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Obscure crystals: Extracting extra from X-ray data

Date: May 1, 2017

Author: David Bradley

A new method allows scientists to extract previously hidden information from X-ray diffraction data on proteins and other biological molecules. The technique allows the difference between a target with and without its substrate to be more precisely refined in drug discovery.

Read More thumbnail image: Obscure crystals Extracting extra from X-ray data

NMR reveals entropy redistribution: Bacterial proteins

Date: May 1, 2017

Author: David Bradley

Research carried out at the of the METACyt Biomolecular NMR Laboratory at IU Bloomington at Indiana University has revealed evidence that tiny changes in atomic movements in bacterial proteins can play a big role in how these microorganisms function and evolve.

Read More thumbnail image: NMR reveals entropy redistribution Bacterial proteins

Mushrooms on the defensive: UV enzyme clues

Date: May 1, 2017

Author: David Bradley

Ultraviolet-visible (UV/Vis) spectroscopy and various other techniques, including MALDI mass spectrometry and 1D adn 2D nuclear magnetic resonance (NMR) spectroscopy have been used to help explain how mushrooms get defensive.

Read More thumbnail image: Mushrooms on the defensive UV enzyme clues

Emission control: Infrared metamaterial

Date: May 1, 2017

Author: David Bradley

A meta material that can be tuned to emit different wavelengths of infrared light in a controlled manner having absorbed energy could be used to make a device for collecting and so using waste heat.

Read More thumbnail image: Emission control Infrared metamaterial

Accentuating the positive: fMRI investigation

Date: May 1, 2017

Author: David Bradley

Functional MRI has been used to measure the activity in different regions of the brain of subjects shown disturbing images of violence - war and accidents - in an investigation aimed at understanding how poor sleep in anxiety and depression might make it harder for people to see the world positively.

Read More thumbnail image: Accentuating the positive fMRI investigation

Journal Highlight: 3D printing from MRI Data: Harnessing strengths and minimizing weaknesses

Date: Apr 24, 2017

Author: spectroscopyNOW

The basics of 3D printing and the current strengths and weaknesses of printing from MRI data as they pertain to model accuracy have been reviewed, covering considerations in the design of MRI sequences for 3D printing.

Read More thumbnail image: Journal Highlight 3D printing from MRI Data Harnessing strengths and minimizing weaknesses

Journal Highlight: The oxidized thiol proteome in aging and cataractous mouse and human lens revealed by ICAT labeling

Date: Apr 24, 2017

Author: spectroscopyNOW

All of the disulfide-forming noncrystallin proteins associated with age-related cataractogenesis in mice and humans have been identified using ICAT labelling techniques.

Read More thumbnail image: Journal Highlight The oxidized thiol proteome in aging and cataractous mouse and human lens revealed by ICAT labeling

UHPLC and Chemometrics Tease out Teasel’s Active Compounds

Date: Apr 17, 2017

Author: Ryan De Vooght-Johnson

Dipsacus apser, or Japanese teasel, is commonly used in Chinese herbal medicine, often after rinsing with wine and then boiling with water. Researchers from Nanjing University of Chinese Medicine compared the differences between the composition of the raw herb and wine-processed material, using UHPLC-MS/MS and the chemometric methods PCA, HCA and PLS-DA.

Read More thumbnail image: UHPLC and Chemometrics Tease out Teasels Active Compounds

Journal Highlight: UV/Vis spectroscopy combined with chemometrics for monitoring solid-state fermentation with Rhizopus microsporus var. oligosporus

Date: Apr 17, 2017

Author: spectroscopyNOW

Partial least squares and artificial neural network models have been presented for inferring protease and amylase activities from UV–vis spectra of samples removed during solid-state fermentation using Rhizopus microsporus var. oligosporus.

Read More thumbnail image: Journal Highlight UVVis spectroscopy combined with chemometrics for monitoring solid-state fermentation with Rhizopus microsporus var_ oligosporus

Cellular enzyme-substrate complexes: Probes for mass spectrometric detection

Date: Apr 15, 2017

Author: Steve Down

Enzyme complexes with other compounds that form in cells can be observed directly by mass spectrometry following a novel method developed in the US, in which an isotope-labelled probe is used to create a stable three-way complex with the enzyme and the substrate.

Read More thumbnail image: Cellular enzyme-substrate complexes Probes for mass spectrometric detection
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