EarlyView Articles
The very latest articles from the scientific literature
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In‐Depth Proteome Analysis of Ricinus communis Pollens
Published: Dec 14, 2018
Author(s): Fabiano M. Teixeira, Mohibullah Shah, Gilberto B. Domont, Fábio C.S. Nogueira, Francisco A.P. Campos
Journal: PROTEOMICS
Abstract Pollen grains are tiny structures vital for sexual reproduction and consequently seed and fruit production in angiosperms, and a source of many allergenic components responsible for deleterious implications for health worldwide. Current pollen research is mainly focused on unraveling the molecular mechanisms underlying the pollen germination and tube formation passing from the quiescent stage. In this context, an in‐depth...
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A new derivatization method for the determination of valproic acid in serum using tetramethylammonium hydroxide as a catalyst
Published: Dec 14, 2018
Author(s): Guifu Mao, Kun Zhao, Shusen Sun, Yanxia Lu, Xingang Li
Journal: Biomedical Chromatography
Abstract Valproic acid (VPA) pharmacokinetics is highly variable and monitoring of blood levels is necessary to determine its appropriate dosage. This study aimed to establish and validate a novel derivatization method for the determination of VPA. The method was based on the catalytic effect of tetramethylammonium hydroxide using 2,4′‐dibromoacetophenone as a derivatization reagent. After derivatization, samples were injected into...
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Collagen proton fraction from ultrashort echo time magnetization transfer (UTE‐MT) MRI modelling correlates significantly with cortical bone porosity measured with micro‐computed tomography (μCT)
Published: Dec 14, 2018
Author(s): Saeed Jerban, Yajun Ma, Lidi Wan, Adam C. Searleman, Hyungseok Jang, Robert L. Sah, Eric Y. Chang, Jiang Du
Journal: NMR in Biomedicine
Intracortical bone porosity is a key microstructural parameter that determines bone mechanical properties. While clinical MRI visualizes the cortical bone with a signal void, ultrashort echo time (UTE) MRI can acquire high signal from cortical bone, thus enabling quantitative assessments. Magnetization transfer (MT) imaging combined with UTE‐MRI can indirectly assess protons in the bone collagenous matrix, which are inversely related to...
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Retrospective analysis for valproate screening targets with liquid chromatography–high resolution mass spectrometry with positive electrospray ionization: An omics‐based approach
Published: Dec 13, 2018
Author(s): Christian Brinch Mollerup, Brian Schou Rasmussen, Sys Stybe Johansen, Marie Mardal, Kristian Linnet, Petur Weihe Dalsgaard
Journal: Drug Testing and Analysis
Abstract Liquid chromatography coupled with high‐resolution mass spectrometry (LC–HRMS) is an important analytical tool in the systematic toxicological analysis performed in forensic toxicology. However, some important compounds, such as the antiepileptic drug valproate (valproic acid; VPA), cannot be directly detected with positive electrospray ionization (ESI+) due to poor ionization. Here we demonstrate an omics‐based retrospective analysis...
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Quantification of cocaine and cocaine metabolites in dried blood spots from a controlled administration study using liquid chromatography–tandem mass spectrometry
Published: Dec 13, 2018
Author(s): Lars Ambach, Eleanor Menzies, Mark C. Parkin, Andrew Kicman, John R.H. Archer, David M. Wood, Paul I. Dargan, Christophe Stove
Journal: Drug Testing and Analysis
Abstract Cocaine is a common illicit stimulant and is mainly metabolized by hydrolysis to benzoylecgonine (BE) and ecgonine methyl ester (EME), but also to minor metabolites like norcocaine, or hydroxy‐BE. When ethanol is present, cocaethylene is formed. Dried blood spot (DBS) sampling is a minimally invasive microsampling technique with possible advantages for analyte stability and ease of storage, making it an attractive matrix in...
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Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His‐tagged protein
Published: Dec 13, 2018
Author(s): Mengmeng Chang, Qian Qin, Bohong Wang, Tian Xia, Wangjie lv, Xiaoshan Sun, Xianzhe Shi, Guowang Xu
Journal: Journal of Separation Science
Abstract Employing immobilized metal‐ion affinity chromatography and magnetic separation could ideally provide a useful analytical strategy for purifying His‐tagged protein. In the current study, a facile route was designed to prepare CMPEI‐Ni2+@SiO2@Fe3O4 (CMPEI=carboxymethylated polyethyleneimine) magnetic nanoparticles composed of a strong magnetic core of Fe3O4 and a Ni2+‐immobilized carboxymethylated polyethyleneimine coated outside shell,...
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Electric field assisted multicomponent reaction in a microfluidic reactor for superior conversion and yield
Published: Dec 13, 2018
Author(s): Surjendu Maity, Joydip Chaudhuri, Shirsendu Mitra, Saptak Rarotra, Dipankar Bandyopadhyay
Journal: ELECTROPHORESIS
Abstract We explore the improvements in yield and conversion of a chemical reaction inside a two‐phase microfluidic reactor when subjected to an externally applied alternating current (AC) electric field. A computational fluid dynamic (CFD) framework has been developed to incorporate the descriptions of the two‐phase flow, multicomponent transport and reaction, and the Maxwell's stresses generated at oil‐water interface owing to the...
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Separation and simultaneous determination of seven bioactive components in Tripterygium wilfordii Hook. F. and Tripterygium preparations by micellar electrokinetic capillary chromatography
Published: Dec 13, 2018
Author(s): Haitao Guo, Zhiying Wang, Liying Xu, Hongfen Zhang, Ruimiao Chang, Anjia Chen
Journal: ELECTROPHORESIS
Abstract A simple, comprehensive, and highly selective MEKC method has been developed for simultaneous analysis of seven bioactive components (triptolide, wilfortrine, wilfordine, wilforgine, wilforine, triptophenolide, and triptonide) in the root extracts of Tripterygium wilfordii Hook. F. (TWHF) and Tripterygium preparations (TPs). Optimal BGE consisted of 10 mM sodium tetraborate, 30 mM SDS, and 30% v/v methanol. The separation...
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Activation of the hippocampal AC‐cAMP‐PKA‐CREB‐BDNF signaling pathway using WTKYR in depression model rats
Published: Dec 13, 2018
Author(s): Yangzhi Peng, Chengchen Zhang, Yue Su, Zewen Wang, Yong Jiang
Journal: ELECTROPHORESIS
Abstract Depression, also called “depression disorder,” is characterized by a significant and persistent low mood. It has become a major refractory disease in the 21st century. In recent years, Chinese medicine has shown some important clinical value in the treatment of depression. Among them, the Warming and “Tonifying” Kidney‐Yang Recipe (WTKYR) has been demonstrated to have obvious effects in the clinical treatments of...
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Surrogate analyte‐based quantification of main endocannabinoids in whole blood using liquid chromatography–tandem mass spectrometry
Published: Dec 13, 2018
Author(s): Xiaoru Dong, Liliang Li, Yonghong Ye, Dingang Zhang, Lixing Zheng, Yan Jiang, Min Shen
Journal: Biomedical Chromatography
Abstract Endocannabinoids (eCBs) are endogenous ligands of the endocannabinoid system that are known to regulate several physiological and behavioral processes. Previous studies have developed methods for the detection of main eCBs including arachidonylethanolamide (AEA) and 2‐arachidonoylglycerol (2‐AG), mostly in serum or plasma. Whole blood is a superior biomaterial for eCBs analysis owing to the nature of the shortened isolation...
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A liquid chromatography–tandem mass spectrometry method for preclinical pharmacokinetics and tissue distribution of hydrolyzable tannins chebulinic acid and chebulagic acid in rats
Published: Dec 13, 2018
Author(s): Ying Lu, Huiyu Yan, Shiyong Teng, Xige Yang
Journal: Biomedical Chromatography
Abstract A simple and sensitive liquid chromatography–tandem mass spectrometry method was developed for the simultaneous determination of chebulinic acid and chebulagic acid in rat plasma and tissues and well used in the pharmacokinetic and tissue distribution studies after intraperitoneal injection administration. Samples were processed with methanol by protein precipitation, and chromatographic separation was performed on an...
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Simultaneous detection of multiple hydroxylated polychlorinated biphenyls from biological samples using ultra‐high‐performance liquid chromatography with isotope dilution tandem mass spectrometry
Published: Dec 13, 2018
Author(s): Yibin Zheng, Delei Cai, Baifen Huang, Jianlong Han, Qing Chen, Jingshun Zhang, Jie Zhang, Xiaofeng Wang, Haitao Shen
Journal: Journal of Separation Science
Abstract We established a method for the separation and detection of nine hydroxylated polychlorinated biphenyls in whole blood and urine samples using ultra high performance liquid chromatography coupled with electrospray negative ionization tandem mass spectrometry. Clean‐up procedures involved a filtration step, and optimization involved a pretreatment step consisting of a simple liquid–liquid extraction using hydrated silica‐gel...
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XPS study of silicon spheres as a density standard
Published: Dec 13, 2018
Author(s): Lulu Zhang, Naoki Kuramoto, Akira Kurokawa, Kenichi Fujii
Journal: Surface and Interface Analysis
As density is one of the basic physical properties of materials, an accurate density standard is absolutely essential. To determine density with high accuracy and precision, two 1‐kg single‐crystal silicon spheres (NMIJ‐S4 and NMIJ‐S5) are used as the primary standard at the National Metrology Institute of Japan. For the accurate measurement of the mass and volume of the silicon spheres, the effects of the surface layer must be carefully...
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A smartphone‐based on‐site nucleic acid testing platform at point‐of‐care settings
Published: Dec 13, 2018
Author(s): Xiayu Xu, Xuemin Wang, Jie Hu, Yang Gong, Lin Wang, Wan Zhou, XiuJun Li, Feng Xu
Journal: ELECTROPHORESIS
Abstract We developed a smartphone‐based on‐site nucleic acid testing (NAT) platform that can image and analyze lateral flow nucleic acid assays at point‐of‐care settings. An inexpensive add‐on was devised to run lateral flow assays while providing homogeneous ambient light for imaging. In addition, an Android app with a user‐friendly interface was developed for the result analysis and management. Linear color calibration is...
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Numerical investigation of the current transition regimes in nanochannels
Published: Dec 13, 2018
Author(s): Arturo Villegas, David Berardi, Francisco Javier Diez
Journal: ELECTROPHORESIS
Abstract The concentration polarization phenomena and its effects represent one of the main challenges for the optimal operation of many nanofluidic systems. A numerical investigation of the different electric current transition regimes observed during the concentration polarization phenomena in nanochannels is performed. This included a 2D‐axisymmetric simulation of the nanofluidic system (reservoir‐nanochannel‐reservoir). From...
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Nanopore sequencing: An enrichment‐free alternative to mitochondrial DNA sequencing
Published: Dec 13, 2018
Author(s): Roxanne R. Zascavage, Kelcie Thorson, John V. Planz
Journal: ELECTROPHORESIS
Abstract Mitochondrial DNA sequence data are often utilized in disease studies, conservation genetics and forensic identification. The current approaches for sequencing the full mtGenome typically require several rounds of PCR enrichment during Sanger or MPS protocols followed by fairly tedious assembly and analysis. Here we describe an efficient approach to sequencing directly from genomic DNA samples without prior enrichment or...
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High‐throughput 96‐well solid‐phase extraction for preparation of tetracycline followed by liquid chromatography and mass spectrometry analysis
Published: Dec 13, 2018
Author(s): Kang Chen, Jie Wang, Shiyan Li, Yang Wang, Yiqi Zhang, Qing Shen
Journal: ELECTROPHORESIS
Abstract Tetracyclines abuse has frequently occurred in aquaculture against bacteria, rickettsiae, spirochetes, and mycoplasmas. In this study, a high‐throughput sample preparation method was developed using 96‐well plate solid‐phase extraction (p‐SPE) and the extract was analyzed by ultra‐performance liquid chromatography tandem mass spectrometry (UPLC‐MS/MS). The experimental conditions were optimized such that the pH is 4, the eluting...
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Assessment of submicron particle zeta potential in simple electrokinetic microdevices
Published: Dec 13, 2018
Author(s): Samuel Hidalgo‐Caballero, Cody Justice Lentz, Blanca H. Lapizco‐Encinas
Journal: ELECTROPHORESIS
Abstract The present communication illustrates the use of simple electrokinetic devices for the assessment of the zeta potential of submicron polystyrene particles. A combination of manual and automatic particle tracking was employed. This approach allows for characterizing particles in the same conditions and devices in which they can be separated, e.g. dielectrophoretic separations; making the resulting data readily applicable. ...
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Raman spectroscopy analysis of new copper‐cysteamine photosensitizer
Published: Dec 13, 2018
Author(s): Hussein Akafzade, Suresh C. Sharma, Nader Hozhabri, Wei Chen, Lun Ma
Journal: Journal of Raman Spectroscopy
Abstract Raman spectroscopy and several microstructure analysis techniques have been used to better characterize recently synthesized copper‐cysteamine Cu3Cl(SR)2, where R = CH2CH2NH2. Nanoparticles of this new copper‐cysteamine have been identified as having potential applications in radiation detection and cancer treatment because of the fact that they can be activated by light, X‐rays, ultrasound, and microwave radiation ...
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Infused self‐assembly on Langmuir–Blodgett Film: Fabrication of highly efficient SERS active substrates with controlled plasmonic aggregates
Published: Dec 13, 2018
Author(s): Somsubhra Saha, Manash Ghosh, Joydeep Chowdhury
Journal: Journal of Raman Spectroscopy
Abstract A facile procedure towards the fabrication of highly reproducible, large area surface‐enhanced Raman scattering (SERS) active substrates through integration of Langmuir–Blodgett and self‐assembly technique has been reported. The plasmonic architectures of the substrates can be tuned at will to control the hot spots and hence the overall enhancements of SERS signal. The as‐prepared substrates of classes “A” and “B” incubated...
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