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Separation of HIV‐1 gag virus‐like particles from vesicular particles impurities by hydroxyl‐functionalized monoliths

Published: Jan 20, 2017

Author(s): Petra Steppert, Daniel Burgstaller, Miriam Klausberger, Petra Kramberger, Andres Tover, Eva Berger, Katharina Nöbauer, Ebrahim Razzazi‐Fazeli, Alois Jungbauer

Journal: Journal of Separation Science

The downstream processing of enveloped virus‐like particles is very challenging because of the biophysical and structural similarity between correctly assembled particles and contaminating vesicular particles present in the feedstock. We used hydroxyl‐functionalized polymethacrylate monoliths, providing hydrophobic and electrostatic binding contributions, for the purification of HIV‐1 gag virus‐like particles. The clarified culture...

Read More thumbnail image: Separation of HIV1 gag viruslike particles from vesicular particles impurities by hydroxylfunctionalized monoliths

Polydopamine‐assisted immobilization of zeolitic imidazolate framework‐8 for open‐tubular capillary electrochromatography

Published: Jan 20, 2017

Author(s): Yilin Li, Tao Bao, Zilin Chen

Journal: Journal of Separation Science

In this work, we developed a capillary column modified with zeolitic imidazolate framework‐8 as a novel stationary phase for open‐tubular capillary electrochromatography. To immobilize zeolitic imidazolate framework‐8 onto the inner surface of silica capillary, a bio‐inspired polydopamine functionalization was used to functionalize the capillary surface with polydopamine. First, a polydopamine layer was assembled inside the capillary....

Read More thumbnail image: Polydopamineassisted immobilization of zeolitic imidazolate framework8 for opentubular capillary electrochromatography

Analysis of terpenes and turpentines using gas chromatography with vacuum ultraviolet detection

Published: Jan 20, 2017

Author(s): Changling Qiu, Jonathan Smuts, Kevin A. Schug

Journal: Journal of Separation Science

The separation and identification of natural mixtures of terpenes is challenging and laborious. A gas chromatographic method based on vacuum ultraviolet spectroscopic detection, which is characterized by full‐scan absorption in the range of 125–240 nm, was developed and applied to analyze terpenes. In this study, the vacuum ultraviolet absorption spectra of 41 different standard terpenes were investigated and compared. The spectra were...

Read More thumbnail image: Analysis of terpenes and turpentines using gas chromatography with vacuum ultraviolet detection

Hollow fiber stir bar sorptive extraction for determination of phthalic acid esters in environmental and biological matrices

Published: Jan 20, 2017

Author(s): Jia Li, Yan–Bin Wang, Qiong Su, Shang Wu, Lan Wu

Journal: Journal of Separation Science

The purpose of this paper is to introduce a novel hollow fiber stir bar sorptive extraction for collecting and determining of phthalic acid esters in environmental and biological matrices. Shell–core ZrO2/SiO2 composite microspheres and porous C18 silica microspheres were compared as the sorbents, which were loaded in the lumen of a microporous hollow fiber membrane. A thin stainless‐steel wire was also inside of the hollow fiber membrane acting as...

Read More thumbnail image: Hollow fiber stir bar sorptive extraction for determination of phthalic acid esters in environmental and biological matrices

Determination of rabeprazole enantiomers in dog plasma by supercritical fluid chromatography tandem mass spectrometry and its application to a pharmacokinetic study

Published: Jan 20, 2017

Author(s): Chong Su, Hong Yang, Xiangjun Meng, J. Paul Fawcett, Jianming Cao, Yan Yang, Jingkai Gu

Journal: Journal of Separation Science

Rabeprazole is a novel benzimidazole proton pump inhibitor used for the treatment of gastrointestinal disorders. It is a chiral molecule that gives rise to the possibility of stereoselective pharmacokinetics. To investigate this phenomenon, a rapid and sensitive chiral assay based on supercritical fluid chromatography tandem mass spectrometry was developed and applied to the determination of (R)‐rabeprazole and (S)‐rabeprazole in dog...

Read More thumbnail image: Determination of rabeprazole enantiomers in dog plasma by supercritical fluid chromatography tandem mass spectrometry and its application to a pharmacokinetic study

Novel approach to determine ghrelin analogs by liquid chromatography with mass spectrometry using a monolithic column

Published: Jan 20, 2017

Author(s): Jana Zemenova, David Sykora, Hana Adamkova, Lenka Maletinska, Tomas Elbert, Ales Marek, Miroslava Blechova

Journal: Journal of Separation Science

In our project, ghrelin analogs possessing enhanced stability and potential to significantly increase food intake were used. Three newly synthesized ghrelin analogs with fatty acid residues consisting of 8, 10, and 14 carbon atoms were studied. The main goal of this work was to develop a suitable analytical method for the determination of the stability of the novel ghrelin analogs in plasma. An appropriate liquid chromatography‐mass...

Read More thumbnail image: Novel approach to determine ghrelin analogs by liquid chromatography with mass spectrometry using a monolithic column

Combined X‐ray diffraction and alpha particle X‐ray spectrometer analysis of geologic materials

Published: Jan 20, 2017

Author(s): Glynis M. Perrett, John A. Maxwell, John L. Campbell

Journal: X-Ray Spectrometry

The shallow interrogation depth of the lightest elements (Na, Mg, Al, and Si) detected by the particle‐induced X‐ray emission branch of the Curiosity Rover's alpha particle X‐ray spectrometer suggests that the X‐rays of these elements very likely emerge from a single mineral grain. This reality violates the assumption of atomic homogeneity at the micron scale made in both existing spectrum‐reduction approaches for the alpha particle X‐ray...

Read More thumbnail image: Combined Xray diffraction and alpha particle Xray spectrometer analysis of geologic materials

A comparative study on the total reflection X‐ray fluorescence determination of low Z elements using X‐ray tube and synchrotron radiation as excitation sources

Published: Jan 20, 2017

Author(s): K. Sanyal, B. Kanrar, N. L. Misra, M. Czyzycki, A. Migliori, A. G. Karydas

Journal: X-Ray Spectrometry

Detailed total reflection X‐ray fluorescence (TXRF) studies for the detection and quantification of low atomic number elements were carried out by using a laboratory dual source TXRF spectrometer equipped with a vacuum chamber and at the International Atomic Energy Agency multi‐purpose end‐station facility, operated at the XRF beamline of Elettra Sincrotrone Trieste, Italy. Multi‐elemental standard aqueous solutions of low Z elements (F,...

Read More thumbnail image: A comparative study on the total reflection Xray fluorescence determination of low Z elements using Xray tube and synchrotron radiation as excitation sources

Surface properties of metal alloys used in aviation after plasma treatment

Published: Jan 20, 2017

Author(s): Konrad Terpilowski, Diana Rymuszka, Lucyna Hołysz, Marek Ilnicki

Journal: Surface and Interface Analysis

The metal alloys: aluminum AMS 4037, 4041, 4049, carbon steel AMS 5045, titanium alloy AMS 4911 and cadmium steel used in aviation were treated by air and argon plasmas. For their activation, low pressure plasma was used. Surface treatment caused a significant increase of wettability due to the increasing surface roughness and introduction of polar functional groups onto the surface. Changes of surface properties are reflected by the...

Read More thumbnail image: Surface properties of metal alloys used in aviation after plasma treatment

Application of a liquid chromatography–tandem mass spectrometry method to the pharmacokinetics, tissue distribution and excretion in the study of anemoside B4, a novel antiviral agent candidate, in rats

Published: Jan 20, 2017

Author(s): Mingyu He, Hui Ouyang, Mingzhen He, Ting Tan, Junmao Li, Xiaoyong Zhang, Jia Jia, Yulin Feng, Shilin Yang

Journal: Biomedical Chromatography

Abstract A simple, sensitive and reliable LC–MS/MS method was developed and validated for the quantification of anemoside B4, a potential antiviral constituent isolated from Pulsatilla chinensis in rat plasma, tissue, bile, urine and feces. All biological samples were prepared by protein precipitation method, and ginsenoside‐Rg1 was chosen as the internal standard (IS). The analyte and IS were separated using a C18 column (2.1 × 50 mm, 1.8 μm)...

Read More thumbnail image: Application of a liquid chromatographytandem mass spectrometry method to the pharmacokinetics tissue distribution and excretion in the study of anemoside B4 a novel antiviral agent candidate in rats

Interface of ZnS single sheet and substrates: a first‐principles study

Published: Jan 20, 2017

Author(s): Wen Yu, Xiao Wang, Chuanguo Li, Lixia Xiao

Journal: Surface and Interface Analysis

Two‐dimensional materials have aroused great interests because of their unique properties not seen in the bulk counterparts. The interface of the ZnS single sheet and substrates are studied in this paper. Different from isolated ZnS single sheet, here in this study, the ZnS single sheet has a remarkable corrugation feature because of the interaction between the ZnS single sheet and the substrate. The top‐site Zn means an attractive...

Read More thumbnail image: Interface of ZnS single sheet and substrates a firstprinciples study

Application of response surface methodology for optimization of trace amount of diazinon preconcentration in natural waters and biological samples by carbon mesoporous CMK‐3

Published: Jan 19, 2017

Author(s): Somayeh Beirami, Hadi Rahimzadeh Barzoki, Nader Bahramifar

Journal: Biomedical Chromatography

Abstract Preconcentration of trace amounts of diazinon by carbon mesoporous CMK‐3 in water and biological samples and measurement by high‐performance liquid chromatography were investigated. CMK‐3 was prepared using hexagonal SBA‐15 as the template. The synthesized materials were characterized by X‐Ray diffraction (XRD), Fourier transform infrared spectroscopy, Brunaur–Emmet–Teller, transmission electron microscopy and Boehm...

Read More thumbnail image: Application of response surface methodology for optimization of trace amount of diazinon preconcentration in natural waters and biological samples by carbon mesoporous CMK3

Solvent‐mediated nonelectrostatic ion–ion interactions predicting anomalies in electrophoresis

Published: Jan 19, 2017

Author(s): Prakash Goswami, Jayabrata Dhar, Uddipta Ghosh, Suman Chakraborty

Journal: ELECTROPHORESIS

We study the effects of solvent‐mediated nonelectrostatic ion–ion interactions on electrophoretic mobility of a charged spherical particle. To this end, we consider the case of low surface electrostatic potential resulting in the linearization of the governing equations, which enables us to deduce a closed‐form analytical solution to the electrophoretic mobility. We subsequently compare our results to the standard model using Henry's...

Read More thumbnail image: Solventmediated nonelectrostatic ionion interactions predicting anomalies in electrophoresis

DT2 fit of a high‐resolution EDS PIXE Yb 2 O 3 spectrum

Published: Jan 19, 2017

Author(s): M. A. Reis, P. C. Chaves

Journal: X-Ray Spectrometry

Analysis of high‐resolution EDS particle X‐ray emission spectra is typically more complex than standard Si(Li) detector spectra analysis because of the need to carefully account for contributions from minor and satellite transitions. In this work, a pure Yb2O3 sample was irradiated at the high‐resolution high‐energy‐particle X‐ray emission setup of C2TN, and simultaneous CdTe and X‐ray microcalorimeter spectrometer spectra were collected covering...

Read More thumbnail image: DT2 fit of a highresolution EDS PIXE Yb 2 O 3 spectrum

Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

Published: Jan 17, 2017

Author(s): Jinmao You, Di Wu, Mei Zhao, Guoliang Li, Peiwei Gong, Yueyue Wu, Yu Guo, Guang Chen, Xianen Zhao, Zhiwei Sun, Lian Xia, Yongning Wu

Journal: Biomedical Chromatography

Abstract Triterpenic acids are widely distributed in many fruits and are known for their medicinal benefits. The study of bioavailability has been an important task for a better understanding of the triterpenic acids. Although many methods based on fluorescence labeling for triterpenic acid determination have been established, these reported methods needed anhydrous conditions, which are not suitable for the convenient study of...

Read More thumbnail image: Development of a facile and sensitive HPLCFLD method via fluorescence labeling for triterpenic acid bioavailability investigation

Exploring STR signal in the single‐ and multicopy number regimes: Deductions from an in silico model of the entire DNA laboratory process

Published: Jan 17, 2017

Author(s): Ken R. Duffy, Neil Gurram, Kelsey C. Peters, Genevieve Wellner, Catherine M. Grgicak

Journal: ELECTROPHORESIS

Short tandem repeat (STR) profiling from DNA samples has long been the bedrock of human identification. The laboratory process is composed of multiple procedures that include quantification, sample dilution, PCR, electrophoresis, and fragment analysis. The end product is a short tandem repeat electropherogram comprised of signal from allele, artifacts, and instrument noise. In order to optimize or alter laboratory protocols, a large...

Read More thumbnail image: Exploring STR signal in the single and multicopy number regimes Deductions from an in silico model of the entire DNA laboratory process

Protein‐phosphotyrosine proteome profiling by superbinder‐SH2 domain affinity purification mass spectrometry, sSH2‐AP‐MS

Published: Jan 17, 2017

Author(s): Jiefei Tong, Biyin Cao, Gregory D. Martyn, Jonathan R. Krieger, Paul Taylor, Bradley Yates, Sachdev S. Sidhu, Shawn S. C. Li, Xinliang Mao, Michael F. Moran

Journal: PROTEOMICS

Recently, “superbinder” SH2 domain variants with three amino acid substitutions (sSH2) were reported to have 100‐fold or greater affinity for protein‐phosphotyrosine (pY) than natural SH2 domains. Here we report a protocol in which His‐tagged Src sSH2 efficiently captures pY‐peptides from protease‐digested HeLa cell total protein extracts. Affinity purification of pY‐peptides by this method shows little bias for pY‐proximal amino acid...

Read More thumbnail image: Proteinphosphotyrosine proteome profiling by superbinderSH2 domain affinity purification mass spectrometry sSH2APMS

Metabolomics‐based approach for ranking the candidate structures of unidentified peaks in capillary electrophoresis time‐of‐flight mass spectrometry

Published: Jan 17, 2017

Author(s): Hiroyuki Yamamoto, Kazunori Sasaki

Journal: ELECTROPHORESIS

One of the technical challenges encountered during metabolomics research is determining the chemical structures of unidentified peaks. We have developed a metabolomics‐based chemoinformatics approach for ranking the candidate structures of unidentified peaks. Our approach uses information about the known metabolites detected in samples containing unidentified peaks and involves three discrete steps. The first step involves identifying...

Read More thumbnail image: Metabolomicsbased approach for ranking the candidate structures of unidentified peaks in capillary electrophoresis timeofflight mass spectrometry

β‐cyclodextrin‐ionic liquid polymer based dynamically coating for simultaneous determination of tetracyclines by capillary electrophoresis

Published: Jan 17, 2017

Author(s): Chunyan Zhou, Jingjing Deng, Guoyue Shi, Tianshu Zhou

Journal: ELECTROPHORESIS

Tetracyclines are a group of broad spectrum antibiotics widely used in animal husbandry to prevent and treat diseases. However, the improper use of tetracyclines may result in the presence of their residues in animal tissues or waste. Recently, great attention has been drawn towards the green solvents ionic liquids. Ionic liquids have been employed as a coating material to modify the electroosmotic flow in capillary electrophoresis. In...

Read More thumbnail image: cyclodextrinionic liquid polymer based dynamically coating for simultaneous determination of tetracyclines by capillary electrophoresis

Steady‐state protein focusing in carrier ampholyte based isoelectric focusing: Part I—Analytical solution

Published: Jan 17, 2017

Author(s): Jaesool Shim, Kisoo Yoo, Prashanta Dutta

Journal: ELECTROPHORESIS

The determination of an analytical solution to find the steady‐state protein concentration distribution in IEF is very challenging due to the nonlinear coupling between mass and charge conservation equations. In this study, approximate analytical solutions are obtained for steady‐state protein distribution in carrier ampholyte based IEF. Similar to the work of Svensson, the final concentration profile for proteins is assumed to be...

Read More thumbnail image: Steadystate protein focusing in carrier ampholyte based isoelectric focusing Part IAnalytical solution
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