UV/Vis Spectroscopy / Ezine
Cast-iron protection racket
Date: Jul 1, 2009
Author: David Bradley
Little is known about how the haem group that carries oxygen in blood and is the active centre of several enzymes is transported from where it is made in the cell to its host protein assembly. Now, UV-Vis spectroscopy has helped identify an enzyme that also functions as a haem transporter.
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Fluorescence microscopy and flow cytometry in measuring activated caspases in human spermatozoa
Date: Jun 8, 2009
Author:
Staining of spermatozoa with fluorescein-Val-Ala-Asp-fluoromethylketone using fluorescence microscopy or flow cytometry to score activated caspases was compared in order to adopt the most accurate method for detection in human frozen-thawed testicular, epididymal and ejaculated spermatozoa.
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Bi-curious cylinders
Date: Jun 1, 2009
Author: David Bradley
A team in the US has produced micrometre-wide discs and elongated rods from bi-coloured and multicolored compartments. The researchers used fluorescent emission studies and scanning electron microscopy to characterise their products.
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Using synchronous fluorescence spectroscopy and PCA to monitor dissolved organic matter dynamics in a glacier system
Date: May 11, 2009
Author:
Principal components analysis (PCA) of synchronous fluorescence spectra was used to detect and describe changes in the dissolved organic matter in meltwater from a glacier system in the Coast Mountains of northern British Columbia, Canada
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Berries beat UV-B
Date: May 1, 2009
Author: David Bradley
Using hairless laboratory rodents, researchers in Korea have demonstrated that the antioxidant ellagic acid, which is found in certain fruits and berries, can protect against damage to the skin caused by incident ultraviolet-B radiation. Their work suggests that the compound could help protect people to some extent from the skin-aging effects of the sun.
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Evaluation of the photodegradation of crystal violet upon light exposure by mass spectrometric and spectroscopic methods
Date: Apr 13, 2009
Author:
The degradation pathways of pure crystal violet in water and ethanol upon exposure to xenon light were compared by UV/VIS spectrophotometry and laser desorption ionization.
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Bond, Q, and controlled cleavage
Date: Apr 1, 2009
Author: David Bradley
US chemists have made an iron catalyst that can be used to rapidly break strong carbon-hydrogen bonds within molecules, up to one thousands times faster than other methods. The research could solve one of the great chemical challenges.
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In vivo fluorescence imaging: a personal perspective
Date: Mar 9, 2009
Author:
In this review of in vivo fluorescence imaging with NIR light, the use of polymersomes for the incorporation and delivery of large numbers of highly emissive oligo (porphyrin)-based, organic NIRFs is discussed.
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White light microscope
Date: Mar 1, 2009
Author: David Bradley
Silver nanoparticles that can generate white light could improve microscopy in research into cancer and bone diseases according to a paper in the March issue of Nano Letters.
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A novel assay based on fluorescence microscopy and image processing for determining phenotypic distributions of rod-shaped bacteria
Date: Feb 9, 2009
Author:
A novel assay combining fluorescence microscopy and image processing has been developed to determine the phenotypic distributions of the overall cell population, the dividing cell subpopulation and the newborn cell subpopulation of rod-shaped bacteria.
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