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Morphine and codeine in oral fluid after controlled poppy seed administration

Published: Oct 24, 2014

Author(s): Marta Concheiro, Matthew N. Newmeyer, Jose Luiz Costa, Ron Flegel, David A. Gorelick, Marilyn A. Huestis

Journal: Drug Testing and Analysis

Opiates are an important drug class in drug testing programmes. Ingestion of poppy seeds containing morphine and codeine can yield positive opiate tests and mislead result interpretation in forensic and clinical settings. Multiple publications evaluated urine opiate concentrations following poppy seed ingestion, but only two addressed oral fluid (OF) results; neither provided the ingested morphine and codeine dosage. We administered two...

Read More thumbnail image: Morphine and codeine in oral fluid after controlled poppy seed administration

Significant enhancement of 11‐Hydroxy‐THC detection by formation of picolinic acid esters and application of liquid chromatography/multi stage mass spectrometry (LC‐MS 3 ): Application to hair and oral fluid analysis

Published: Oct 24, 2014

Author(s): Detlef Thieme, Ulf Sachs, Hans Sachs, Christine Moore

Journal: Drug Testing and Analysis

Formation of picolinic acid esters of hydroxylated drugs or their biotransformation products is a promising tool to improve their mass spectrometric ionization efficiency, alter their fragmentation behaviour and enhance sensitivity and specificity of their detection. The procedure was optimized and tested for the detection of cannabinoids, which proved to be most challenging when dealing with alternative specimens, for example hair and...

Read More thumbnail image: Significant enhancement of 11HydroxyTHC detection by formation of picolinic acid esters and application of liquid chromatographymulti stage mass spectrometry LCMS 3  Application to hair and oral fluid analysis

Synthetic cannabimimetic agents metabolized by carboxylesterases

Published: Oct 24, 2014

Author(s): Ragnar Thomsen, Line M. Nielsen, Niels B. Holm, Henrik B. Rasmussen, Kristian Linnet, 

Journal: Drug Testing and Analysis

Synthetic cannabimimetic agents are a large group of diverse compounds which act as agonists at cannabinoid receptors. Since 2004, synthetic cannabinoids have been used recreationally, although several of the compounds have been shown to cause severe toxicity in humans. In this study, the metabolism of two indazole carboxamide derivatives, AB‐PINACA and AB‐FUBINACA, was investigated by using human liver microsomes (HLM). For both...

Read More thumbnail image: Synthetic cannabimimetic agents metabolized by carboxylesterases

Development and validation of a single HPLC method for determination of α ‐tocopherol in cell culture and in human or mouse biological samples

Published: Oct 24, 2014

Author(s): Henar M. Cimadevilla, David Hevia, Ana Miar, Juan C. Mayo, Felipe Lombo, Rosa M. Sainz

Journal: Biomedical Chromatography

ABSTRACT A straightforward and common analytical method for α‐tocopherol (αT) determination in various biological samples, including plasma, red blood cells (RBC), tissues and cultured cell lines, was developed and validated, using a reverse phase‐chromatographic method (RP‐HPLC). Even though many chromatographic methods for αT determination have been reported, most of them require readjustment when applied to different types of samples. Thus,...

Read More thumbnail image: Development and validation of a single HPLC method for determination of  tocopherol in cell culture and in human or mouse biological samples

Methylhexanamine is not detectable in Pelargonium or Geranium species and their essential oils: A multi‐centre investigation

Published: Oct 23, 2014

Author(s): Mahmoud A. ElSohly, Waseem Gul, Candice Tolbert, Kareem M. ElSohly, Timothy P. Murphy, Bharathi Avula, Amar G. Chittiboyina, Mei Wang, Ikhlas A. Khan, Min Yang, Dean Guo, Wei‐Dong Zhang, Juan Su

Journal: Drug Testing and Analysis

In an earlier study, we developed two sensitive and reliable procedures for gas chromatography‐mass spectrometry (GC‐MS) and liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analysis of methylhexaneamine (MHA) in P. graveolens plant materials and volatile oils. None of the analyzed plant materials or oils showed any detectable levels of MHA which was further substantiated by high resolution liquid chromatography‐quantum time of flight‐mass...

Read More thumbnail image: Methylhexanamine is not detectable in Pelargonium or Geranium species and their essential oils A multicentre investigation

Measuring renal tissue relaxation times at 7 T

Published: Oct 23, 2014

Author(s): Xiufeng Li, Patrick J. Bolan, Kamil Ugurbil, Gregory J. Metzger

Journal: NMR in Biomedicine

As developments in RF coils and RF management strategies make performing ultra‐high‐field renal imaging feasible, understanding the relaxation times of the tissue becomes increasingly important for tissue characterization, sequence optimization and quantitative functional renal imaging, such as renal perfusion imaging using arterial spin labeling. By using a magnetization‐prepared single‐breath‐hold fast spin echo imaging method, human...

Read More thumbnail image: Measuring renal tissue relaxation times at 7 T

High‐performance liquid chromatographic enantioseparation of amino alcohol analogues possessing 1,2,3,4‐tetrahydroisoquinoline skeleton on polysaccharide‐based chiral stationary phases

Published: Oct 23, 2014

Author(s): Nóra Grecsó, István Ilisz, Zsanett Gecse, László Schönstein, Ferenc Fülöp, Antal Péter

Journal: Biomedical Chromatography

ABSTRACT The stereoisomers of 1,2,3,4‐tetrahydroisoquinoline amino alcohol analogues and derivatives thereof were separated in normal‐phase mode on chiral stationary phases based on preprepared silica coated with cellulose tris‐(3,5‐dimethylphenyl carbamate), cellulose tris‐(3‐chloro‐4‐methylphenyl carbamate), cellulose tris‐(4‐methylbenzoate) or cellulose tris‐(4‐chloro‐3‐methylphenyl carbamate). On all the investigated chiral columns, the retention and...

Read More thumbnail image: Highperformance liquid chromatographic enantioseparation of amino alcohol analogues possessing 1234tetrahydroisoquinoline skeleton on polysaccharidebased chiral stationary phases

Development and validation of a bioanalytical method by LC‐MS/MS for the quantification of the LAFIS 10 – an antimalarial candidate – and its pharmacokinetics first evaluation

Published: Oct 23, 2014

Author(s): J. V. Laureano, F. Barreto, S. Gnoatto, L. Tasso, T. Dalla Costa, B. V. Araujo

Journal: Biomedical Chromatography

ABSTRACT A rapid and highly sensitive method by LC‐MS/MS was developed and validated for the quantification of an antimalarial candidate (LAFIS10) in rat plasma using dexamethasone as internal standard (IS). The chromatographic separation was performed with a Poroshell 120 EC‐C18 column. The mobile phase consisted of water (A) and acetonitrile (B), both containing 10 mm of ammonium formate and 0.1% formic acid, delivered in the form of elution...

Read More thumbnail image: Development and validation of a bioanalytical method by LCMSMS for the quantification of the LAFIS 10  an antimalarial candidate  and its pharmacokinetics first evaluation

Generalized Dice's coefficient‐based multi‐block principal component analysis with Bayesian inference for plant‐wide process monitoring

Published: Oct 22, 2014

Author(s): Bei Wang, Xuefeng Yan, Qingchao Jiang, Zhaomin Lv

Journal: Journal of Chemometrics

Plant‐wide process monitoring is challenging because of the complex relationships among numerous variables in modern industrial processes. The multi‐block process monitoring method is an efficient approach applied to plant‐wide processes. However, dividing the original space into subspaces remains an open issue. The loading matrix generated by principal component analysis (PCA) describes the correlation between original variables and...

Read More thumbnail image: Generalized Dices coefficientbased multiblock principal component analysis with Bayesian inference for plantwide process monitoring

Use of specific polysaccharide‐immobilized monodisperse poly(glycidyl methacrylate) core–silica shell microspheres for affinity purification of lectins

Published: Oct 22, 2014

Author(s): Volodymyr Antonyuk, Silvia Grama, Zdeněk Plichta, Iryna Magorivska, Daniel Horak, Rostyslav Stoika

Journal: Biomedical Chromatography

ABSTRACT Immobilization of polysaccharides (yeast mannan and gum arabic) on the macroporous poly(glycidyl methacrylate) monodisperse microspheres coated with silica (SiO2)‐containing amino groups on the surface was used to prepare affinity sorbents for lectin purification. The efficiency of isolating mannose specific Pisum sativum lectin was demonstrated on sorbent with immobilized yeast mannan and that of galactose specific Glycine hispida...

Read More thumbnail image: Use of specific polysaccharideimmobilized monodisperse polyglycidyl methacrylate coresilica shell microspheres for affinity purification of lectins

Effects of American ginseng on pharmacokinetics of 5‐fluorouracil in rats

Published: Oct 22, 2014

Author(s): Yi‐Sheng He, Wei Sun, Chong‐Zhi Wang, Lian‐Wen Qi, Jie Yang, Ping Li, Xiao‐Dong Wen, Chun‐Su Yuan

Journal: Biomedical Chromatography

ABSTRACT The pharmacokinetics of 5‐fluorouracil (5‐FU) in combination with or without American ginseng (seven‐consecutive days oral dose) in rats were evaluated using liquid chromatography–electrospray ionization–mass spectrometry (LC‐MS). Chromatographic separation was performed on a reverse LC column within a total run time of 6.5 min, which allowed for a relatively quick analysis. The limit of quantification for 5‐FU was 15 ng/mL...

Read More thumbnail image: Effects of American ginseng on pharmacokinetics of 5fluorouracil in rats

Prestaining of glycoproteins in SDSPAGE via with 4H‐[1]‐Benzopyrano[4,3‐b]thiophene‐2‐carboxylic acid hydrazide with weak influence on protein mobility

Published: Oct 21, 2014

Author(s): Zhongxin Zhu, Xuan Zhou, Yang Wang, Qing Yu, Xinliang Zhu, Chao Niu, Weitao Cong, Litai Jin

Journal: ELECTROPHORESIS

A new fluorescent prestaining method for gel‐separated glycoproteins in 1D and 2D SDS‐PAGE was developed by using 4H‐[1]‐Benzopyrano[4,3‐b]thiophene‐2‐carboxylic acid hydrazide (BH). The prestained gels were readily imaged after electrophoresis without any time‐consuming steps needed for poststain. As low as 4–8 ng glycoproteins (transferrin, α1‐acid glycoprotein) could be selectively detected, which is comparable to the most commonly...

Read More thumbnail image: Prestaining of glycoproteins in SDS  PAGE via with 4H1Benzopyrano43bthiophene2carboxylic acid hydrazide with weak influence on protein mobility

Evaluation of the hepatotoxic risk caused by lead acetate via skin exposure using a proteomic approach

Published: Oct 21, 2014

Author(s): Jia‐You Fang, Pei‐Wen Wang, Chun‐Hsun Huang, Yi‐Yun Hung, Tai‐Long Pan

Journal: PROTEOMICS

Lead compounds exhibit a high degree of cytotoxicity and carcinogenicity. We evaluated the impact of lead acetate on the liver by skin exposure as well as the changes in protein profiles reflecting pathogenic processes. Functional proteomic tools showed that the most meaningful protein changes were involved in protein folding, ER stress, and apoptosis in the presence of an organic lead compound. Treatment with lead acetate also elicits...

Read More thumbnail image: Evaluation of the hepatotoxic risk caused by lead acetate via skin exposure using a proteomic approach

Comparison of the performance of different models for the interpretation of low level mixed DNA profiles

Published: Oct 21, 2014

Author(s): Todd W. Bille, Steven M. Weitz, Michael D. Coble, John Buckleton, Jo‐Anne Bright

Journal: ELECTROPHORESIS

DNA analyses from forensic casework samples commonly result in complex DNA profiles. Often, these profiles consist of multiple contributors and display multiple stochastic events such as peak height imbalance, allelic or locus drop‐out, allelic drop‐in, and excessive or indistinguishable stutter. This increased complexity has established a need for more sophisticated methods of DNA mixture interpretation. This study compares the...

Read More thumbnail image: Comparison of the performance of different models for the interpretation of low level mixed DNA profiles

Recent progress in analytical capillary isotachophoresis

Published: Oct 21, 2014

Author(s): Zdena Malá, Petr Gebauer, Petr Boček

Journal: ELECTROPHORESIS

This paper brings a survey of papers on analytical capillary ITP published since 2012 until the first quarter of 2014. These papers are ranged according to their nature, the techniques used, and the instrumentation employed. The sequence of the related chapter titles is as follows: Theory and simulations, techniques and instrumentation, single‐column and column‐switching applications of ITP, ITP in microfluidic systems, on‐line ITP‐CZE...

Read More thumbnail image: Recent progress in analytical capillary isotachophoresis

A simple technique to improve the resolution of membrane acidic proteins of the haloarchaeon Haloferax volcanii by 2D electrophoresis

Published: Oct 21, 2014

Author(s): Roberto A. Paggi, María Inés Giménez, Rosana E. De Castro, Andreina Cesari

Journal: ELECTROPHORESIS

Proteins present in the archaeal cell envelope play key roles in a variety of processes necessary for survival in extreme environments. The haloarchaeon Haloferax volcanii is a good model for membrane proteomic studies because its genome sequence is known, it can be genetically manipulated, and a number of studies at the “omics” level have been performed in this organism. This work reports an easy strategy to improve the resolution of...

Read More thumbnail image: A simple technique to improve the resolution of membrane acidic proteins of the haloarchaeon Haloferax volcanii by 2D electrophoresis

A serial dual‐electrode detector based on electrogenerated bromine for capillary electrophoresis

Published: Oct 21, 2014

Author(s): Fuying Du, Shunan Cao, Ying‐Sing Fung

Journal: ELECTROPHORESIS

A new serial dual‐electrode detector for CE has been designed and fabricated for postcolumn reaction detection based on electrogenerated bromine. A coaxial postcolumn reactor was employed to introduce bromide reagent and facilitate the fabrication of upstream generation electrode by simply sputtering Pt film onto the outer surface of the separation capillary. Bromide introduced could be efficiently converted to bromine at this Pt film...

Read More thumbnail image: A serial dualelectrode detector based on electrogenerated bromine for capillary electrophoresis

Quantification of heteroclitin D in rat plasma: validation of an LC/MS/MS method and its application in a preclinical pharmacokinetic study

Published: Oct 21, 2014

Author(s): Fan Zhang, Nan Zhang, Li Pang, Yan Tan, Hong Xu

Journal: Biomedical Chromatography

ABSTRACT A rapid, sensitive and specific liquid chromatography tandem mass spectrometry (LC/MS/MS) method was developed and validated for the quantification of heteroclitin D in rat plasma after using gambogic acid as internal standard (IS). Chromatographic separation was done on a Thermo Hypersil GOLD column (30 × 2.1 mm, 3 µm) using a mobile phase consisting of methanol–water–formic acid (80:20:0.1, v/v/v). The mass spectrometer...

Read More thumbnail image: Quantification of heteroclitin D in rat plasma validation of an LCMSMS method and its application in a preclinical pharmacokinetic study

Trace quantification of 1‐triacontanol in beagle plasma by GC‐MS/MS and its application to a pharmacokinetic study

Published: Oct 21, 2014

Author(s): Chunfeng Wang, Ali Fan, Xiaojie Zhu, Yang Lu, Shuhua Deng, Wenchao Gao, Wei Zhang, Qi Liu, Xijing Chen

Journal: Biomedical Chromatography

ABSTRACT 1‐Triacontanol (TA), a member of long chain fatty alcohol, has recently been received great attention owing to its antitumor activity. In this study, an accurate, sensitive and selective gas chromatography–tandem mass spectrometry method was developed and validated for the quantification of TA in beagle plasma using 1‐octacosanal as the internal standard (IS) for the first time. With temperature programming, chromatographic...

Read More thumbnail image: Trace quantification of 1triacontanol in beagle plasma by GCMSMS and its application to a pharmacokinetic study

Size‐based cell sorting with a resistive pulse sensor and an electromagnetic pump in a microfluidic chip

Published: Oct 21, 2014

Author(s): Yongxin Song, Mengqi Li, Xinxiang Pan, Qi Wang, Dongqing Li

Journal: ELECTROPHORESIS

An electrokinetic microfluidic chip is developed to detect and sort target cells by size from human blood samples. Target‐cell detection is achieved by a differential resistive pulse sensor (RPS) based on the size difference between the target cell and other cells. Once a target cell is detected, the detected RPS signal will automatically actuate an electromagnetic pump built in a microchannel to push the target cell into a collecting...

Read More thumbnail image: Sizebased cell sorting with a resistive pulse sensor and an electromagnetic pump in a microfluidic chip
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