EarlyView Articles

Skip to Navigation

The very latest articles from the scientific literature
on Wiley Online Library...


You can keep up-to date on these EarlyView articles, which focus on spectroscopy
and separation science:


Please check back soon.

Wiley Online Library

Earlyview RSS Feed

rss

Filter by Journal

Browse By Journal

Elucidation of the absolute configuration of rhizopine by chiral supercritical fluid chromatography and vibrational circular dichroism

Published: Jul 7, 2015

Author(s): Alain Krief, Melissa Dunkle, Masoud Bahar, Patrick Bultinck, Wouter Herrebout, Pat Sandra

Journal: Journal of Separation Science

The absolute configuration of rhizopine, an opine‐like natural product present in nitrogen‐fixing nodules of alfalfa infected by rhizobia, is elucidated using a combination of state‐of‐the‐art analytical and semi‐preparative supercritical fluid chromatography and vibrational circular dichroism spectroscopy. A synthetic peracetylated racemate was fractionated into its enantiomers and subjected to absolute configuration analysis revealing...

Read More thumbnail image: Elucidation of the absolute configuration of rhizopine by chiral supercritical fluid chromatography and vibrational circular dichroism

Structure refinement for La 1.85 Sr 0.15 Cu 1 − x Ni x O 4 ( x  = 0–0.19) transition‐metal oxides

Published: Jul 7, 2015

Author(s): R. Minikayev, V. Bezusyy, A. Malinowski

Journal: X-Ray Spectrometry

Polycrystalline La1.85Sr0.15Cu1 − xNixO4 (0 ≤ x ≤ 0.19) solid solution with the K2NiF4 structure (S.G. I4/mmm) is a subject of structural investigation. Rietveld refinements performed for 16 samples provide a smooth linear or almost linear variation of lattice parameters, La(Sr) atomic coordinate and bond distances with Ni content. The zO(2) oxygen coordinate tends to be constant in the studied range. The present results are compared with previous data and...

Read More thumbnail image: Structure refinement for La 1_85 Sr 0_15 Cu 1x Ni x O 4  x 00_19 transitionmetal oxides

Adsorption of cinnabarinic acid from culture fluid with magnetic microbeads

Published: Jul 6, 2015

Author(s): Aslı Göçenoğlu Sarıkaya, Bilgen Osman, Ali Kara, Nurdan Pazarlioglu, Necati Beşirli

Journal: Biomedical Chromatography

Abstract In this study, antimicrobial pigment cinnabarinic acid (CA) was produced from Pycnoporus cinnabarinus in laboratory‐scale batch cultures. Magnetic poly(ethylene glycol dimethacrylate‐N‐methacryloyl‐l‐tryptophan methyl ester) [m‐poly(EGDMA‐MATrp)] beads (average diameter = 53–103 µm) were synthesized by copolymerizing of N‐methacryloyl‐l‐tryptophan methyl ester (MATrp) with ethylene glycol dimethacrylate (EGDMA) in the presence of magnetite (Fe3O4) and used...

Read More thumbnail image: Adsorption of cinnabarinic acid from culture fluid with magnetic microbeads

Simultaneous determination of four secoiridoid and iridoid glycosides in rat plasma by ultra performance liquid chromatography–tandem mass spectrometry and its application to a comparative pharmacokinetic study

Published: Jul 6, 2015

Author(s): Yun Wu, Yu Ai, Fenrong Wang, Wen Ma, Qiaoxia Bian, David Y.‐W. Lee, Ronghua Dai

Journal: Biomedical Chromatography

Abstract A simple, reliable and rapid ultra‐performance liquid chromatography–tandem mass spectrometry method was developed and validated for the simultaneous quantification of four secoiridoid (gentiopicroside, swertiamarin, sweroside) and iridoid glycosides (loganic acid), the bio‐active ingredients in rat plasma. After liquid–liquid extraction, chromatographic separation was accomplished on a Shim‐pack XR‐ODS column with a mobile...

Read More thumbnail image: Simultaneous determination of four secoiridoid and iridoid glycosides in rat plasma by ultra performance liquid chromatographytandem mass spectrometry and its application to a comparative pharmacokinetic study

In vitro protein profiles in the early and late stages of Douglas‐fir xylogenesis

Published: Jul 6, 2015

Author(s): Jowita A. Dziedzic, Armando G. McDonald

Journal: ELECTROPHORESIS

The process of wood formation is of great interest to control and manipulate wood quality for economically important gymnosperms. A Douglas‐fir tissue culture system was developed that could be induced to differentiate into tracheary elements (fibers) making it possible to monitor xylogenesis in vitro by a proteomics approach. Two proteomes were analyzed and compared, one from an early and one from a late stage of the fiber...

Read More thumbnail image: In vitro protein profiles in the early and late stages of Douglasfir xylogenesis

Microchip‐based forensic short tandem repeat genotyping

Published: Jul 6, 2015

Author(s): Yong Tae Kim, Hyun Young Heo, Shin Hye Oh, Seung Hwan Lee, Do Hyun Kim, Tae Seok Seo

Journal: ELECTROPHORESIS

Micro total analysis system (μTAS) or lab‐on‐a‐chip (LOC) technology has advanced over decades, and the high performance for chemical and biological analysis has been well demonstrated with advantages of low sample consumption, rapid analysis time, high‐throughput screening, and portability. In particular, μTAS or LOC based genetic applications have been extensively explored, and the short tandem repeat (STR) typing on a chip has garnered...

Read More thumbnail image: Microchipbased forensic short tandem repeat genotyping

Solid phase extraction methodology for UPLC‐MS based metabolic profiling of urine samples

Published: Jul 6, 2015

Author(s): Filippos Michopoulos, Helen Gika, Dimitrios Palachanis, Georgios Theodoridis, Ian D. Wilson

Journal: ELECTROPHORESIS

In general, when performing untargeted metabolic phenotyping (metabolomics/metabonomics) studies on biological samples for example urine or food, sample preparation should be kept to a minimum. However, there are circumstances when desalting, preconcentration, or the fractionation of samples into polar and nonpolar metabolites is of value for enabling the subsequent analysis. Because of its simplicity and ease of automation SPE is well...

Read More thumbnail image: Solid phase extraction methodology for UPLCMS based metabolic profiling of urine samples

Stability of energy metabolites—An often overlooked issue in metabolomics studies: A review

Published: Jul 6, 2015

Author(s): Andres Gil, David Siegel, Hjalmar Permentier, Dirk‐Jan Reijngoud, Frank Dekker, Rainer Bischoff

Journal: ELECTROPHORESIS

Recent advances in analytical chemistry have set the stage for metabolite profiling to help understand complex molecular processes in physiology. Despite ongoing efforts, there are concerns regarding metabolomics workflows, since it has been shown that internal (enzyme activity, blood contamination, and the dynamic nature of metabolite concentrations) as well as external factors (storage, handling, and analysis method) may affect the...

Read More thumbnail image: Stability of energy metabolitesAn often overlooked issue in metabolomics studies A review

Signal enhancement in ultraflat electrochemical DNA biosensors

Published: Jul 6, 2015

Author(s): Wilmer A. Pardo, Mònica Mir, Josep Samitier

Journal: ELECTROPHORESIS

The ability of holding back the undesired molecules, but at the same time to provide the right distribution and orientation of the bioreceptors, are critical targets to reach an efficient hybridization and enhanced detection in electrochemical DNA biosensors. The main actors responsible of these key functions are the substrate of the sensor and the interface auto‐assembled on it. In this paper we present the annealing as a method to...

Read More thumbnail image: Signal enhancement in ultraflat electrochemical DNA biosensors

Measurement of vascular water transport in human subjects using time‐resolved pulsed arterial spin labelling

Published: Jul 5, 2015

Author(s): Adnan Bibic, Linda Knutsson, Anders Schmidt, Erik Henningsson, Sven Månsson, Kasim Abul‐Kasim, Jonas Åkeson, Matthias Gunther, Freddy Ståhlberg, Ronnie Wirestam

Journal: NMR in Biomedicine

Most approaches to arterial spin labelling (ASL) data analysis aim to provide a quantitative measure of the cerebral blood flow (CBF). This study, however, focuses on the measurement of the transfer time of blood water through the capillaries to the parenchyma (referred to as the capillary transfer time, CTT) as an alternative parameter to characterise the haemodynamics of the system. The method employed is based on a non‐compartmental...

Read More thumbnail image: Measurement of vascular water transport in human subjects using timeresolved pulsed arterial spin labelling

Viability and MR detectability of iron labeled mesenchymal stem cells used for endoscopic injection into the porcine urethral sphincter

Published: Jul 5, 2015

Author(s): Susanne Will, Petros Martirosian, Frank Eibofner, Fritz Schick, Rüdiger Bantleon, Martin Vaegler, Gerd Grözinger, Claus D. Claussen, Ulrich Kramer, Jörg Schmehl

Journal: NMR in Biomedicine

Direct stem cell therapies for functionally impaired tissue require a sufficient number of cells in the target region and a method for verifying the fate of the cells in the subsequent time course. In vivo MRI of iron labeled mesenchymal stem cells has been suggested to comply with these requirements. The study was conducted to evaluate proliferation, migration, differentiation and adhesion effects as well as the obtained iron load of an iron...

Read More thumbnail image: Viability and MR detectability of iron labeled mesenchymal stem cells used for endoscopic injection into the porcine urethral sphincter

Thermal expansion of polycrystalline gallium nitride: an X‐ray diffraction study

Published: Jul 3, 2015

Author(s): R. Minikayev, W. Paszkowicz, P. Piszora, M. Knapp, C. Bähtz, S. Podsiadło

Journal: X-Ray Spectrometry

Gallium nitride is studied at ambient and nonambient temperatures by powder X‐ray diffraction followed by Rietveld refinement of the structure. The structure is reported for the ambient temperature on the basis of laboratory data. The diffraction data collected using a synchrotron beam serve for derivation of the lattice parameter and thermal expansion dependencies on temperature. The variation of unit‐cell size on temperature was studied...

Read More thumbnail image: Thermal expansion of polycrystalline gallium nitride an Xray diffraction study

Prospects of X‐ray photoemission electron microscopy at the first beamline of the Polish synchrotron facility ‘Solaris’

Published: Jul 3, 2015

Author(s): M. Ślęzak, T. Giela, D. Wilgocka‐Ślęzak, N. Spiridis, T. Ślęzak, M. Zając, A. Kozioł‐Rachwał, R. P. Socha, M. Stankiewicz, P. Warnicke, N. Pilet, J. Raabe, C. Quitmann, J. Korecki

Journal: X-Ray Spectrometry

The synchrotron radiation facility ‘Solaris’ is currently being built in Krakow, Poland. The first experimental beamline at Solaris will use bending magnet radiation and two exchangeable end stations: a spectroscopic X‐ray photoemission electron microscope and a soft X‐ray absorption spectroscopy chamber. We present the beamline specifications and exemplary results obtained with our end station microscope, which (in statu nascendi of...

Read More thumbnail image: Prospects of Xray photoemission electron microscopy at the first beamline of the Polish synchrotron facility Solaris

Assessement of the pharmacokinetics, tissue distribution and excretion studies of a novel antiplatelet agent S007‐867, following administration to rats

Published: Jul 3, 2015

Author(s): Hardik Chandasana, Yashpal S. Chhonker, Tulsankar Sachin Laxman, Yarra Durga Prasad, Anil Kumar K. S., Dinesh K. Dikshit, Rabi S. Bhatta

Journal: Drug Testing and Analysis

S007‐867 is a promising novel antiplatelet agent with better efficacy and lesser bleeding risk than existing agents. The present study investigated the absorption, tissue distribution, and excretion of S007‐867 in rat model for further advancement of the molecule. A simple and robust ultra fast liquid chromatography‐tandem mass spectrometry (UFLC‐MS/MS) bioanalytical method was used to determine S007‐867 in various matrices. Following...

Read More thumbnail image: Assessement of the pharmacokinetics tissue distribution and excretion studies of a novel antiplatelet agent S007867 following administration to rats

Analytical Quality by Design in pharmaceutical quality assurance: Development of a capillary electrophoresis method for the analysis of zolmitriptan and its impurities

Published: Jul 3, 2015

Author(s): Serena Orlandini, Benedetta Pasquini, Claudia Caprini, Massimo Del Bubba, Sergio Pinzauti, Sandra Furlanetto

Journal: ELECTROPHORESIS

A fast and selective CE method for the determination of zolmitriptan (ZOL) and its five potential impurities has been developed applying the analytical Quality by Design principles. Voltage, temperature, buffer concentration, and pH were investigated as critical process parameters that can influence the critical quality attributes, represented by critical resolution values between peak pairs, analysis time, and peak efficiency of...

Read More thumbnail image: Analytical Quality by Design in pharmaceutical quality assurance Development of a capillary electrophoresis method for the analysis of zolmitriptan and its impurities

Characterization of 2D colloid aggregations created by optically induced electrohydrodynamics

Published: Jul 3, 2015

Author(s): Andrew H. Work, Stuart J. Williams

Journal: ELECTROPHORESIS

Rapid electrokinetic patterning (REP) is a technique for creating self‐assembled monolayers (SAMs) of spherical particles in a liquid medium, and dynamically controlling them though the simultaneous application of an electric field and optically induced temperature gradients. Previous work has investigated and characterized REP axisymmetric aggregations generated from a focus laser within a uniform electric field; work herein...

Read More thumbnail image: Characterization of 2D colloid aggregations created by optically induced electrohydrodynamics

Capillary electrophoresis coupled to contactless conductivity detection for the analysis of S‐nitrosothiols decomposition and reactivity

Published: Jul 3, 2015

Author(s): Abdulghani Ismail, Fanny d'Orlyé, Sophie Griveau, Fethi Bedioui, Anne Varenne, José Alberto Fracassi da Silva

Journal: ELECTROPHORESIS

S‐Nitrosothiols (RSNO) are composed of a NO group bound to the sulfhydryl group of a peptide or protein. RSNO are very important biological molecules, since they have many effects on human health. RSNO are easily naturally decomposed by metal ions, light, and heat, with different kinetics. They can furthermore undergo transnitrosation (NO moieties exchange), which is a crucial point in physiological conditions since the concentration...

Read More thumbnail image: Capillary electrophoresis coupled to contactless conductivity detection for the analysis of Snitrosothiols decomposition and reactivity

Microparticles released from Mycobacterium tuberculosis ‐infected human macrophages contain increased levels of the type I interferon inducible proteins including ISG15

Published: Jul 3, 2015

Author(s): Nathan J. Hare, Brian Chan, Edwina Chan, Kimberley L. Kaufman, Warwick J. Britton, Bernadette M. Saunders

Journal: PROTEOMICS

Microparticles (MPs) are small membranous particles (100–1000 nm) released under normal steady‐state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these MPs induced a proinflammatory response from uninfected macrophages in vitro and in vivo following their transfer into uninfected mice....

Read More thumbnail image: Microparticles released from Mycobacterium tuberculosis infected human macrophages contain increased levels of the type I interferon inducible proteins including ISG15

Proteomic profiling of human plasma identifies apolipoprotein E as being associated with smoking and a marker for squamous metaplasia of the lung

Published: Jul 3, 2015

Author(s): Shawn J. Rice, Xin Liu, Bruce Miller, Monika Joshi, Junjia Zhu, Carla Caruso, Chris Gilbert, Jennifer Toth, Michael Reed, Negar Rassaei, Arun Das, Amit Barochia, Karam El‐Bayoumy, Chandra P. Belani

Journal: PROTEOMICS

Biomarkers to identify subjects at high‐risk for developing lung cancer will revolutionize the disease outlook. Most biomarker studies have focused on patients already diagnosed with lung cancer and in most cases the disease is often advanced and incurable. The objective of this study was to use proteomics to identify a plasma biomarker for early detection of lung lesions that may subsequently be the harbinger for cancer. Plasma samples...

Read More thumbnail image: Proteomic profiling of human plasma identifies apolipoprotein E as being associated with smoking and a marker for squamous metaplasia of the lung

Enhancing bottom‐up and top‐down proteomic measurements with ion mobility separations

Published: Jul 3, 2015

Author(s): Erin Shammel Baker, Kristin E. Burnum‐Johnson, Yehia M. Ibrahim, Daniel J. Orton, Matthew E. Monroe, Ryan T. Kelly, Ronald J. Moore, Xing Zhang, Roger Théberge, Catherine E. Costello, Richard D. Smith

Journal: PROTEOMICS

Proteomic measurements with greater throughput, sensitivity, and structural information are essential for improving both in‐depth characterization of complex mixtures and targeted studies. While LC separation coupled with MS (LC–MS) measurements have provided information on thousands of proteins in different sample types, the introduction of a separation stage that provides further component resolution and rapid structural information has...

Read More thumbnail image: Enhancing bottomup and topdown proteomic measurements with ion mobility separations
Page:   1 2 3 4 5 6 Next

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2015 John Wiley & Sons, Inc. All Rights Reserved