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Determination of fusaric acid in maize using molecularly imprinted SPE clean‐up
Published: Dec 10, 2013
Author(s): Michael Appell, Michael A. Jackson, Lijuan C. Wang, Che‐Hin Ho, Anja Mueller
Journal: Journal of Separation Science
A new LC method to detect fusaric acid (FA) in maize is reported based on a molecularly imprinted SPE clean‐up using mimic‐templated molecularly imprinted polymers. Picolinic acid was used as a toxin analog for imprinting polymers during a thermolytic synthesis. Both acidic and basic functional monomers were predicted to have favorable binding interactions by MP2 ab initio calculations. Imprinted polymers synthesized with methacrylic acid or...
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SPE coupled with dispersive liquid–liquid microextraction followed by GC with flame ionization detection for the determination of ultra‐trace amounts of benzodiazepines
Published: Dec 9, 2013
Author(s): Masoomeh Ghobadi, Yadollah Yamini, Behnam Ebrahimpour
Journal: Journal of Separation Science
SPE combined with dispersive liquid–liquid microextration was used for the extraction of ultra‐trace amounts of benzodiazepines (BZPs) including, diazepam, midazolam, and alprazolam, from ultra‐pure water, tap water, fruit juices, and urine samples. The analytes were adsorbed from large volume samples (60 mL) onto octadecyl silica SPE columns. After the elution of the desired compounds from sorbents with 2.0 mL acetone, 0.5 mL of eluent...
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Ligand‐based design, virtual screening, and ADME/T‐based profiling on a dataset of 1,3,5‐triazine‐substituted benzene sulfonamides as carbonic anhydrase inhibitors
Published: Dec 9, 2013
Author(s): Amrita K. Saluja, Meena Tiwari, Surendra Kumar
Journal: Journal of Chemometrics
A quantitative structure–activity relationship (QSAR) analysis was performed on a dataset of 62 (1,3,5‐triazine‐substituted) benzene sulfonamides as carbonic anhydrase II and IX inhibitors using simulated annealing‐based multiple linear regression analysis. The selected QSAR model for carbonic anhydrase II inhibition (cross‐validated Q2 = 0.689, , ) showed that aromaticity, lipophilicity, electronegativity, and molecular projection in the XZ plane...
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Recombination analysis of autosomal short tandem repeats in C hinese H an families
Published: Dec 9, 2013
Author(s): Qiu‐Ling Liu, Hong Luo, Hu Zhao, Xiao‐Ling Huang, Jian‐Ding Cheng, De‐Jian Lu
Journal: ELECTROPHORESIS
Recombination fractions between forensic STRs can be extrapolated from the International HapMap Project, but the concordance between recombination fractions predicated from genetic maps and derived from observation of STR transmissions in families is still ambiguous for autosomal STRs because of limited family studies. Therefore, the main goal of this study is to compare recombination fractions estimated by pedigree analysis with those...
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CE analysis of single wood cells performing hydrolysis and preconcentration in open microchannels
Published: Dec 9, 2013
Author(s): Maria Khihon Rokhas, Saara Mikkonen, Juliane Beyer, Johan Jacksén, Åsa Emmer
Journal: ELECTROPHORESIS
In the present work, monosaccharides from pulp samples and single wood fibers were analyzed with CE, using indirect detection due to the lack of chromophores on the monosaccharides. The hydrolysis degradation of cellulose and hemicellulose into monosaccharides was performed using TFA, either in bulk scale or in microscale. In the microscale, one single wood fiber was hydrolyzed in an open microchannel manufactured on a silicon microchip...
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In‐line SPE ‐ CE using a fritless bead string design— A pplication for the analysis of organic sulfonates including inline SPE ‐ CE ‐ MS for APTS ‐labeled glycans
Published: Dec 9, 2013
Author(s): Kevin Jooß, Johannes Sommer, Svenja‐Catharina Bunz, Christian Neusüß
Journal: ELECTROPHORESIS
Despite many advantages like high separation efficiency CE comprises the main limitation of low concentration sensitivity, when compared to HPLC. In‐line SPE is an efficient way to increase concentration sensitivity. Here, a fritless in‐line‐SPE‐CE‐MS method was developed in order to analyze anions of strong acids. Mixed‐mode (weak anion exchange and RP) particles were used for enrichment and an acidic BGE was applied for separation....
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MRI of neonatal necrotizing enterocolitis in a rodent model
Published: Dec 8, 2013
Author(s): Devkumar Mustafi, Sheng‐Ru Shiou, Xiaobing Fan, Erica Markiewicz, Gregory S. Karczmar, Erika C. Claud
Journal: NMR in Biomedicine
Neonatal necrotizing enterocolitis (NEC) is a poorly understood life‐threatening illness afflicting premature infants. Research is hampered by the absence of a suitable method to monitor disease progression noninvasively. The primary goal of this research was to test in vivo MRI methods for the noninvasive early detection and staging of inflammation in the ileum of an infant rat model of NEC. Neonatal rats were delivered by cesarean...
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Determination of rice type by 1 H NMR spectroscopy in combination with different chemometric tools
Published: Dec 8, 2013
Author(s): Yulia B. Monakhova, Douglas N. Rutledge, Andreas Roßmann, Hans‐Ulrich Waiblinger, Manuela Mahler, Maren Ilse, Thomas Kuballa, Dirk W. Lachenmeier
Journal: Journal of Chemometrics
A 400‐MHz 1H nuclear magnetic resonance (NMR) spectroscopy and multivariate data analysis were used in the context of food surveillance to discriminate 46 authentic rice samples according to type. It was found that the optimal sample preparation consists of preparing aqueous rice extracts at pH 1.9. For the first time, the chemometric method independent component analysis (ICA) was applied to differentiate clusters of rice from the same...
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Metabolism of aildenafil in vivo in rats and in vitro in mouse, rat, dog, and human liver microsomes
Published: Dec 6, 2013
Author(s): Yan Li, Linan Wu, Yuan Gu, Duanyun Si, Changxiao Liu
Journal: Drug Testing and Analysis
Aildenafil, 1‐{[3‐(6, 7‐dihydro‐1‐methyl‐7‐oxo‐3‐propyl‐1H‐pyrazolo [4, 3‐d] primidin‐5‐yl)‐4‐ethoxyphenyl] sulfonyl}‐cis‐3, 5‐dimethylpiperazine, a phosphodiesterase type V enzyme inhibitor (PDE5I), is under development for treatment of erectile dysfunction (ED). The purpose of this study was to elucidate metabolism of aildenafil in vivo in rats and in vitro in mouse, rat, dog, and human liver microsomes. Thirty‐one phase I metabolites have been found by...
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Isolation and purification of recombinant human plasminogen Kringle 5 by liquid chromatography and ammonium sulfate salting‐out
Published: Dec 6, 2013
Author(s): Liujiao Bian, Xu Ji, Wei Hu
Journal: Biomedical Chromatography
ABSTRACT In this work, a novel method was established to isolate and purify Human plasminogen Kringle 5 (HPK5) as a histidine‐tagged fusion protein expressed in Escherichia coli BL21 (DE3). This method consisted of sample extraction using a Ni‐chelated Sepharose Fast‐Flow affinity column, ammonium sulfate salting‐out and Sephadex G‐75 size‐exclusion column in turn. The purity analysis by SDS–PAGE, high‐performance size‐exclusion and...
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Simultaneous determination of corosolic acid and euscaphic acid in the plasma of normal and diabetic rat after oral administration of extract of Potentilla discolor Bunge by high‐performance liquid chromatography/electrospray ionization mass spect
Published: Dec 6, 2013
Author(s): Jing‐jing Li, Yi Li, Min Bai, Jing‐fu Tan, Qiang Wang, Jie Yang
Journal: Biomedical Chromatography
ABSTRACT Potentilla discolor Bunge has been used for diabetes in China for a long time. Corosolic acid (CA) and euscaphic acid (EA), with significant anti‐diabetic activity, are two major triterpenoids in P. discolor. In this study, a specific, sensitive and convenient LC‐MS method has been developed for simultaneous determination of CA and EA in the plasma of normal and diabetic rats after oral administration of the extract of P. discolor. The...
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Data and device protection: A ToF‐SIMS, wetting, and XPS study of an Apple iPod nano
Published: Dec 6, 2013
Author(s): Vipul Gupta, Joshua A. Tuscano, Naomi R. Romriell, Robert C. Davis, Matthew R. Linford
Journal: Surface and Interface Analysis
An important aspect of the robustness of an electronic device is its ability to resist water, fingerprints, dirt, and smudges that may compromise its ability to function and/or the information within it. Here, we report a chemical analysis by ToF‐SIMS, wetting, and XPS of the surfaces in a commercially available Apple iPod nano (8GB, MC525LL/A), which showed good resistance to its environment. This analysis reveals that the front panel...
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Analysis of vitamin D metabolic markers by mass spectrometry: Current techniques, limitations of the “gold standard” method, and anticipated future directions
Published: Dec 6, 2013
Author(s): Dietrich A. Volmer, Luana R.B.C. Mendes, Caroline S. Stokes
Journal: Mass Spectrometry Reviews
Abstract Vitamin D compounds belong to a group of secosteroids, which occur naturally as vitamin D3 in mammals and D2 in plants. Vitamin D is vital for bone health but recent studies have shown a much wider role in the pathologies of diseases such as diabetes, cancer, autoimmune, neurodegenerative, mental and cardiovascular diseases. Photosynthesis of vitamin D in the human skin and subsequent hepatic and renal metabolism...
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M ass spectrometry analysis of nitrotyrosine‐containing proteins
Published: Dec 6, 2013
Author(s): Xianquan Zhan, Xiaowei Wang, Dominic M. Desiderio
Journal: Mass Spectrometry Reviews
Abstract Oxidative stress plays important roles in a wide range of diseases such as cancer, inflammatory disease, neurodegenerative disorders, etc. Tyrosine nitration in a protein is a chemically stable oxidative modification, and a marker of oxidative injuries. Mass spectrometry (MS) is a key technique to identify nitrotyrosine‐containing proteins and nitrotyrosine sites in endogenous and synthetic...
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Recent advances in coupling capillary electrophoresis‐based separation techniques to ESI and MALDI‐MS
Published: Dec 5, 2013
Author(s): Xuefei Zhong, Zichuan Zhang, Shan Jiang, Lingjun Li
Journal: ELECTROPHORESIS
Coupling CE‐based separation techniques to MS creates a powerful platform for analysis of a wide range of biomolecules from complex samples because it combines the high separation efficiency of CE and the sensitivity and selectivity of MS detection. ESI and MALDI, as the most common soft ionization techniques employed for CE and MS coupling, offer distinct advantages for biomolecular characterization. This review is focused primarily on...
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Rapid and sensitive detection of lower respiratory tract infections by stuffer‐free multiplex ligation‐dependent probe amplification
Published: Dec 5, 2013
Author(s): Boram Chung, Gi Won Shin, Chan Kwon Park, Woong Choi, Yeun‐Jun Chung, Hyung Kyu Yoon, Gyoo Yeol Jung
Journal: ELECTROPHORESIS
Lower respiratory tract infection is one of the most common infectious diseases. However, conventional methods for detecting infectious pathogens are time‐consuming, and generally have a limited impact on early therapeutic decisions. We previously reported a rapid and sensitive method for detecting such pathogens using stuffer‐free multiplex ligation‐dependent probe amplification coupled with high‐resolution CE‐SSCP. In this study, we...
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A simple, one‐tube assay for the simultaneous detection and diagnosis of ten A ustralian poultry E imeria
Published: Dec 5, 2013
Author(s): Rosamond M. Godwin, Jess A. T. Morgan
Journal: ELECTROPHORESIS
Coccidiosis is a costly worldwide enteric disease of chickens caused by parasites of the genus Eimeria. At present, there are seven described species that occur globally and a further three undescribed, operational taxonomic units (OTUs X, Y, and Z) that are known to infect chickens from Australia. Species of Eimeria have both overlapping morphology and pathology and frequently occur as mixed‐species infections. This makes definitive diagnosis with...
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Continuous flow nanoparticle concentration using alternating current‐electroosmotic flow
Published: Dec 5, 2013
Author(s): Kai F. Hoettges, Martin B. McDonnell, Michael P. Hughes
Journal: ELECTROPHORESIS
Achieving real‐time detection of environmental pathogens such as viruses and bacterial spores requires detectors with both rapid action and a suitable detection threshold. However, most biosensors have detection limits of an order of magnitude or more above the potential infection threshold, limiting their usefulness. This can be improved through the use of automated sample preparation techniques such as preconcentration. In this paper,...
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Dynamic microparticle manipulation with an electroosmotic flow gradient in low‐frequency alternating current dielectrophoresis
Published: Dec 5, 2013
Author(s): Aytug Gencoglu, David Olney, Alexandra LaLonde, Karuna S. Koppula, Blanca H. Lapizco‐Encinas
Journal: ELECTROPHORESIS
In this study, the potential of low‐frequency AC insulator‐based DEP (iDEP) was explored for the separation of polystyrene microparticles and yeast cells. An EOF gradient was generated by employing an asymmetrical, 20 Hz AC electrical signal in an iDEP device consisting of a microchannel with diamond‐shaped insulating posts. Two types of samples were analyzed, the first sample contained three types of polystyrene particles with different...
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Efficient algorithm for simulation of isoelectric focusing
Published: Dec 5, 2013
Author(s): Kisoo Yoo, Jaesool Shim, Jin Liu, Prashanta Dutta
Journal: ELECTROPHORESIS
IEF simulation is an effective tool to investigate the transport phenomena and separation performance as well as to design IEF microchip. However, multidimensional IEF simulations are computationally intensive as one has to solve a large number of mass conservation equations for ampholytes to simulate a realistic case. In this study, a parallel scheme for a 2D IEF simulation is developed to reduce the computational time. The calculation...
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