Journal Highlight: Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell

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  • Published: Sep 6, 2011
  • Channels: UV/Vis Spectroscopy
thumbnail image: Journal Highlight: Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell

Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell

Microscopy Research and Technique 2011, 74, 788-793
Sergi Padilla-Parra, Nicolas Auduge, Maïte Coppey-Moisan, Marc Tramier

Abstract: Dual-color fluorescence correlation spectroscopy is an interesting method to quantify protein interaction in living cells. But, when performing these experiments, one must compensate for a known spectral bleed through artifact that corrupts cross-correlation data. In this article, problems with crosstalk were overcome with an approach based on fluorescence lifetime correlation spectroscopy (FLCS). We show that FLCS applied to dual-color EGFP and mCherry cross-correlation allows the determination of protein-protein interactions in living cells without the need of spectral bleed through calibration. The methodology was validated by using EGFP-mCherry tandem in comparison with coexpressed EGFP and mCherry in live cell. The dual-color FLCS experimental procedure where the different laser intensities do not have to be controlled during experiment is really very helpful to study quantitatively protein interactions in live sample.

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Problems with crosstalk in dual-color fluorescence correlation spectroscopy studies of protein interactions in living cells have been overcome with an approach based on fluorescence lifetime correlation spectroscopy 

 

 

 

 

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