EarlyView Articles
The very latest articles from the scientific literature
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Dual‐frequency irradiation CEST‐MRI of endogenous bulk mobile proteins
Published: Apr 19, 2018
Author(s): Steffen Goerke, Johannes Breitling, Moritz Zaiss, Johannes Windschuh, Patrick Kunz, Patrick Schuenke, Daniel Paech, Dario L. Longo, Karel D. Klika, Mark E. Ladd, Peter Bachert
Journal: NMR in Biomedicine
A novel MRI contrast is proposed which enables the selective detection of endogenous bulk mobile proteins in vivo. Such a non‐invasive imaging technique may be of particular interest for many diseases associated with pathological alterations of protein expression, such as cancer and neurodegenerative disorders. Specificity to mobile proteins was achieved by the selective measurement of intramolecular spin diffusion and the removal of semi‐solid...
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NMR‐based metabolomics of biofluids in cancer
Published: Apr 19, 2018
Author(s): Guro F. Giskeødegård, Torfinn S. Madssen, Leslie R. Euceda, May‐Britt Tessem, Siver A. Moestue, Tone F. Bathen
Journal: NMR in Biomedicine
Metabolomics is the branch of “omics” technologies that involves high‐throughput identification and quantification of small‐molecule metabolites in the metabolome. NMR‐based spectroscopy of biofluids represents a potential method for non‐invasive characterization of cancer. While the metabolism of cancer cells is altered compared with normal non‐proliferating cells, the metabolome of several biofluids (e.g. blood and urine) reflects the...
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Liquid chromatography–tandem MS/MS method for simultaneous quantification of paracetamol, chlorzoxazone and aceclofenac in human plasma: An application to a clinical pharmacokinetic study
Published: Apr 18, 2018
Author(s): Dalia Mohamed, Maha A. Hegazy, Mona S. Elshahed, Safaa S. Toubar, Marwa I. Helmy
Journal: Biomedical Chromatography
Abstract A facile, fast and specific method based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) for the simultaneous quantitation of paracetamol, chlorzoxazone and aceclofenac in human plasma was developed and validated. Sample preparation was achieved by liquid–liquid extraction. The analysis was performed on a reversed‐phase C18 HPLC column (5 μm, 4.6 × 50 mm) using acetonitrile–10 mM ammonium formate pH 3.0 (65:35, v/v) as the...
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Quality by design‐assisted development of a capillary electrophoresis method for the chiral purity determination of dexmedetomidine
Published: Apr 18, 2018
Author(s): Sulaiman Krait, Gerhard K. E. Scriba
Journal: ELECTROPHORESIS
Abstract Dexmedetomidine is a selective α2‐adrenergic agonist used for patient sedation, while its enantiomer levomedetomidine has no sedative effects. As CE has been shown to be a powerful technique for enantiomer analysis, the aim of the study was the quality by design‐based development of a CE‐based limit test for the enantiomeric impurity levomedetomidine. The analytical target profile was defined that the method should be able...
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Simultaneous determination of twelve polar pteridines including dihydro‐ and tetrahydropteridine in human urine by hydrophilic interaction liquid chromatography with tandem mass spectrometry
Published: Apr 18, 2018
Author(s): Xin Xiong, Yuanyuan Zhang, Wenjing Zhang
Journal: Biomedical Chromatography
Abstract Pteridines and their derivatives are important cofactors in the process of cell metabolism, and the level of urinary excretion of these compounds is considered as an important clinical criterion. In this work, a new separation method involving hydrophilic interaction chromatography (HILIC) with tandem mass spectrometric detection has been developed for the simultaneous analysis of 12 pteridines including oxidized, di‐ and...
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Validated LC–MS/MS method for the quantification of sciadopitysin in rat plasma and its application to pharmacokinetic and bioavailability studies in vivo
Published: Apr 18, 2018
Author(s): Shenbao Yang, Ruiying Qu, Zhe Zhu, Wei Li, Chengliang Zhao, Liantai Li
Journal: Biomedical Chromatography
Abstract A sensitive and rapid LC–MS/MS method was developed and validated for quantitation of sciadopitysin in rat plasma using amentoflavone as an internal standard. Sample processing was accomplished after deproteinization with 150 μL aliquot of acetonitrile. Chromatographic separation was achieved using an Agela C18 column with an isocratic mobile phase comprising 2 mm ammonium acetate–acetonitrile (35:65, v/v) at a flow rate of 0.4 mL/min. Detection...
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Use of capillary electrophoresis for characterisation of vinyl‐terminated Au nanoprisms and nanooctahedra
Published: Apr 18, 2018
Author(s): Carlos Adelantado, Manuel Algarra, Mohammed Zougagh, Ángel Ríos
Journal: ELECTROPHORESIS
Abstract It is described a simple, rapid and efficient methodology to characterise and separate gold nanoprisms and nanooctahedra by capillary electrophoresis. This technique is suitable to distinguish between morphologies and it can be used as a powerful separation tool after a customised synthesis of both structures. This synthesis was carried out by amending two parameters, temperature and pH, and a sharp decrease was found in...
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Determination of phenolic compounds in ancient and modern durum wheat genotypes
Published: Apr 18, 2018
Author(s): Alessandro Di Loreto, Sara Bosi, Lidia Montero, Valeria Bregola, Ilaria Marotti, Rocco Enrico Sferrazza, Giovanni Dinelli, Miguel Herrero, Alejandro Cifuentes
Journal: ELECTROPHORESIS
Abstract Due to its significant amount of antioxidants, durum wheat (Triticum turgidum ssp. durum) could potentially contribute to the protection against a number of chronic diseases, such as diabetes, cardiovascular disease, and cancer. The increasing interest toward healthy food among both consumers and scientists has moved the focus toward the phytochemical content of whole wheat grains. The aim of this study was to identify the...
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T‐Cell Immunopeptidomes Reveal Cell Subtype Surface Markers Derived From Intracellular Proteins
Published: Apr 18, 2018
Author(s): Niclas Olsson, Liora M. Schultz, Lichao Zhang, Michael S. Khodadoust, Rupa Narayan, Debra K. Czerwinski, Ronald Levy, Joshua E. Elias
Journal: PROTEOMICS
Abstract Immunopeptidomes promise novel surface markers as ideal immunotherapy targets, but their characterization by mass spectrometry (MS) remains challenging. Until recently, cell numbers exceeding 109 were needed to survey thousands of HLA ligands. Such limited analytical sensitivity has historically constrained the types of clinical specimens that can be evaluated to cell cultures or bulk tissues. Measuring immunopeptidomes from purified...
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Quantitative assessment of mAb Fc glycosylation of CQA importance by capillary electrophoresis
Published: Apr 18, 2018
Author(s): Marton Szigeti, Jeff Chapman, Beata Borza, Andras Guttman
Journal: ELECTROPHORESIS
Abstract The attached carbohydrates at the highly conserved asparagine‐linked glycosylation site in the CH2 domain of the fragment crystallizable (Fc) region of monoclonal antibody therapeutics can play an essential role in their mechanism of action, including ADCC, CDC, anti‐inflammatory functions, and serum half‐life. Thus, this particular glycosylation represents one of the important critical quality attributes (CQA) of therapeutic...
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Comparative Protein Interaction Network Analysis Identifies Shared and Distinct Functions for the Human ROCO Proteins
Published: Apr 17, 2018
Author(s): James E. Tomkins, Sybille Dihanich, Alexandra Beilina, Raffaele Ferrari, Nicolò Ilacqua, Mark R. Cookson, Patrick A. Lewis, Claudia Manzoni
Journal: PROTEOMICS
Abstract Signal transduction cascades governed by kinases and GTPases are a critical component of the command and control of cellular processes, with the precise outcome partly determined by direct protein–protein interactions (PPIs). Here, we use the human ROCO proteins as a model for investigating PPI signaling events—taking advantage of the unique dual kinase/GTPase activities and scaffolding properties of these multidomain...
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Electrodeposition of poly(acrylic acid) on stainless steel with enhanced adhesion properties
Published: Apr 17, 2018
Author(s): D. Meinderink, A. G. Orive, G. Grundmeier
Journal: Surface and Interface Analysis
A facile electrochemical methodology to prepare poly(acrylic acid) (PAA) thin films in mildly acid aqueous solutions onto stainless steel is presented. Together with the PAA, the codeposition of Zn species is observed. The latter can be further removed by rinsing with dilute HCl aqueous solutions. These PAA‐modified steel samples have been characterized by infrared reflection absorption spectroscopy and atomic force microscopy and exhibit...
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Comment on I ntensity modulation of the Shirley background of the C r3p spectra with photon energies around the C r2p edge by A H errera G omez D C abrera G erman A D D utol et al S urface I nterface A nal 2018 50 246252
Published: Apr 17, 2018
Author(s): R. Bavand, L. Chen, R. França, S. Loran, D.‐Q. Yang, A. Yelon, G.‐X. Zhang, E. Sacher
Journal: Surface and Interface Analysis
We show that, while the Shirley background lacks a theoretical basis, its use satisfies the condition for the proper modeling of all these backgrounds when properly applied. We present these four conditions of applicability, discuss their use, and indicate where their lack of use gives misleading results.
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Study of the Au‐Cr bilayer system using X‐ray reflectivity, GDOES, and ToF‐SIMS
Published: Apr 17, 2018
Author(s): Philippe Jonnard, Mohammed H. Modi, Karine Le Guen, Nargish Aneshwari, Mangalika Sinha, Mourad Idir, Patrick Chapon, Anouk Galtayries
Journal: Surface and Interface Analysis
We study a Au (25 nm)/Cr (10 nm) bilayer system as a model of mirror for the soft X‐ray energy range. The Au and Cr thin films are a few nanometer thick and are deposited on a float glass substrate. The sample is characterized by using 3 complementary techniques: soft X‐ray reflectivity, glow discharge optical emission spectrometry (GDOES), and time‐of‐flight secondary ion mass spectroscopy (ToF‐SIMS). Soft X‐ray reflectivity...
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Investigation of work function and chemical composition of thin films of borides and nitrides
Published: Apr 17, 2018
Author(s): A. Mezzi, P. Soltani, S. Kaciulis, A. Bellucci, M. Girolami, M. Mastellone, D. M. Trucchi
Journal: Surface and Interface Analysis
Thin films of various borides, nitrides, and barium fluorides were tentatively deposited by pulsed laser deposition and by magnetron sputtering in order to develop the components of thermionic‐photovoltaic devices for the high‐temperature thermal to electrical conversion by solid state. To improve the device performance, the materials characterized by a low work function were selected. In the present work, the chemical composition and...
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Depth analysis of Al/ZrC interfaces using SIMS and x‐ray reflectivity
Published: Apr 17, 2018
Author(s): Mohammed H. Modi, Mangalika Sinha, Aniruddha Bose, Amol Singh, Philippe Jonnard
Journal: Surface and Interface Analysis
Al/ZrC/Al/W multilayer structure is suitable for waveguide applications in the hard X‐ray region in order to confine the wave field in a nanometer‐thick layer. Intermixing of Al at the interfaces is a serious problem to achieve the expected performances from an experimentally grown multilayer. In the present study, the effect of a carbon capping layer in a C/Al/ZrC/Al/W waveguide structure is determined using time of flight secondary ion...
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Improved interfacial properties of PI composites through graphene oxide and carbon nanotubes on carbon fiber surface
Published: Apr 17, 2018
Author(s): Lv Yongqiang, Peng Chunzheng
Journal: Surface and Interface Analysis
Carbon nanotubes (CNTs) have been identified as excellent nanoreinforcements for carbon fiber (CF)–reinforced polymers regarding a wide range of engineering applications. The outstanding properties of CNTs, such as their large surface area, high mechanical strength, and low manufacturing cost bring them to be distinguished nanoreinforcements for carbon fiber–reinforced polymers to form multifunctional and multiscale composites....
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Surface and redox characterization of new nanostructured ZrO 2 @CeO 2 systems with potential catalytic applications
Published: Apr 17, 2018
Author(s): Adrián Barroso‐Bogeat, Bernardo Núñez‐Pérez, Ginesa Blanco, José María Pintado, Juan Carlos Hernández‐Garrido, José Juan Calvino
Journal: Surface and Interface Analysis
Ceria‐zirconia mixed oxides are widely used as catalysts and catalytic supports. Nevertheless, due to economic and geo‐strategic concerns, reducing and optimizing the lanthanide content in these formulations while preserving or even improving their excellent redox and catalytic properties has become a challenge for current research. In this context, the design of core@shell nanostructures arises as a feasible alternative to achieve the...
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Cascade Mi (i = 1–5) subshell X ‐ray emission at incident photon energies across the Lj (j = 1–3) subshell absorption edges of 66Dy
Published: Apr 17, 2018
Author(s): Rajnish Kaur, Anil Kumar, Mateusz Czyzycki, Alessandro Migliori, Andreas G. Karydas, Sanjiv Puri
Journal: X-Ray Spectrometry
The total M shell and the Mk (k = ξ, αβ, γ, m) X‐ray production cross sections for 66Dy have been measured at incident photon energies across its Lj (j = 1–3) subshell absorption edge energies, ranging 7.8–9.2 keV. This study aims to investigate the evolution of the probability for cascade decay of Lj subshell vacancies as the tunable incident energy ionizes progressively different 66Dy Lj subshells. The experimental X‐ray production...
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Raman spectroscopy of the photosensitive pigment Prussian blue
Published: Apr 17, 2018
Author(s): Giulia Moretti, Claire Gervais
Journal: Journal of Raman Spectroscopy
Abstract Prussian blue is a pigment highly sensitive to electromagnetic radiation, visible light included. This photosensitivity, associated with a complex redox behavior, causes a vulnerability even to Raman lasers, with the possibility of sample alteration or irreversible damage. In this study, we systematically explored the influence of the laser wavelength and laser power on different types of Prussian blue pigments, soluble and...
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