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Prestaining of glycoproteins in SDSPAGE via with 4H‐[1]‐Benzopyrano[4,3‐b]thiophene‐2‐carboxylic acid hydrazide with weak influence on protein mobility

Published: Oct 21, 2014

Author(s): Zhongxin Zhu, Xuan Zhou, Yang Wang, Qing Yu, Xinliang Zhu, Chao Niu, Weitao Cong, Litai Jin

Journal: ELECTROPHORESIS

A new fluorescent prestaining method for gel‐separated glycoproteins in 1D and 2D SDS‐PAGE was developed by using 4H‐[1]‐Benzopyrano[4,3‐b]thiophene‐2‐carboxylic acid hydrazide (BH). The prestained gels were readily imaged after electrophoresis without any time‐consuming steps needed for poststain. As low as 4–8 ng glycoproteins (transferrin, α1‐acid glycoprotein) could be selectively detected, which is comparable to the most commonly...

Read More thumbnail image: Prestaining of glycoproteins in SDS  PAGE via with 4H1Benzopyrano43bthiophene2carboxylic acid hydrazide with weak influence on protein mobility

Evaluation of the hepatotoxic risk caused by lead acetate via skin exposure using a proteomic approach

Published: Oct 21, 2014

Author(s): Jia‐You Fang, Pei‐Wen Wang, Chun‐Hsun Huang, Yi‐Yun Hung, Tai‐Long Pan

Journal: PROTEOMICS

Lead compounds exhibit a high degree of cytotoxicity and carcinogenicity. We evaluated the impact of lead acetate on the liver by skin exposure as well as the changes in protein profiles reflecting pathogenic processes. Functional proteomic tools showed that the most meaningful protein changes were involved in protein folding, ER stress, and apoptosis in the presence of an organic lead compound. Treatment with lead acetate also elicits...

Read More thumbnail image: Evaluation of the hepatotoxic risk caused by lead acetate via skin exposure using a proteomic approach

Comparison of the performance of different models for the interpretation of low level mixed DNA profiles

Published: Oct 21, 2014

Author(s): Todd W. Bille, Steven M. Weitz, Michael D. Coble, John Buckleton, Jo‐Anne Bright

Journal: ELECTROPHORESIS

DNA analyses from forensic casework samples commonly result in complex DNA profiles. Often, these profiles consist of multiple contributors and display multiple stochastic events such as peak height imbalance, allelic or locus drop‐out, allelic drop‐in, and excessive or indistinguishable stutter. This increased complexity has established a need for more sophisticated methods of DNA mixture interpretation. This study compares the...

Read More thumbnail image: Comparison of the performance of different models for the interpretation of low level mixed DNA profiles

Recent progress in analytical capillary isotachophoresis

Published: Oct 21, 2014

Author(s): Zdena Malá, Petr Gebauer, Petr Boček

Journal: ELECTROPHORESIS

This paper brings a survey of papers on analytical capillary ITP published since 2012 until the first quarter of 2014. These papers are ranged according to their nature, the techniques used, and the instrumentation employed. The sequence of the related chapter titles is as follows: Theory and simulations, techniques and instrumentation, single‐column and column‐switching applications of ITP, ITP in microfluidic systems, on‐line ITP‐CZE...

Read More thumbnail image: Recent progress in analytical capillary isotachophoresis

A simple technique to improve the resolution of membrane acidic proteins of the haloarchaeon Haloferax volcanii by 2D electrophoresis

Published: Oct 21, 2014

Author(s): Roberto A. Paggi, María Inés Giménez, Rosana E. De Castro, Andreina Cesari

Journal: ELECTROPHORESIS

Proteins present in the archaeal cell envelope play key roles in a variety of processes necessary for survival in extreme environments. The haloarchaeon Haloferax volcanii is a good model for membrane proteomic studies because its genome sequence is known, it can be genetically manipulated, and a number of studies at the “omics” level have been performed in this organism. This work reports an easy strategy to improve the resolution of...

Read More thumbnail image: A simple technique to improve the resolution of membrane acidic proteins of the haloarchaeon Haloferax volcanii by 2D electrophoresis

A serial dual‐electrode detector based on electrogenerated bromine for capillary electrophoresis

Published: Oct 21, 2014

Author(s): Fuying Du, Shunan Cao, Ying‐Sing Fung

Journal: ELECTROPHORESIS

A new serial dual‐electrode detector for CE has been designed and fabricated for postcolumn reaction detection based on electrogenerated bromine. A coaxial postcolumn reactor was employed to introduce bromide reagent and facilitate the fabrication of upstream generation electrode by simply sputtering Pt film onto the outer surface of the separation capillary. Bromide introduced could be efficiently converted to bromine at this Pt film...

Read More thumbnail image: A serial dualelectrode detector based on electrogenerated bromine for capillary electrophoresis

Quantification of heteroclitin D in rat plasma: validation of an LC/MS/MS method and its application in a preclinical pharmacokinetic study

Published: Oct 21, 2014

Author(s): Fan Zhang, Nan Zhang, Li Pang, Yan Tan, Hong Xu

Journal: Biomedical Chromatography

ABSTRACT A rapid, sensitive and specific liquid chromatography tandem mass spectrometry (LC/MS/MS) method was developed and validated for the quantification of heteroclitin D in rat plasma after using gambogic acid as internal standard (IS). Chromatographic separation was done on a Thermo Hypersil GOLD column (30 × 2.1 mm, 3 µm) using a mobile phase consisting of methanol–water–formic acid (80:20:0.1, v/v/v). The mass spectrometer...

Read More thumbnail image: Quantification of heteroclitin D in rat plasma validation of an LCMSMS method and its application in a preclinical pharmacokinetic study

Trace quantification of 1‐triacontanol in beagle plasma by GC‐MS/MS and its application to a pharmacokinetic study

Published: Oct 21, 2014

Author(s): Chunfeng Wang, Ali Fan, Xiaojie Zhu, Yang Lu, Shuhua Deng, Wenchao Gao, Wei Zhang, Qi Liu, Xijing Chen

Journal: Biomedical Chromatography

ABSTRACT 1‐Triacontanol (TA), a member of long chain fatty alcohol, has recently been received great attention owing to its antitumor activity. In this study, an accurate, sensitive and selective gas chromatography–tandem mass spectrometry method was developed and validated for the quantification of TA in beagle plasma using 1‐octacosanal as the internal standard (IS) for the first time. With temperature programming, chromatographic...

Read More thumbnail image: Trace quantification of 1triacontanol in beagle plasma by GCMSMS and its application to a pharmacokinetic study

Size‐based cell sorting with a resistive pulse sensor and an electromagnetic pump in a microfluidic chip

Published: Oct 21, 2014

Author(s): Yongxin Song, Mengqi Li, Xinxiang Pan, Qi Wang, Dongqing Li

Journal: ELECTROPHORESIS

An electrokinetic microfluidic chip is developed to detect and sort target cells by size from human blood samples. Target‐cell detection is achieved by a differential resistive pulse sensor (RPS) based on the size difference between the target cell and other cells. Once a target cell is detected, the detected RPS signal will automatically actuate an electromagnetic pump built in a microchannel to push the target cell into a collecting...

Read More thumbnail image: Sizebased cell sorting with a resistive pulse sensor and an electromagnetic pump in a microfluidic chip

Extraction of methocarbamol from human plasma with a polypyrrole/multiwalled carbon nanotubes composite decorated with magnetic nanoparticles as an adsorbent followed by electrospray ionization ion mobility spectrometry detection

Published: Oct 21, 2014

Author(s): Mohammad Saraji, Taghi Khayamian, Zahra Hashemian

Journal: Journal of Separation Science

In this work, a polypyrrole/multiwalled carbon nanotubes composite decorated with Fe3O4 nanoparticles was chemically synthesized and applied as a novel adsorbent for the extraction of methocarbamol from human plasma. Electrospray ionization ion mobility spectrometry was used for the determination of the analyte. The properties of the magnetic‐modified adsorbent were characterized by scanning electron microscopy, transmission electron microscopy,...

Read More thumbnail image: Extraction of methocarbamol from human plasma with a polypyrrolemultiwalled carbon nanotubes composite decorated with magnetic nanoparticles as an adsorbent followed by electrospray ionization ion mobility spectrometry detection

C 18 ‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance

Published: Oct 21, 2014

Author(s): Faiz Ali, Won Jo Cheong

Journal: Journal of Separation Science

Ground porous silica monolith particles with an average particle size of 2.34 μm and large pores (363 Å) exhibiting excellent chromatographic performance have been synthesized on a relatively large scale by a sophisticated sol–gel procedure. The particle size distribution was rather broad, and the d(0.1)/d(0.9) ratio was 0.14. The resultant silica monolith particles were chemically modified with chlorodimethyloctadecylsilane and...

Read More thumbnail image: C 18 bound porous silica monolith particles as a lowcost highperformance liquid chromatography stationary phase with an excellent chromatographic performance

Assessment of teeth elemental content using μ ‐EDXRF: effects by in‐office and at‐home bleaching products

Published: Oct 20, 2014

Author(s): J. Silveira, J. Godinho, A. Mata, M. L. Carvalho, S. Pessanha

Journal: X-Ray Spectrometry

The purpose of this in vitro study is to assess whether the elemental content of tooth enamel is altered when bleaching the teeth with bleaching gel. Because of tooth bleaching growing popularity and to match the public demands, manufacturers increased the range of bleaching products available, changing its presentation, techniques and concentration of active principles. This way, two different bleaching products were compared, one for...

Read More thumbnail image: Assessment of teeth elemental content using  EDXRF effects by inoffice and athome bleaching products

Design of guanidinium ionic liquid based microwave‐assisted extraction for the efficient extraction of Praeruptorin A from Radix peucedani

Published: Oct 20, 2014

Author(s): Xueqin Ding, Li Li, Yuzhi Wang, Jing Chen, Yanhua Huang, Kaijia Xu

Journal: Journal of Separation Science

A series of novel tetramethylguanidinium ionic liquids and hexaalkylguanidinium ionic liquids have been synthesized based on 1,1,3,3‐tetramethylguanidine. The structures of the ionic liquids were confirmed by 1H NMR spectroscopy and mass spectrometry. A green guanidinium ionic liquid based microwave‐assisted extraction method has been developed with these guanidinium ionic liquids for the effective extraction of Praeruptorin A from Radix peucedani....

Read More thumbnail image: Design of guanidinium ionic liquid based microwaveassisted extraction for the efficient extraction of Praeruptorin A from Radix peucedani

High‐resolution melt analysis of the minisatellite D1S80: A potential forensic screening tool

Published: Oct 20, 2014

Author(s): Robert S. Pomeroy, Kuppareddi Balamurugan, Helena Wong, George Duncan

Journal: ELECTROPHORESIS

High‐resolution melt (HRM) analysis of the VNTR region of the human D1S80 locus, a 16‐bp repeat minisatellite from approximately 400 to over 700 bp in length, was investigated. A Qiagen Rotor‐Gene Q using the Type‐it PCR HRM kit was used to acquire HRM curves for 14 single, and 16 biallelic, dsDNA samples. The HRM analysis was applicable over a range of DNA concentrations; however the characteristics of the melt curve did depend on the...

Read More thumbnail image: Highresolution melt analysis of the minisatellite D1S80 A potential forensic screening tool

Optimization of ELFSE DNA sequencing with EOF counterflow and microfluidics

Published: Oct 20, 2014

Author(s): Max A. Fahrenkopf, Tamal Mukherjee, B. Erik Ydstie, James W. Schneider

Journal: ELECTROPHORESIS

We present a nonlinear optimization study of different implementations of the DNA electrophoretic method “End‐labeled Free‐solution Electrophoresis” in commercial capillary electrophoresis systems and microfluidics to improve the time required for readout. Here, the effect of electro‐osmotic counterflows and snap‐shot detection are considered to allow for detection of peaks soon after they are electorphoretically resolved. Using drag tags...

Read More thumbnail image: Optimization of ELFSE DNA sequencing with EOF counterflow and microfluidics

Separation of natural antioxidants using PDMS electrophoresis microchips coupled with amperometric detection and reverse polarity

Published: Oct 20, 2014

Author(s): Bruno Gabriel Lucca, Susan Marie Lunte, Wendell Karlos Tomazelli Coltro, Valdir Souza Ferreira

Journal: ELECTROPHORESIS

This report describes the use of PDMS ME coupled with amperometric detection for rapid separation of ascorbic, gallic , ferulic, p‐coumaric acids using reverse polarity. ME devices were fabricated in PDMS by soft lithography and detection was accomplished using an integrated carbon fiber working electrode aligned in the end‐channel configuration. Separation and detection parameters were investigated and the best conditions were obtained...

Read More thumbnail image: Separation of natural antioxidants using PDMS electrophoresis microchips coupled with amperometric detection and reverse polarity

Enantioseparation of aldols by high‐performance liquid chromatography on polysaccharide‐based chiral stationary phases that bear chlorinated substituents

Published: Oct 20, 2014

Author(s): Sonia Pedotti, Angela Patti

Journal: Journal of Separation Science

Two families of aldols, obtained from the condensation of aromatic aldehydes with cyclohexanone or acetone (ten examples in each group), were analyzed by high‐performance liquid chromatography in normal phase elution mode on three polysaccharide‐based chiral stationary phases of the Lux series, namely, Lux Cellulose‐2, Lux Cellulose‐4 and Lux Amylose‐2, which share the common feature of chlorinated substituents in the chiral selectors....

Read More thumbnail image: Enantioseparation of aldols by highperformance liquid chromatography on polysaccharidebased chiral stationary phases that bear chlorinated substituents

F‐108 polymer and capillary electrophoresis easily resolves complex environmental DNA mixtures and SNPs

Published: Oct 20, 2014

Author(s): Natalie Damaso, Lauren Martin, Priyanka Kushwaha, DeEtta Mills

Journal: ELECTROPHORESIS

Ecological studies of microbial communities often use profiling methods but the true community diversity can be underestimated in methods that separate amplicons based on sequence length using performance optimized polymer 4. Taxonomically, unrelated organisms can produce the same length amplicon even though the amplicons have different sequences. F‐108 polymer has previously been shown to resolve same length amplicons by sequence...

Read More thumbnail image: F108 polymer and capillary electrophoresis easily resolves complex environmental DNA mixtures and SNPs

Alternating current cloud point extraction on a microchip: The effect of electrode geometry

Published: Oct 20, 2014

Author(s): Naoki Sasaki, Chisaki Maekawa, Kae Sato

Journal: ELECTROPHORESIS

We report on the effect of electrode geometry on alternating current cloud point extraction (ACPE). ACPE is a technique utilized to extract membrane‐associated biomolecules in an electrode‐integrated microfluidic channel. In this study, we investigated the effect of gap size (4∼22 μm) between microband electrodes on ACPE. A decrease in gap size resulted in efficient and rapid concentration of fluorescent‐labeled phospholipids, a model of...

Read More thumbnail image: Alternating current cloud point extraction on a microchip The effect of electrode geometry

Possible cause of lack of positive samples on homologous blood transfusion

Published: Oct 20, 2014

Author(s): Grigory Krotov, Maria Nikitina, Grigory Rodchenkov

Journal: Drug Testing and Analysis

Homologous blood transfusion is a prohibited method of blood manipulation that can be used to increase the number of erythrocytes circulating in the blood stream resulting in an increased oxygen transport capacity. In doping controls, homologous blood transfusions are determined by means of a procedure based on the detection of red blood cell phenotypes by flow cytometry. In the past six years, no adverse analytical findings concerning...

Read More thumbnail image: Possible cause of lack of positive samples on homologous blood transfusion
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