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Small‐scale purification of butyrylcholinesterase from human plasma and implementation of a μLC‐UV/ESI MS/MS method to detect its organophosphorus adducts

Published: Mar 31, 2015

Author(s): Harald John, Felicitas Breyer, Christian Schmidt, Boris Mizaikoff, Franz Worek, Horst Thiermann

Journal: Drug Testing and Analysis

Human butyrylcholinesterase (hBChE) is a serine hydrolase (EC 3.1.1.8) present in all mammalian tissues and the bloodstream. Similar to acetylcholinesterase, the enzyme reacts with organophosphorus compounds (OP) like nerve agents or pesticides that cause enzyme inhibition (BChE adducts). These adducts represent valuable biomarkers for analytical verification of OP exposure. For establishment of these mass spectrometry based methods...

Read More thumbnail image: Smallscale purification of butyrylcholinesterase from human plasma and implementation of a LCUVESI MSMS method to detect its organophosphorus adducts

Discovery of a new caerulescent Psilocybe mushroom in Germany: Psilocybe germanica sp.nov.

Published: Mar 31, 2015

Author(s): Jochen Gartz, Georg Wiedemann

Journal: Drug Testing and Analysis

Read More thumbnail image: Discovery of a new caerulescent Psilocybe mushroom in Germany Psilocybe germanica sp_nov_

Cytotoxic mechanisms of panduratin A on A375 melanoma cells: A quantitative and temporal proteomics analysis

Published: Mar 30, 2015

Author(s): Siew‐Li Lai, Pooi‐Fong Wong, Teck‐Kwang Lim, Qingsong Lin, Mohd Rais Mustafa

Journal: PROTEOMICS

Melanoma is a lethal form of skin cancer with rising global incidence. However, limited treatment options are available for advanced melanoma and this is further compounded by the development of resistance toward existing drugs. Panduratin A (PA), a cyclohexanyl chalcone found in Boesenbergia rotunda, was investigated for its cytotoxic potentials against human malignant melanoma A375 cells. Our initial findings revealed that mitochondrion...

Read More thumbnail image: Cytotoxic mechanisms of panduratin A on A375 melanoma cells A quantitative and temporal proteomics analysis

Proteomic changes in the brain of the western painted turtle ( Chrysemys picta bellii ) during exposure to anoxia

Published: Mar 30, 2015

Author(s): Richard W. Smith, Phil Cash, David W. Hogg, Leslie T. Buck

Journal: PROTEOMICS

During anoxia, overall protein synthesis is almost undetectable in the brain of the western painted turtle. The aim of this investigation was to address the question of whether there are alterations to specific proteins by comparing the normoxic and anoxic brain proteomes. Reductions in creatine kinase, hexokinase, glyceraldehyde‐3‐phosphate dehydrogenase, and pyruvate kinase reflected the reduced production of adenosine triphosphate...

Read More thumbnail image: Proteomic changes in the brain of the western painted turtle  Chrysemys picta bellii  during exposure to anoxia

Bottom up proteomics data analysis strategies to explore protein modifications and genomic variants

Published: Mar 30, 2015

Author(s): Ana Sofia Carvalho, Deborah Penque, Rune Matthiesen

Journal: PROTEOMICS

The quest to understand biological systems requires further attention of the scientific community to the challenges faced in proteomics. In fact the complexity of the proteome reaches uncountable orders of magnitude. This means that significant technical and data‐analytic innovations will be needed for the full understanding of biology. Current state of art MS is probably our best choice for studying protein complexity and exploring new...

Read More thumbnail image: Bottom up proteomics data analysis strategies to explore protein modifications and genomic variants

Quantitative proteomic analysis of Giardia duodenalis assemblage A: A baseline for host, assemblage, and isolate variation

Published: Mar 30, 2015

Author(s): Samantha J. Emery, Ernest Lacey, Paul A. Haynes

Journal: PROTEOMICS

Giardia duodenalis is a gastrointestinal protozoan parasite of vertebrates and is a species complex comprised of eight assemblages, with the zoonotic assemblage A one of two subtypes infective for humans. With increasing genomic and transcriptomic data publicly available through the centralized giardiaDB.org, we have quantitatively analyzed the proteomes of eight G. duodenalis assemblage A strains (seven A1 and one A2) to provide a...

Read More thumbnail image: Quantitative proteomic analysis of Giardia duodenalis assemblage A A baseline for host assemblage and isolate variation

Measurement of distinctive features of cortical spreading depolarizations with different MRI contrasts

Published: Mar 27, 2015

Author(s): S. Umesh Rudrapatna, Arend M. Hamming, Marieke J. H. Wermer, Annette Toorn, Rick M. Dijkhuizen

Journal: NMR in Biomedicine

Growing clinical evidence suggests critical involvement of spreading depolarizations (SDs) in the pathophysiology of neurological disorders such as migraine and stroke. MRI provides powerful tools to detect and assess co‐occurring cerebral hemodynamic and cellular changes during SDs. This study reports the feasibility and advantages of two MRI scans, based on balanced steady‐state free precession (b‐SSFP) and diffusion‐weighted...

Read More thumbnail image: Measurement of distinctive features of cortical spreading depolarizations with different MRI contrasts

Three‐phase hollow‐fiber microextraction combined with ion‐pair high‐performance liquid chromatography for the simultaneous determination of five components of compound α‐ketoacid tablets in human urine

Published: Mar 26, 2015

Author(s): Zaifa Pan, Xiaoya Huang, Yuan Zhong, Lili Wang, Danhua Zhu, Lanjuan Li

Journal: Journal of Separation Science

The determination of α‐ketoacid concentration is demanded to evaluate the absorption and metabolic behavior of compound α‐ketoacid tablets taken by chronic kidney disease patients. To eliminate the interference of endogenous substance of urine and enrich the analytes, a three‐phase hollow‐fiber liquid‐phase microextraction combined with ion‐pair high‐performance liquid chromatography method was established for the determination of...

Read More thumbnail image: Threephase hollowfiber microextraction combined with ionpair highperformance liquid chromatography for the simultaneous determination of five components of compound ketoacid tablets in human urine

Dispersive solid–liquid phase microextraction based on nanomagnetic Preyssler heteropolyacid: A novel method for the preconcentration of nortriptyline

Published: Mar 26, 2015

Author(s): Zarrin Es'haghi, Sara Hooshmand

Journal: Journal of Separation Science

In this study, a new, simple, rapid, and efficient method combined with ultraviolet visible spectrophotometry and high‐performance liquid chromatography analysis was developed for the extraction and determination of nortriptyline. The tendency of the Preyssler tungsten heteropolyacid, H14[NaP5W30O110], immobilized on the surface of mesoporous nanomagnetite to adsorb the drug from the solution has been investigated. This method involves...

Read More thumbnail image: Dispersive solidliquid phase microextraction based on nanomagnetic Preyssler heteropolyacid A novel method for the preconcentration of nortriptyline

Development and validation of a generic liquid chromatographic method for the simultaneous determination of five commonly used antimalarial drugs: Application to pharmaceutical formulations and human plasma

Published: Mar 26, 2015

Author(s): Sai Sandeep Mannemala, Janaki Sankarachari Krishnan Nagarajan

Journal: Journal of Separation Science

A simple, sensitive, and rapid liquid chromatographic method was developed and validated using diode array detection for the determination of five commonly used antimalarial drugs in pharmaceutical formulations and in human plasma. Chromatographic separation of antimalarial drugs and internal standard (ibuprofen) was achieved on a C18 column with a mobile phase composed of 10 mM dipotassium orthophosphate at pH 3.0, methanol, and acetonitrile in a...

Read More thumbnail image: Development and validation of a generic liquid chromatographic method for the simultaneous determination of five commonly used antimalarial drugs Application to pharmaceutical formulations and human plasma

Rapid identification of efavirenz metabolites in rats and humans by ultra high performance liquid chromatography combined with quadrupole time‐of‐flight tandem mass spectrometry

Published: Mar 26, 2015

Author(s): Yachen Deng, Rong Liu, Ping Yang, Jianying Liang

Journal: Journal of Separation Science

An in vivo study of efavirenz metabolites in rats and human patients with ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry combined with MetabolitePilotMT software is reported for the first time. Considering the polarity differences between the metabolites, solid‐phase extraction and protein precipitation were both applied as a part of the sample preparation method. The structures of the...

Read More thumbnail image: Rapid identification of efavirenz metabolites in rats and humans by ultra high performance liquid chromatography combined with quadrupole timeofflight tandem mass spectrometry

Ultrasound‐assisted emulsification microextraction coupled with high‐performance liquid chromatography for the simultaneous determination of fragrance allergens in cosmetics and water

Published: Mar 26, 2015

Author(s): Jessica Pérez‐Outeiral, Esmeralda Millán, Rosa Garcia‐Arrona

Journal: Journal of Separation Science

A simple, inexpensive, and environmentally friendly method based on ultrasound‐assisted emulsification microextraction followed by solidification of floating organic drop and high‐performance liquid chromatography coupled to diode array detection was developed for the simultaneous determination of 18 potentially allergenic fragrance substances. Several parameters affecting the microextraction process were investigated in detail by the...

Read More thumbnail image: Ultrasoundassisted emulsification microextraction coupled with highperformance liquid chromatography for the simultaneous determination of fragrance allergens in cosmetics and water

iTRAQ proteomic analysis of N‐acetylmuramic acid mediated anti‐inflammatory capacity in LPS‐induced RAW 264.7 cells

Published: Mar 26, 2015

Author(s): Zhen Wu, Daodong Pan, Yuxing Guo, Xiaoqun Zeng, Yangying Sun

Journal: PROTEOMICS

Lactobacillus acidophilus probiotic bacteria have lasting beneficial health effects in the gastrointestinal tract, including protecting against pathogens, improving immunomodulation, and producing beneficial bacteria‐derived molecules. In lipopolysaccharide (LPS) induced RAW 264.7 cells treated with peptidoglycan or N‐acetylmuramic acid (NAM) from L. acidophilus, 390 differentially expressed proteins (8.76%) were identified by iTRAQ analysis,...

Read More thumbnail image: iTRAQ proteomic analysis of Nacetylmuramic acid mediated antiinflammatory capacity in LPSinduced RAW 264_7cells

Identification of nodule‐specific cysteine‐rich plant peptides in endosymbiotic bacteria

Published: Mar 26, 2015

Author(s): Hajnalka Durgo, Eva Klement, Eva Hunyadi‐Gulyas, Attila Szucs, Attila Kereszt, Katalin F. Medzihradszky, Eva Kondorosi

Journal: PROTEOMICS

The symbiosis of Medicago truncatula with Sinorhizobium meliloti or Sinorhizobium medicae soil bacteria results in the formation of root nodules where bacteria inside the plant cells are irreversibly converted to polyploid, nondividing nitrogen‐fixing bacteroids. Bacteroid differentiation is host‐controlled and the plant effectors are symbiosis‐specific secreted plant peptides. In the M. truncatula genome there are more than 600 symbiotic peptide...

Read More thumbnail image: Identification of nodulespecific cysteinerich plant peptides in endosymbiotic bacteria

Detection of GHRP‐2 and GHRP‐6 in urine samples from athletes

Published: Mar 25, 2015

Author(s): Holly D. Cox, Cole M. Hughes, Daniel Eichner

Journal: Drug Testing and Analysis

Read More thumbnail image: Detection of GHRP2 and GHRP6 in urine samples from athletes

Determining the authenticity of athlete urine in doping control by DNA analysis

Published: Mar 25, 2015

Author(s): Laurence Devesse, Denise Syndercombe Court, David Cowan

Journal: Drug Testing and Analysis

The integrity of urine samples collected from athletes for doping control is essential. The authenticity of samples may be contested, leading to the need for a robust sample identification method. DNA typing using short tandem repeats (STR) can be used for identification purposes, but its application to cellular DNA in urine has so far been limited. Here, a reliable and accurate method is reported for the successful identification of...

Read More thumbnail image: Determining the authenticity of athlete urine in doping control by DNA analysis

Predictive accuracy of single‐ and multi‐slice MRI for the estimation of total visceral adipose tissue in overweight to severely obese patients

Published: Mar 25, 2015

Author(s): Alexander Schaudinn, Nicolas Linder, Nikita Garnov, Felix Kerlikowsky, Matthias Blüher, Arne Dietrich, Tatjana Schütz, Thomas Karlas, Thomas Kahn, Harald Busse

Journal: NMR in Biomedicine

The quantification of visceral adipose tissue (VAT) is increasingly being considered for risk assessment and treatment monitoring in obese patients, but is generally time‐consuming. The goals of this work were to semi‐automatically segment and quantify VAT areas of MRI slices at previously proposed anatomical landmarks and to evaluate their predictive power for whole‐abdominal VAT volumes on a relatively large number of patients....

Read More thumbnail image: Predictive accuracy of single and multislice MRI for the estimation of total visceral adipose tissue in overweight to severely obese patients

Simultaneous imaging of 13 C metabolism and 1 H structure: technical considerations and potential applications

Published: Mar 25, 2015

Author(s): Jeremy W. Gordon, Sean B. Fain, David J. Niles, Kai D. Ludwig, Kevin M. Johnson, Eric T. Peterson

Journal: NMR in Biomedicine

Real‐time imaging of 13C metabolism in vivo has been enabled by recent advances in hyperpolarization. As a result of the inherently low natural abundance of endogenous 13C nuclei, hyperpolarized 13C images lack structural information that could be used to aid in motion detection and anatomical registration. Motion before or during the 13C acquisition can therefore result in artifacts and misregistration that may obscure measures of metabolism. In...

Read More thumbnail image: Simultaneous imaging of 13 C metabolism and 1 H structure technical considerations and potential applications

Ultra high performance liquid chromatography tandem mass spectrometry method for the simultaneous determination of multiple bioactive constituents in fruit extracts of Myristica fragrans and its marketed polyherbal formulations using a polarity switching technique

Published: Mar 25, 2015

Author(s): Renu Pandey, Koranappallil Bahuleyan Rameshkumar, Brijesh Kumar

Journal: Journal of Separation Science

Fruits of Myristica fragrans Houtt. are the source of two valuable spices: nutmeg and mace, traditionally used for its flavoring and medicinal properties and found as an ingredient in many marketed polyherbal formulations and food products. In this study, a sensitive and efficient ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry method was developed and validated for the rapid determination of...

Read More thumbnail image: Ultra high performance liquid chromatography tandem mass spectrometry method for the simultaneous determination of multiple bioactive constituents in fruit extracts of Myristica fragrans and its marketed polyherbal formulations using a polarity switching technique

Quality evaluation of Salvia miltiorrhiza Bge. by ultra high performance liquid chromatography with photodiode array detection and chemical fingerprinting coupled with chemometric analysis

Published: Mar 25, 2015

Author(s): Hongli Luo, Weijun Kong, Yichen Hu, Ping Chen, Xiaoru Wu, Li Wan, Meihua Yang

Journal: Journal of Separation Science

An ultra high performance liquid chromatography with photodiode array detection method is developed for the simultaneous quantitative determination of five water‐soluble compounds including danshensu, protocatechualdehyde, rosmarinic acid, salvianolic acid B, and salvianolic acid A in Salvia miltiorrhiza Bge. samples. Through method optimization, the five compounds all expressed good linearity (R2 > 0.9990) in a wide concentration range together...

Read More thumbnail image: Quality evaluation of Salvia miltiorrhiza Bge_ by ultra high performance liquid chromatography with photodiode array detection and chemical fingerprinting coupled with chemometric analysis
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