Journal Highlight: Haploid differentiation in maize kernels based on fluorescence imaging

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  • Published: Sep 6, 2016
  • Author: spectroscopyNOW
  • Channels: UV/Vis Spectroscopy
thumbnail image: Journal Highlight: Haploid differentiation in maize kernels based on fluorescence imaging
<p>A new fluorescence-based method for inbred haploid differentiation in maize kernels was developed by utilizing the <em>R1-nj</em> colour marker in combination with fluorescence microspectroscopy and imaging.</p>

Haploid differentiation in maize kernels based on fluorescence imaging

Plant Breeding, 2016, 135, 439-445
Brett W. Boote, Daniel J. Freppon, Gerald N. De La Fuente, Thomas Lübberstedt, Basil J. Nikolau and Emily A. Smith

Abstract: A new fluorescence-based method for inbred haploid differentiation in maize kernels was developed by utilizing the R1-nj colour marker in combination with fluorescence microspectroscopy and imaging. Seven inbred lines with varying R1-nj expression were used in this study. The fluorescence response of the diploid kernels at the embryonic dye spot was shown to simultaneously exhibit lower intensity and occur at a higher wavelength than the fluorescence of the dye-lacking haploid embryos. Intensity and area thresholds were applied to fluorescence images to sort the haploids from mixed sample populations, and sorting efficiencies of greater than 80% were achieved in all seven inbred lines (with values greater than 90% for five lines). The potential for high-throughput sorting when fluorescence imaging is combined with existing technologies for seed handling as well as high sorting efficiency may make fluorescence a viable and promising alternative to current sorting methods for some inbred lines.

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