EarlyView Articles

Discrimination of Polygoni Multiflori radix and Cynanchi Auriculati radix using ultra‐high performance liquid chromatography fingerprints and chemical pattern recognition

Published: Aug 18, 2017

Author(s): Lili Sun, Meng Wang, Yali Liu, Huijie Zhang, Yanan Liu, Xiaoliang Ren, Yanru Deng

Journal: Biomedical Chromatography

Abstract In this work, a strategy was proposed to discriminate Polygoni Multiflori Radix (PMR) and its adulterant (Cynanchi Auriculati Radix, CAR). Ultra‐high performance liquid chromatography (UHPLC) fingerprints were established to analyze samples containing PMR, CAR and mixtures simultaneously. Multivariate classification methods were applied to analyze the obtained UHPLC fingerprints, including principal component analysis...

Screening and identification of metabolites of two kinds of main active ingredients and hepatotoxic pyrrolizidine alkaloids in rat after lavage Farfarae Flos extract by UHPLC‐Q‐TOF‐MS mass spectrometry

Published: Aug 18, 2017

Author(s): Xiaoye Cheng, Man Liao, Xinpeng Diao, Yupeng Sun, Lantong Zhang

Journal: Biomedical Chromatography

Abstract Farfarae Flos, the dried flower buds of Tussilago farfara L., is usually used to treat coughs, bronchitic and asthmatic conditions as an important traditional Chinese medicine. Tussilagone and methl butyric acid tussilagin ester are seen as representatives of two kinds of active substances. In addition, the pyrrolizidine alkaloids, mainly senkirkine and senecionine, present in the herb can be hepatoxic. In this study, a rapid and...

Characterization of ashes from greenhouse crops plant biomass residues using X‐ray fluorescence analysis and X‐ray diffraction

Published: Aug 17, 2017

Author(s): Eduardo Garzón, Laura Morales, José M. Martínez‐Blanes, Pedro J. Sánchez‐Soto

Journal: X-Ray Spectrometry

A characterization of ashes obtained by thermal treatments on greenhouse crops plant biomass residues is presented. The chemical analysis, by X‐ray fluorescence (wavelength‐dispersive X‐ray fluorescence), and phase analysis, by X‐ray diffraction, of the resultant ashes are reported. Thermal treatments of selected samples of these residues increase the relative amounts of inorganic Mg, Si, P, and S in the ashes, being these amounts as high...

Simultaneous determination and method validation of difenoconazole, propiconazole and pyraclostrobin in pepper and soil by LC–MS/MS in field trial samples from three provinces, China

Published: Aug 17, 2017

Author(s): Sizhuo Wu, Haizhen Zhang, Kunming Zheng, Banghua Meng, Fei Wang, Ying Cui, Song Zeng, Kankan Zhang, Deyu Hu

Journal: Biomedical Chromatography

Abstract A liquid chromatography–electrospray ionization tandem mass spectrometry method was developed for simple and accurate detection of the fungicides difenoconazole, propiconazole and pyraclostrobin in peppers and soil. Three fungicides residues were extracted from samples by acetonitrile and cleaned up by dispersive solid‐phase extraction before instrumental analysis. The accuracy and precision of the method were evaluated by...

Selectivity‐relaxed classical and inverse least squares calibration and selectivity measures with a unified selectivity coefficient

Published: Aug 17, 2017

Author(s): John H. Kalivas, Joan Ferré, Alister J. Tencate

Journal: Journal of Chemometrics

Two popular calibration strategies are classical least squares (CLS) and inverse least squares (ILS). Underlying CLS is that the net analyte signal used for quantitation is orthogonal to signal from other components (interferents). The CLS orthogonality avoids analyte prediction bias from modeled interferents. Although this orthogonality condition ensures full analyte selectivity, it may increase the mean squared error of prediction....

Advances in equine anti‐doping

Published: Aug 16, 2017

Author(s): Adam Cawley

Journal: Drug Testing and Analysis

An improved model for malaria pigment and β ‐hematin: Fe(OEP)picrate

Published: Aug 16, 2017

Author(s): Ratchadaporn Puntharod, Kenneth J. Haller, Evan G. Robertson, Eunice S. H. Gwee, Ekaterina I. Izgorodina, Bayden R. Wood

Journal: Journal of Raman Spectroscopy

Synthesis and crystallographic and spectroscopic structural characterization, along with spectral band assignments calculated by using density functional theory (M06L/cc‐pVDZ and B3LYP/6‐31G(d)), are reported for Fe(OEP)picrate as a model for hemozoin (malaria pigment) and its synthetic analog β‐hematin, which are spectroscopically identical. The average Fe–N distance, 2.044(12) Å, and the Raman modes indicate a high‐spin ...

Optimization of recombinant β‐glucuronidase hydrolysis and quantification of eight urinary cannabinoids and metabolites by liquid chromatography tandem mass spectrometry

Published: Aug 16, 2017

Author(s): Cristina Sempio, Karl B. Scheidweiler, Allan J. Barnes, Marilyn A. Huestis

Journal: Drug Testing and Analysis

Prolonged urinary cannabinoid excretion in chronic frequent cannabis users confounds identification of recent cannabis intake that may be important in treatment, workplace, clinical, and forensic testing programs. In addition, differentiation of synthetic Δ9‐tetrahydrocannabinol (THC) intake from cannabis plant products might be an important interpretive issue. THC, 11‐hydroxy‐THC (11‐OH‐THC) and 11‐nor‐9‐carboxy‐THC (THCCOOH) urine...

Investigation of drug products received for analysis in the Swedish STRIDA project on new psychoactive substances

Published: Aug 16, 2017

Author(s): Matilda Bäckberg, Karl‐Henrik Jönsson, Olof Beck, Anders Helander

Journal: Drug Testing and Analysis

The web‐based open sale of unregulated new psychoactive substances (NPS) has shown a steady increase in recent years. Analysis of drug products sold as NPS is useful to confirm the true chemical contents, for comparison with the substances detected in corresponding body fluids, but also to study drug trends. This work describes the examination of 251 drug products that were randomly submitted for analysis in 173 cases of suspected...

Development and validation of a high‐performance liquid chromatography method for the quantification of talazoparib in rat plasma: Application to plasma protein binding studies

Published: Aug 15, 2017

Author(s): Mahendra Kumar Hidau, Srikanth Kolluru, Srinath Palakurthi

Journal: Biomedical Chromatography

Abstract A sensitive and selective RP‐HPLC method has been developed and validated for the quantification of a highly potent poly ADP ribose polymerase inhibitor talazoparib (TZP) in rat plasma. Chromatographic separation was performed with isocratic elution method. Absorbance for TZP was measured with a UV detector (SPD‐20A UV–vis) at a λmax of 227 nm. Protein precipitation was used to extract the drug from plasma samples using methanol–acetonitrile...

Application of headspace solid‐phase microextraction followed by gas chromatography coupled with mass spectrometry to determine esters of carboxylic acids and other volatile compounds in Dermestes maculatus and Dermestes ater lipids

Published: Aug 15, 2017

Author(s): Magdalena Cerkowniak, Mieczysława I. Boguś, Emilia Włóka, Piotr Stepnowski, Marek Gołębiowski

Journal: Biomedical Chromatography

Abstract A constant problem in veterinary medicine, human healthcare, agriculture, forestry and horticulture is the large number of pests, and the lack of effective methods to combat them which cause no harm to the rest of the environment. It is recommended and desired to reduce the use of chemicals and increase the use of agents based on knowledge acquired in the fields of biology, chemistry and agrochemicals. To learn the defense...

Characterizing hypoxia in human glioma: A simultaneous multimodal MRI and PET study

Published: Aug 14, 2017

Author(s): Christine Preibisch, Kuangyu Shi, Anne Kluge, Mathias Lukas, Benedikt Wiestler, Jens Göttler, Jens Gempt, Florian Ringel, Mohamed Al Jaberi, Jürgen Schlegel, Bernhard Meyer, Claus Zimmer, Thomas Pyka, Stefan Förster

Journal: NMR in Biomedicine

Hypoxia plays an important role for the prognosis and therapy response of cancer. Thus, hypoxia imaging would be a valuable tool for pre‐therapeutic assessment of tumor malignancy. However, there is no standard validated technique for clinical application available yet. Therefore, we performed a study in 12 patients with high‐grade glioma, where we directly compared the two currently most promising techniques, namely the MR‐based relative...

Chemical exchange saturation transfer imaging Advances and applications_ M_ T_ McMahon A_ A_ Gilad J_ W_ M_ Bulte P_ C_ M_ van Zijl Pan Stanford Publishing Pte_ Ltd_ Singapore 2017 179_95 Hardcover and 125_97 Ebook pp_ 496

Published: Aug 14, 2017

Author(s): Peter Bachert

Journal: NMR in Biomedicine

Chromatographic HPV‐16 E6/E7 plasmid vaccine purification employing L‐histidine and 1‐benzyl‐L‐histidine affinity ligands

Published: Aug 14, 2017

Author(s): Lúcia F. A. Amorim, Rita Gaspar, Patrícia Pereira, Urh Černigoj, Fani Sousa, João António Queiroz, Ângela Sousa

Journal: ELECTROPHORESIS

Affinity chromatography based on amino acids as interacting ligands was already indicated as an alternative compared to ion exchange or hydrophobic interaction for plasmid DNA purification. Understanding the recognition mechanisms occurring between histidine‐based ligands and nucleic acids enables more efficient purification of a DNA vaccine, as the binding and elution conditions can be adjusted in order to enhance the purification...

Silver nanoparticles functionalized with ampicillin

Published: Aug 14, 2017

Author(s): Agnieszka Rogowska, Katarzyna Rafińska, Paweł Pomastowski, Justyna Walczak, Viorica Railean‐Plugaru, Magdalena Buszewska‐Forajta, Bogusław Buszewski

Journal: ELECTROPHORESIS

The resistance of pathogenic bacteria to antibiotics has become a serious problem. The emphasis is placed on the development of new, effective antimicrobial strategies. One of them is the use of AgNPs in association with antibiotic drugs. The aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high‐performance liquid...

Checking transfer efficiency and equal loading via qualitative optical way in western blotting

Published: Aug 14, 2017

Author(s): Jun‐Hua Gong, Jian‐Ping Gong, Kai‐Wen Zheng

Journal: ELECTROPHORESIS

The ability to determine that successful transfer and equal loading occur prior to using primary antibodies is important. And total protein staining is commonly used to check transfer efficiency and normalization, which play a crucial role in western blotting. Ponceau S and coomassie blue are commonly used, but there are disadvantages reported in recent years. Therefore, we are interested in finding another method, which is cheap, easy...

Phytic acid induced three‐dimensional graphene for the enrichment of phthalate esters from bottled water and sports beverage samples

Published: Aug 14, 2017

Author(s): Xinyu Liang, Juntao Wang, Juanjuan Wu, Qiuhua Wu, Chun Wang, Zhi Wang

Journal: Journal of Separation Science

A three‐dimensional graphene was synthesized through a hydrothermal reaction of graphene oxide with phytic acid. The microstructure and morphology of the phytic acid induced three‐dimensional graphene were investigated by nitrogen adsorption–desorption isotherms, scanning electron microscopy, and transmission electron microscopy. With a large surface area and three‐dimensional structure, the graphene was used as the solid‐phase extraction...

Hansen solubility parameters for assay method optimization of simvastatin, ramipril, atenolol, hydrochlorothiazide and aspirin in human plasma using liquid chromatography with tandem mass spectrometry

Published: Aug 14, 2017

Author(s): Murali Mohan Reddy Devalapalli, Hanumanth Srikanth Cheruvu, Thejaswi Yertha, Vijaya Bhaskar Veeravalli, Sunitha Sampathi, Savithri Shivakumar

Journal: Journal of Separation Science

A simple, specific, sensitive, validated method was developed using liquid chromatography with tandem mass spectrometry with electrospray ionization of human plasma for the simultaneous estimation of drugs (simvastatin, ramipril, atenolol, hydrochlorothiazide, and aspirin) of PolycapTM capsule used in cardiovascular therapy. The interaction of these actives including internal standards between the stationary and mobile phase were...

A new sample preparation and separation combination for the precise, accurate, and simultaneous determination of uracil and dihydrouracil in human plasma by reversed‐phase HPLC

Published: Aug 14, 2017

Author(s): Wen Pan, Yuandong Li, Yan Feng, Fan Yang, Haizhou Liu

Journal: Journal of Separation Science

We have developed an efficient procedure and detection method using reversed‐phase high‐performance liquid chromatography for the simultaneous measurement of uracil and dihydrouracil in human plasma. The procedure, including chromatographic conditions and sample preparation, was optimized and validated. Optimization of the sample preparation included deproteinization, extraction, and cleanup. A new sample preparation method which resulted...

Identification of metabolites in human and rat urine after oral administration of Xiao‐Qing‐Long‐Tang granule using ultra high performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry

Published: Aug 14, 2017

Author(s): Lei Zhou, Qiang Zhang, Wen Qi, Shuai Yan, Jialin Qu, Toshiaki Makino, Dan Yuan

Journal: Journal of Separation Science

Xiao‐Qing‐Long‐Tang is a traditional Chinese formula used for the treatment of cold syndrome, bronchitis, and nasal allergies for thousands of years. However, the in vivo integrated metabolism of its multiple components and the active chemical constituents of Xiao‐Qing‐Long‐Tang remain unknown. In this study, a method using ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry was...