EarlyView Articles
The very latest articles from the scientific literature
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Chromatographic analysis of tryptophan metabolites
Published: Jun 26, 2017
Author(s): Ilona Sadok, Andrzej Gamian, Magdalena Maria Staniszewska
Journal: Journal of Separation Science
The kynurenine pathway generates multiple tryptophan metabolites called collectively kynurenines and leads to formation of the enzyme cofactor nicotinamide adenine dinucleotide. The first step in this pathway is tryptophan degradation, initiated by the rate‐limiting enzymes indoleamine 2,3‐dioxygenase, or tryptophan 2,3‐dioxygenase, depending on the tissue. The balanced kynurenine metabolism, which has been a subject of multiple studies...
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Dispersive liquid–liquid microextraction of phenolic compounds from vegetable oils using a magnetic ionic liquid
Published: Jun 26, 2017
Author(s): Shuqiang Zhu, Lijun Wang, Along Su, Haixia Zhang
Journal: Journal of Separation Science
A novel method was developed for the determination of two endocrine‐disrupting chemicals, bisphenol A and 4‐nonylphenol, in vegetable oil by dispersive liquid–liquid microextraction followed by ultra high performance liquid chromatography with tandem mass spectrometry. Using a magnetic liquid as the microextraction solvent, several key parameters were optimized, including the type and volume of the magnetic liquid, extraction time, amount...
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Proteomic profiling of the hypothalamus in two mouse models of narcolepsy
Published: Jun 26, 2017
Author(s): Sausan Azzam, Daniela Schlatzer, David Nethery, Dania Saleh, Xiaolin Li, Afaf Akladious, Mark R. Chance, Kingman P. Strohl
Journal: PROTEOMICS
Narcolepsy is a disabling neurological disorder of sleepiness linked to the loss of neurons producing orexin neuropeptides in the hypothalamus. Two well‐characterized phenotypic mouse models of narcolepsy, loss‐of‐function (orexin‐knockout), and progressive loss of orexin (orexin/ataxin‐3) exist. The open question is whether the proteomics signatures of the hypothalamus would be different between the two models. To address this gap, we...
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Separation of single‐base sequential isomers of single‐stranded DNA by capillary electrophoresis and its application in the discrimination of single‐base DNA mutations
Published: Jun 26, 2017
Author(s): Takao Sakurai, Hitoshi Hoshino, Toru Takahashi
Journal: Journal of Separation Science
Separation of single‐base substitution sequential DNA isomers remains one of the most challenging tasks in DNA separation by capillary electrophoresis. We developed a simple, versatile capillary electrophoresis technique for the separation of single‐base sequential isomers of DNA having the same chain length. This technique is based on charge differences resulting from the different protonation (acid dissociation) properties of the four...
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Application of capability indices and control charts in the analytical method control strategy
Published: Jun 26, 2017
Author(s): Alexis Oliva, Matías Llabres Martinez
Journal: Journal of Separation Science
In this study, we assessed the usefulness of control charts in combination with the process capability indices, Cpm and Cpk, in the control strategy of an analytical method. The traditional X‐chart and moving range chart were used to monitor the analytical method over a 2‐year period. The results confirmed that the analytical method is in‐control and stable. Different criteria were used to establish the specifications limits (i.e. analyst...
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Efficient approach for the extraction of proanthocyanidins from Cinnamomum longepaniculatum leaves using ultrasonic irradiation and an evaluation of their inhibition activity on digestive enzymes and antioxidant activity in vitro
Published: Jun 23, 2017
Author(s): Zaizhi Liu, Kailin Mo, Shimin Fei, Yuangang Zu, Lei Yang
Journal: Journal of Separation Science
Proanthocyanidins were separated for the first time from Cinnamomum longepaniculatum leaves. An experiment‐based extraction strategy was used to research the efficiency of an ultrasound‐assisted method for proanthocyanidins extraction. The Plackett–Burman design results revealed that the ultrasonication time, ultrasonic power and liquid/solid ratio were the most significant parameters among the six variables in the extraction process. Upon further ...
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Trypsin inhibitor screening in traditional Chinese medicine by using an immobilized enzyme microreactor in capillary and molecular docking study
Published: Jun 23, 2017
Author(s): Mengxia Cheng, Zilin Chen
Journal: Journal of Separation Science
A trypsin immobilized enzyme microreactor was successfully prepared in capillary for studying enzyme kinetics of trypsin and online screening of trypsin inhibitors from traditional Chinese medicine through capillary electrophoresis. Trypsin was immobilized on the inner wall at the inlet of the capillary treated with polydopamine. The rest of the capillary was used as a separation channel. The parameters including the separation efficiency...
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Development of a novel hollow‐fiber liquid‐phase microextraction based on oil‐in‐salt and its comparison with conventional one
Published: Jun 23, 2017
Author(s): Miao‐miao Li, Shuang Hu, Xuan Chen, Xiao‐hong Bai
Journal: Journal of Separation Science
A novel hollow‐fiber liquid‐phase microextraction based on oil‐in‐salt was proposed and introduced for the simultaneous extraction and enrichment of the main active compounds of hesperidin, honokiol, shikonin, magnolol, emodin, and β,β′‐dimethylacrylshikonin in a formula of Zi‐Cao‐Cheng‐Qi decoction and the single herb, Fructus Aurantii Immaturus, Cortex Magnoliae Officinalis, Radix et Rhizoma, and Lithospermum erythrorhizon, composing the formula...
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Direct comparison of high‐temporal‐resolution CINE MRI with Doppler ultrasound for assessment of diastolic dysfunction in mice
Published: Jun 23, 2017
Author(s): Thomas A. Roberts, Anthony N. Price, Laurence H. Jackson, Valerie Taylor, Anna L. David, Mark F. Lythgoe, Daniel J. Stuckey
Journal: NMR in Biomedicine
Diastolic dysfunction is a sensitive early indicator of heart failure and can provide additional data to conventional measures of systolic function. Transmitral Doppler ultrasound, which measures the one‐dimensional flow of blood through the mitral valve, is currently the preferred method for the measurement of diastolic function, but the measurement of the left ventricular volume changes using high‐temporal‐resolution cinematic magnetic...
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Uncorrelated component analysis on manifold for statistical process monitoring
Published: Jun 23, 2017
Author(s): Qilong Jia, Yingwei Zhang, Lirong Zhai, Lin Feng
Journal: Journal of Chemometrics
A novel process modeling approach referred to as locally invariant uncorrelated component analysis (LIUCA) is proposed for the purpose of statistical process monitoring. The contributions are as follows: (1) LIUCA intends to find a part‐based representation subspace in which two data points are close to each other, if they are close in the k‐nearest neighbor graph; (2) LIUCA can exploit the geometrical structure of the data space, which will improve...
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Quantitative proteomic analysis of host epithelial cells infected by Salmonella enterica serovar Typhimurium
Published: Jun 23, 2017
Author(s): Linlu Qi, Mo Hu, Jiaqi Fu, Yanhua Liu, Mei Wu, Kaiwen Yu, Xiaoyun Liu
Journal: PROTEOMICS
Systems‐level analyses have the capability to offer new insight into host–pathogen interactions on the molecular level. Using Salmonella infection of host epithelial cells as a model system, we previously analyzed intracellular bacterial proteome as a window into pathogens’ adaptations to their host environment [Infect. Immun. 2015; J. Proteome Res. 2017]. Herein we extended our efforts to quantitatively examine protein expression of host cells...
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Proteomic analysis of hair shafts from monozygotic twins: Expression profiles and genetically variant peptides
Published: Jun 23, 2017
Author(s): Pei‐Wen Wu, Katelyn E. Mason, Blythe P. Durbin‐Johnson, Michelle Salemi, Brett S. Phinney, David M. Rocke, Glendon J. Parker, Robert H. Rice
Journal: PROTEOMICS
Forensic association of hair shaft evidence with individuals is currently assessed by comparing mitochondrial DNA haplotypes of reference and casework samples, primarily for exclusionary purposes. Present work tests and validates more recent proteomic approaches to extract quantitative transcriptional and genetic information from hair samples of monozygotic twin pairs, which would be predicted to partition away from unrelated individuals...
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Ephedra alkaloid contents of Chinese herbal formulae sold in Taiwan
Published: Jun 23, 2017
Author(s): Chih‐Wei Chang, Szu‐Yun Hsu, Guan‐Qian Huang, Mei‐Chich Hsu
Journal: Drug Testing and Analysis
Consumption of Ephedra alkaloids is prohibited in‐competition by the World Anti‐Doping Agency (WADA). In Taiwan, colds are often treated with Chinese herbal formulae containing Herba Ephedrae. We screened products sold in Taiwan and preliminarily assessed their relationships with WADA threshold violations. Fifty‐six concentrated powder products, including 19 Chinese herbal formulae that contained Herba Ephedrae, were collected. The...
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Diffusion MRI microstructure models with in vivo human brain Connectome data: results from a multi‐group comparison
Published: Jun 23, 2017
Author(s): Uran Ferizi, Benoit Scherrer, Torben Schneider, Mohammad Alipoor, Odin Eufracio, Rutger H.J. Fick, Rachid Deriche, Markus Nilsson, Ana K. Loya‐Olivas, Mariano Rivera, Dirk H.J. Poot, Alonso Ramirez‐Manzanares, Jose L. Marroquin, Ariel Rokem, Christian Pötter, Robert F. Dougherty, Ken Sakaie, Claudia Wheeler‐Kingshott, Simon K. Warfield, Thomas Witzel, Lawrence L. Wald, José G. Raya, Daniel C. Alexander
Journal: NMR in Biomedicine
A large number of mathematical models have been proposed to describe the measured signal in diffusion‐weighted (DW) magnetic resonance imaging (MRI). However, model comparison to date focuses only on specific subclasses, e.g. compartment models or signal models, and little or no information is available in the literature on how performance varies among the different types of models. To address this deficiency, we organized the ‘White...
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Influence of bleaching and coloring on ethyl glucuronide content in human hair
Published: Jun 22, 2017
Author(s): Silvana Petzel‐Witt, Werner Pogoda, Cora Wunder, Alexander Paulke, Manfred Schubert‐Zsilavecz, Stefan W. Toennes
Journal: Drug Testing and Analysis
Ethyl glucuronide (EtG) is increasingly used in forensic toxicology as a marker for alcohol use in analyses of hair samples, especially in abstinence control. Some cosmetic treatments are considered to markedly reduce the EtG content. In view of especially many women with coloured hair the present study was performed to further investigate the effect of a variety of colouring procedures (bleaching, tinting, permanent and semi‐permanent...
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Syringe‐to‐syringe dispersive liquid‐phase microextraction solidified floating organic drop combined with high‐performance liquid chromatography for the separation and quantification of ochratoxin A in food samples
Published: Jun 22, 2017
Author(s): Mohammad Asadi
Journal: Journal of Separation Science
A new, simple, and rapid syringe‐to‐syringe dispersive liquid‐phase microextraction with solidified floating organic drop was used for the separation and preconcentration of ochratoxin A from grain and juice samples before its quantification using high‐performance liquid chromatography and fluorescence detection. Factors influencing the microextraction efficiency of ochratoxin A, such as sample solution pH, type and volume of organic...
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Modified headspace solid‐phase microextraction for the determination of quantitative relationships between components of mixtures consisting of alcohols, esters, and ethers — impact of the vapor pressure difference of the compounds
Published: Jun 22, 2017
Author(s): Andrzej Lech Dawidowicz, Joanna Szewczyk, Michal P. Dybowski
Journal: Journal of Separation Science
The quantitative relationship between analytes established by the headspace solid‐phase microextraction procedure for multicomponent mixtures depends not only on the character and strength of interactions of individual components with solid‐phase microextraction fiber but also on their vapor pressure in the applied headspace solid‐phase microextraction system. This study proves that vapor pressure is of minor importance when the sample is...
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Comparison between chaotropic and detergent‐based sample preparation workflow in tendon for mass spectrometry analysis
Published: Jun 22, 2017
Author(s): Yalda Ashraf Kharaz, Danae Zamboulis, Karen Sanders, Eithne Comerford, Peter Clegg, Mandy Peffers
Journal: PROTEOMICS
Exploring the tendon proteome is a challenging but important task for understanding the mechanisms of physiological/pathological processes during ageing and disease and for the development of new treatments. Several extraction methods have been utilised for tendon mass spectrometry, however different extraction methods have not been simultaneously compared. In the present study we compared protein extraction in tendon with two chaotropic...
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Aptamer‐functionalized Fe 3 O 4 magnetic nanoparticles as a solid‐phase extraction adsorbent for the selective extraction of berberine from Cortex phellodendri
Published: Jun 22, 2017
Author(s): Ling‐Feng Jiang, Bo‐Cheng Chen, Ben Chen, Xue‐Jian Li, Hai‐Lin Liao, Wen‐Yan Zhang, Lin Wu
Journal: Journal of Separation Science
The extraction adsorbent was fabricated by immobilizing the highly specific recognition and binding of aptamer onto the surface of Fe3O4 magnetic nanoparticles, which not only acted as recognition elements to recognize and capture the target molecule berberine from the extract of Cortex phellodendri, but also could favor the rapid separation and purification of the bound berberine by using an external magnet. The developed solid‐phase...
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Coaxial flow‐gating interface for capillary electrophoresis
Published: Jun 22, 2017
Author(s): František Opekar, Petr Tůma
Journal: Journal of Separation Science
A coaxial flow‐gating interface is described in which the separation capillary passes through the sampling capillary. Continuous flow of the sample solution flowing out of the sampling capillary is directed away from the injection end of the separation capillary by counter‐current flow of the gating solution. During the injection, the flow of the gating solution is interrupted, so that a plug of solution is formed at the inlet into the...
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