle the high throughput and the low sample volumes. Spectroscopic methods provide a means of meeting this analytical requirement and afford fast and direct access to process information. In the first part of this review, UV/VIS, fluorescence, Raman, near-infrared, and mid-infrared spectroscopy are presented. In the second part of the review, these spectroscopic methods are evaluated in terms of their applicability in the new field of mammalian cell culture processes in automated cell culture systems. Unlike standard bioreactors, these automated systems have special requirements that apply to the use of spectroscopic methods. Therefore, they are compared with regard to cell culture automation, throughput, and required sample volume.

  • This paper is free to view for all users registered on spectroscopyNOW.com until the end of September 2016.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

Follow us on Twitter!

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved