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Evaluation of Δ 9 ‐tetrahydrocannabinol detection using DrugWipe5S ® screening and oral fluid quantification after Quantisal™ collection for roadside drug detection via a controlled study with chronic cannabis users

Published: Apr 21, 2014

Author(s): Sarah M. R. Wille, Vincent Di Fazio, Stefan W. Toennes, Janelle H. P. Wel, Johannes G. Ramaekers, Nele Samyn

Journal: Drug Testing and Analysis

Oral fluid (OF) is potentially useful to detect driving under the influence of drugs because of its ease of sampling. While cannabis is the most prevalent drug in Europe, sensitivity issues for Δ9‐tetrahydrocannabinol (THC) screening and problems during OF collection are observed. The ability of a recently improved OF screening device – the DrugWipe5S®, to detect recent THC use in chronic cannabis smokers, was studied. Ten subjects participated in a...

Read More thumbnail image: Evaluation of  9 tetrahydrocannabinol detection using DrugWipe5S  screening and oral fluid quantification after Quantisal collection for roadside drug detection via a controlled study with chronic cannabis users

Signal enhancement of glucuronide conjugates in LC‐MS/MS by derivatization with the phosphonium propylamine cation tris(trimethoxyphenyl) phosphonium propylamine, for forensic purposes

Published: Apr 21, 2014

Author(s): Sophie C. Turfus, John M. Halket, Mark C. Parkin, David A. Cowan, Robin A. Braithwaite, Andrew T. Kicman

Journal: Drug Testing and Analysis

Although chemical derivatization for signal enhancement in drug testing is most often associated with gas chromatography, it also has the potential to improve the detection of analytes poorly ionized by atmospheric pressure ionization techniques, such as electrospray ionization used in liquid chromatography‐mass spectrometry. A number of acidic compounds, namely drug glucuronides (e.g. conjugates of temazepam, oxazepam, lorazepam,...

Read More thumbnail image: Signal enhancement of glucuronide conjugates in LCMSMS by derivatization with the phosphonium propylamine cation tristrimethoxyphenyl phosphonium propylamine for forensic purposes

Prestaining of glycoproteins in sodium dodecyl sulfate polyacrylamide gels by dansylhydrazine

Published: Apr 19, 2014

Author(s): Yang Wang, Xuan Zhou, Qing Yu, Yuanmeng Duan, Binbin Huang, Guoying Hong, Ayi Zhou, Litai Jin

Journal: PROTEOMICS

A new fluorescent prestaining method for gel‐separated glycoproteins in 1D and 2D SDS‐PAGE was developed by using dansylhydrazine in this study. The prestained gels could be easily imaged after electrophoresis without any time‐consuming steps needed for poststains. As low as 4–8 ng glycoproteins (transferrin, α1‐acid glycoprotein) could be selectively detected, which is comparable to that of Pro‐Q Emerald 488, one of the most commonly...

Read More thumbnail image: Prestaining of glycoproteins in sodium dodecyl sulfate polyacrylamide gels by dansylhydrazine

Simultaneous determination of pharmaceuticals and some of their metabolites in wastewaters by high performance liquid chromatography with tandem mass spectrometry

Published: Apr 19, 2014

Author(s): Martina Periša, Sandra Babić

Journal: Journal of Separation Science

The present work describes the development of a sensitive and reliable analytical method based on solid‐phase extraction followed by analysis using liquid chromatography with tandem mass spectrometry for the simultaneous determination of pharmaceuticals from antibiotics (fluoroquinolones, sulfonamides, and their N4‐acetyl metabolites, and trimethoprim as sulfonamides synergist) and anthelmintics groups. SPE was optimized using different ...

Read More thumbnail image: Simultaneous determination of pharmaceuticals and some of their metabolites in wastewaters by high performance liquid chromatography with tandem mass spectrometry

Fitting formula for the injection volume of a gas chromatograph for radio‐xenon sampling in the lower troposphere

Published: Apr 19, 2014

Author(s): Liu Shu‐jiang, Chen Zhan‐ying, Wang Shi‐lian, Chang Yin‐zhong, Li Qi, Fan Yuan‐qing, Zhao Yun‐gang, Jia Huai‐mao, Zhang Xin‐jun, Wang Jun

Journal: Journal of Separation Science

GC is usually used for xenon concentration and radon removal in the International Monitoring System of the Comprehensive Nuclear‐Test‐Ban Treaty. In a gas chromatograph, the injection volume is defined to calculate the column capacity. In this paper, the injection volume was investigated and a fitting formula for the injection volume was derived and discussed subsequently. As a consequence, the xenon injection volume exponentially...

Read More thumbnail image: Fitting formula for the injection volume of a gas chromatograph for radioxenon sampling in the lower troposphere

Extraction optimization by response surface methodology: P urification and characterization of phytosterol from sugarcane ( Saccharum officinarum L .) rind

Published: Apr 19, 2014

Author(s): Simin Feng, Zisheng Luo, Zhou Zhong, Lei Jiang, Kaichen Tang

Journal: Journal of Separation Science

A green, simple, and effective method for the extraction of sugarcane lipids from sugarcane rind was investigated by response surface methodology. The optimum conditions of technological progress obtained through response surface methodology were as follows: liquid‐to‐solid ratio 7.94: 1 mL/g, extraction temperature 50°C and extraction time 5.98 h. The practical sugarcane lipids extraction yield was 6.55 ± 0.28%, which was in good...

Read More thumbnail image: Extraction optimization by response surface methodology P urification and characterization of phytosterol from sugarcane  Saccharum officinarum L _ rind

Simultaneous determination of metoprolol and its metabolites, α‐hydroxymetoprolol and O ‐desmethylmetoprolol, in human plasma by liquid chromatography with tandem mass spectrometry: A pplication to the pharmacokinetics of metopr

Published: Apr 19, 2014

Author(s): Soo Hyeon Bae, Joeng Kee Lee, Doo‐Yeoun Cho, Soo Kyung Bae

Journal: Journal of Separation Science

A rapid and simple LC with MS/MS method for the simultaneous determination of metoprolol and its two CYP2D6‐derived metabolites, α‐hydroxy‐ and O‐desmethylmetoprolol, in human plasma was established. Metoprolol (MET), its two metabolites, and the internal standard chlorpropamide were extracted from plasma (50 μL) using ethyl acetate. Chromatographic separation was performed on a Luna CN column with an isocratic mobile phase consisting of...

Read More thumbnail image: Simultaneous determination of metoprolol and its metabolites hydroxymetoprolol and O desmethylmetoprolol in human plasma by liquid chromatography with tandem mass spectrometry A pplication to the pharmacokinetics of metopr

Study of the retention behavior of small polar molecules on different types of stationary phases used in hydrophilic interaction liquid chromatography

Published: Apr 19, 2014

Author(s): Hana Vlčková, Kateřina Ježková, Kateřina Štětková, Helena Tomšíková, Petr Solich, Lucie Nováková

Journal: Journal of Separation Science

The retention behavior of a large group of analytes (35) with varied properties (pKa and logP) was studied on eight hydrophilic interaction LC columns with different surfaces, stationary phase chemistries, and types of particles. The acetonitrile content (5–95%), buffer concentration (0.5–200 mM), and pH of the mobile phase (3.8 and 6.8) were evaluated for their effects on the retention behavior. The type of stationary phase had a significant...

Read More thumbnail image: Study of the retention behavior of small polar molecules on different types of stationary phases used in hydrophilic interaction liquid chromatography

Artifacts to avoid while taking advantage of top‐down mass spectrometry based detection of protein S‐thiolation

Published: Apr 17, 2014

Author(s): Jared R. Auclair, Joseph P. Salisbury, Joshua L. Johnson, Gregory A. Petsko, Dagmar Ringe, Daryl A. Bosco, Nathalie Y. R. Agar, Sandro Santagata, Heather D. Durham, Jeffrey N. Agar

Journal: PROTEOMICS

Bottom‐up MS studies typically employ a reduction and alkylation step that eliminates a class of PTM, S‐thiolation. Given that molecular oxygen can mediate S‐thiolation from reduced thiols, which are abundant in the reducing intracellular milieu, we investigated the possibility that some S‐thiolation modifications are artifacts of protein preparation. Cu/Zn‐superoxide dismutase (SOD1) was chosen for this case study as it has a reactive...

Read More thumbnail image: Artifacts to avoid while taking advantage of topdown mass spectrometry based detection of protein Sthiolation

iTRAQ analysis of colorectal cancer cell lines suggests Drebrin (DBN1) is overexpressed during liver metastasis

Published: Apr 17, 2014

Author(s): Qifeng Lin, Hwee Tong Tan, Teck Kwang Lim, Avery Khoo, Kiat Hon Lim, Maxey C. M. Chung

Journal: PROTEOMICS

Colorectal cancer is currently the third in cancer incidence worldwide and the fourth most common cause of cancer deaths. Mortality in colorectal cancer is often ascribed to liver metastasis. In an effort to elucidate the proteins involved in colorectal cancer liver metastasis, we compared the proteome profiles of the human colon adenocarcinoma cell line HCT‐116 with its metastatic derivative E1, using the iTRAQ labelling technology,...

Read More thumbnail image: iTRAQ analysis of colorectal cancer cell lines suggests Drebrin DBN1 is overexpressed during liver metastasis

Comparative proteome analysis of tracheal tissues in response to infectious bronchitis coronavirus, Newcastle disease virus, and avian influenza virus H9 subtype virus infection

Published: Apr 17, 2014

Author(s): Junfeng Sun, Zongxi Han, Yuhao Shao, Zhongzan Cao, Xiangang Kong, Shengwang Liu

Journal: PROTEOMICS

Infectious bronchitis coronavirus (IBV), Newcastle disease virus (NDV), and avian influenza virus (AIV) H9 subtype are major pathogens of chickens causing serious respiratory tract disease and heavy economic losses. To better understand the replication features of these viruses in their target organs and molecular pathogenesis of these different viruses, comparative proteomic analysis was performed to investigate the proteome changes of...

Read More thumbnail image: Comparative proteome analysis of tracheal tissues in response to infectious bronchitis coronavirus Newcastle disease virus and avian influenza virus H9 subtype virus infection

MR‐monitored focused ultrasound using the acoustic‐coupling water bath as an intrinsic high‐mode dielectric resonator

Published: Apr 16, 2014

Author(s): A. G. Webb

Journal: NMR in Biomedicine

The conventional set‐up for MR‐monitored focused ultrasound surgery includes a piezoelectric transducer and an acoustic‐coupling water bath integrated into the MR patient table; a large surface RF coil is placed close to the patient or, alternatively, the body coil is used as the MR receiver. Potential disadvantages of this approach are that the body coil has low sensitivity because of its low filling factor and the local RF coil can...

Read More thumbnail image: MRmonitored focused ultrasound using the acousticcoupling water bath as an intrinsic highmode dielectric resonator

Functional assessment of the mouse heart by MRI with a 1‐min acquisition

Published: Apr 16, 2014

Author(s): Guido Buonincontri, Carmen Methner, Thomas Krieg, T. Adrian Carpenter, Stephen J. Sawiak

Journal: NMR in Biomedicine

In vivo assessment of heart function in mice is important for basic and translational research in cardiology. MRI is an accurate tool for the investigation of the anatomy and function in the preclinical setting; however, the long scan duration limits its usage. We aimed to reduce the acquisition time of cine MRI to 1 min. We employed spatiotemporal compressed sensing and parallel imaging to accelerate retrospectively gated cine MRI. We...

Read More thumbnail image: Functional assessment of the mouse heart by MRI with a 1min acquisition

Quantitative blood oxygenation level‐dependent (BOLD) response of the left ventricular myocardium to hyperoxic respiratory challenge at 1.5 and 3.0 T

Published: Apr 16, 2014

Author(s): Sebastian Winklhofer, Shila Pazahr, Robert Manka, Hatem Alkadhi, Andreas Boss, Paul Stolzmann

Journal: NMR in Biomedicine

The aim of this study was to quantify the response of the myocardial transverse relaxation times (ΔT2*) to hyperoxic respiratory challenge (HRC) at different field strengths in an intra‐individual comparison of healthy volunteers and in a patient with coronary artery disease. Blood oxygenation level‐dependent (BOLD) cardiovascular MR (CMR) data were acquired in 10 healthy volunteers (five women, five men; mean age, 29 ± 3 years; range, 22–35 years)...

Read More thumbnail image: Quantitative blood oxygenation leveldependent BOLD response of the left ventricular myocardium to hyperoxic respiratory challenge at 1_5 and 3_0T

Microwave‐assisted extraction coupled with countercurrent chromatography for the rapid preparation of flavonoids from Scutellaria barbata D. Don

Published: Apr 16, 2014

Author(s): Chong Ruan, Xiao‐hua Xiao, Gong‐ke Li

Journal: Journal of Separation Science

As a famous Chinese herb having good inhibitory effects on numerous human cancers both in vitro and in vivo, Scutellaria barbata D. Don attracts extensive attention worldwide. In this work, four flavonoids named scutellarin, baicalin, luteolin, and apigenin were simply and rapidly prepared from S. barbata by microwave‐assisted extraction coupled to countercurrent chromatography. Extraction conditions including irradiation time, extraction...

Read More thumbnail image: Microwaveassisted extraction coupled with countercurrent chromatography for the rapid preparation of flavonoids from Scutellaria barbata D_ Don

Supercoiled plasmid DNA purification by integrating membrane technology with a monolithic chromatography

Published: Apr 16, 2014

Author(s): Catherine Nunes, Ângela Sousa, José C. Nunes, António M. Morão, Fani Sousa, João A. Queiroz

Journal: Journal of Separation Science

The present study describes the integration of membrane technology with monolithic chromatography to obtain plasmid DNA with high quality. Isolation and clarification of plasmid DNA lysate were first conducted by a microfiltration step, by using a hydrophilic nylon microfiltration membrane, avoiding the need of centrifugation. For the total elimination of the remaining impurities, a suitable purification step is required. Monolithic...

Read More thumbnail image: Supercoiled plasmid DNA purification by integrating membrane technology with a monolithic chromatography

The role of metallothionein interactions with other proteins

Published: Apr 16, 2014

Author(s): Marta Zalewska, Jagoda Trefon, Halina Milnerowicz

Journal: PROTEOMICS

Metallothionein (MT) is a protein involved in numerous key processes, and the most important include zinc ion homeostasis, detoxification of heavy metals, and protection against oxidative stress. MT by interaction with other proteins fulfills its function, resulting in different effects in the body. Interaction of MT with ferritin, which causes a redox reaction, resulting in the reduction of Fe3+ stored in ferritin and a release of...

Read More thumbnail image: The role of metallothionein interactions with other proteins

Identification of proteins associated with ion homeostasis and salt tolerance in barley

Published: Apr 16, 2014

Author(s): Dezhi Wu, Qiufang Shen, Long Qiu, Yong Han, Linzheng Ye, Zahra Jabeen, Qingyao Shu, Guoping Zhang

Journal: PROTEOMICS

Identification and characterization of proteins involved in salt tolerance are imperative for revealing its genetic mechanisms. In this study, ionic and proteomic responses of a Tibetan wild barley XZ16 and a well‐known salt‐tolerant barley cv. CM72 were analyzed using inductively coupled plasma‐optical emission spectrometer, 2DE, and MALDI‐TOF/TOF MS techniques to determine salt‐induced differences in element and protein profiles between...

Read More thumbnail image: Identification of proteins associated with ion homeostasis and salt tolerance in barley

High resolution two‐dimensional electrophoresis of native proteins

Published: Apr 16, 2014

Author(s): Florian Weiland, Carla M. Zammit, Frank Reith, Peter Hoffmann

Journal: ELECTROPHORESIS

Blue native PAGE (BN‐PAGE) is a powerful method to separate protein complexes while preserving their native state. However, the resolution of the method is limited as complexes with similar molecular masses cannot be resolved. Here we describe native 2DE using immobilized pH‐gradients in combination with BN‐PAGE to resolve protein complexes by their pI and molecular mass. This method enables electrophoretic separation of proteins between pI 3 and 10...

Read More thumbnail image: High resolution twodimensional electrophoresis of native proteins

The application of targeted mass spectrometry‐based strategies to the detection and localization of post‐translational modifications

Published: Apr 15, 2014

Author(s): Navin Chicooree, Richard D. Unwin, John R. Griffiths

Journal: Mass Spectrometry Reviews

This review describes some of the more interesting and imaginative ways in which mass spectrometry has been utilized to study a number of important post‐translational modifications over the past two decades; from circa 1990 to 2013. A diverse range of modifications is covered, including citrullination, sulfation, hydroxylation and sumoylation. A summary of the biological role of each modification described, along...

Read More thumbnail image: The application of targeted mass spectrometrybased strategies to the detection and localization of posttranslational modifications
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