EarlyView Articles
The very latest articles from the scientific literature
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Effect of the sol–gel route on the textural characteristics of silica imprinted with Rhodamine B
Published: Mar 7, 2014
Author(s): Cícero de Coelho Escobar, João Henrique Zimnoch dos Santos
Journal: Journal of Separation Science
A series of silica xerogels that support Rhodamine B as a template were synthesized using distinct sol–gel routes, namely, acid‐catalyzed routes, a base‐catalyzed route, acid‐catalyzed with base‐catalyzed (two steps) hydrolytic routes, and a FeCl3‐catalyzed nonhydrolytic route. The extraction methods (thermal, Soxhlet, water washing, and ultrasound) were also evaluated. The resulting xerogels were characterized through porosimetry using nitrogen...
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Synchronized separation of seven medications representing most commonly prescribed antihypertensive classes by using reversed‐phase liquid chromatography: Application for analysis in their combined formulations
Published: Mar 7, 2014
Author(s): Walid M. Ebeid, Ehab F. Elkady, Asmaa A. El‐Zaher, Ramzia I. El‐Bagary, Gabor Patonay
Journal: Journal of Separation Science
A reversed‐phase high‐performance liquid chromatography method was developed for the simultaneous determination of the diuretic, hydrochlorothiazide, along with six drugs representing the most commonly prescribed antihypertensive pharmacological classes such as atenolol, a selective β1 blocker, amlodipine besylate, a calcium channel blocker, moexipril hydrochloride, an angiotensin‐converting‐enzyme inhibitor, valsartan and candesartan...
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Analysis of estrogens and estrogen mimics in edible matrices—A review
Published: Mar 7, 2014
Author(s): Hana Adamusova, Zuzana Bosakova, Pavel Coufal, Vera Pacakova
Journal: Journal of Separation Science
This review provides a brief survey of the biological effects of selected endocrine‐disrupting compounds that are formed after internal exposure of organisms. Further, the present analytical methods available for the determination of these compounds in foodstuffs are critically evaluated. The attention is primarily devoted to the methods for sample pretreatment, which are the main source of errors and are usually the most time‐consuming...
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Comparison of various techniques for the extraction and determination of antioxidants in plants
Published: Mar 7, 2014
Author(s): Petra Bajerová, Martin Adam, Tomáš Bajer, Karel Ventura
Journal: Journal of Separation Science
The following extraction techniques have been used for extracting antioxidants (apigenin, coumarin, esculetin, umbelliferone, bergapten, quercetin, rutin, scopoletin and xanthotoxin) from plant material: supercritical fluid extraction, pressurized liquid extraction, extraction by means of Soxhlet apparatus, ultrasonic extraction in ultrasonic bath, and by means of ultrasonic probe. The analytical method based on HPLC−UV detection for the...
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Simultaneous determination of erlotinib and metabolites in human urine using capillary electrophoresis
Published: Mar 7, 2014
Author(s): Juana Rodríguez, Gregorio Castañeda, Lorena Muñoz, Diana Navarro, Jose C. Villa
Journal: ELECTROPHORESIS
The purpose of this study was to develop a simple and sensitive CE‐UV method to quantify erlotinib and metabolites in urine. Following liquid–liquid extraction, erlotinib, and metabolites were separated with a BGE whose composition was phosphate buffer (pH 2.5, 65 mM) with 0.5% Tween 20. The applied voltage was 22 kV, capillary temperature 25°C and the sample injection was performed in the hydrodynamic mode. All the analyses were carried...
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Self‐organizing maps: A versatile tool for the automatic analysis of untargeted imaging datasets
Published: Mar 7, 2014
Author(s): Pietro Franceschi, Ron Wehrens
Journal: PROTEOMICS
MS‐based imaging approaches allow for location‐specific identification of chemical components in biological samples, opening up possibilities of much more detailed understanding of biological processes and mechanisms. Data analysis, however, is challenging, mainly because of the sheer size of such datasets. This article presents a novel approach based on self‐organizing maps, extending previous work in order to be able to handle the large...
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Compound heterozygotes and beta‐thalassemia: Top‐down mass spectrometry for detection of hemoglobinopathies
Published: Mar 7, 2014
Author(s): Rebecca L. Edwards, Paul Griffiths, Josephine Bunch, Helen J. Cooper
Journal: PROTEOMICS
We have shown previously that liquid extraction surface analysis of dried blood spots coupled to high resolution top‐down MS may be applied for the diagnosis of hemoglobin (Hb) variants FS, FAS, FC, FAC, FAD in newborn samples. The objective of the current work was to determine whether the structural variant HbE, compound heterozygote variants FSC and FSD, and β‐thalassemia were amenable to diagnosis by this approach. Anonymized residual...
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Characterization of the designer benzodiazepine diclazepam and preliminary data on its metabolism and pharmacokinetics
Published: Mar 6, 2014
Author(s): Bjoern Moosmann, Philippe Bisel, Volker Auwärter
Journal: Drug Testing and Analysis
Designer benzodiazepines, first offered in online shops selling ‘research chemicals’ in 2012, provide an attractive alternative to prescription‐only benzodiazepines as they are readily available over the Internet at a low price. However, as data regarding pharmacokinetic parameters, metabolism, and detectability in biological fluids are limited, they present a challenge for forensic laboratories. Most recently, diclazepam (other names:...
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Determination of allergenic hydroperoxides in essential oils using gas chromatography with electron ionization mass spectrometry
Published: Mar 6, 2014
Author(s): Johanna Rudbäck, Ahmed Ramzy, Ann‐Therese Karlberg, Ulrika Nilsson
Journal: Journal of Separation Science
Fragrance monoterpenes are widely used commercially due to their pleasant scent. In previous studies, we have shown that air‐exposed monoterpenes form hydroperoxides that are strong skin sensitizers. Methods for detection and quantification of the hydroperoxides in essential oils and scented products are thus desirable. Due to thermolability and low UV absorbance, this is a complicated task. We have recently developed a sensitive...
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Characterization of metabolites in infiltrating gliomas using ex vivo 1 H high‐resolution magic angle spinning spectroscopy
Published: Mar 5, 2014
Author(s): Adam Elkhaled, Llewellyn Jalbert, Alexandra Constantin, Hikari A. I. Yoshihara, Joanna J. Phillips, Annette M. Molinaro, Susan M. Chang, Sarah J. Nelson
Journal: NMR in Biomedicine
Gliomas are routinely graded according to histopathological criteria established by the World Health Organization. Although this classification can be used to understand some of the variance in the clinical outcome of patients, there is still substantial heterogeneity within and between lesions of the same grade. This study evaluated image‐guided tissue samples acquired from a large cohort of patients presenting with either new or...
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Analysis of ibuprofen enantiomers in rat plasma by liquid chromatography with tandem mass spectrometry
Published: Mar 5, 2014
Author(s): Juciane Lauren Cavalcanti Cardoso, Vera Lucia Lanchote, Maria Paula Marques Pereira, Natália Valadares de Moraes, José Salvador Lepera
Journal: Journal of Separation Science
A sensitive and selective method for the analysis of ibuprofen enantiomers by LC–MS/MS was developed and validated for the purpose of application in pharmacokinetic studies in small experimental animals. Aliquots of 200 μL plasma were submitted to liquid–liquid extraction with hexane/diisopropylether (50:50 v/v) in acid pH. Separation was accomplished in a Chirex® 3005 (250 × 4.6 mm, 5 μm) column at 25°C with a mobile phase that consisted...
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Imaging mass spectrometry to discriminate breast from pancreatic cancer metastasis in formalin‐fixed paraffin‐embedded tissues
Published: Mar 5, 2014
Author(s): Rita Casadonte, Mark Kriegsmann, Friederike Zweynert, Katrin Friedrich, Gustavo Bretton, Mike Otto, Sören‐Oliver Deininger, Rainer Paape, Eckhard Belau, Detlev Suckau, Daniela Aust, Christian Pilarsky, Jörg Kriegsmann
Journal: PROTEOMICS
Diagnosis of the origin of metastasis is mandatory for adequate therapy. In the past, classification of tumors was based on histology (morphological expression of a complex protein pattern), while supportive immunohistochemical investigation relied only on few “tumor specific” proteins. At present, histopathological diagnosis is based on clinical information, morphology, immunohistochemistry, and may include molecular methods. This...
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The utilization of fluorescence to identify the components of lipofuscin by imaging mass spectrometry
Published: Mar 5, 2014
Author(s): Zsolt Ablonczy, Noah Smith, David M. Anderson, Angus C. Grey, Jeffrey Spraggins, Yiannis Koutalos, Kevin L. Schey, Rosalie K. Crouch
Journal: PROTEOMICS
Lipofuscin, an aging marker in the retinal pigment epithelium (RPE) associated with the development of age‐related macular degeneration, is primarily characterized by its fluorescence. The most abundant component of RPE lipofuscin is N‐retinylidene‐N‐retinylethanolamine (A2E) but its exact composition is not known due to the complexity of the RPE extract. In this study, we utilized MALDI imaging to find potential molecules responsible for lipofuscin...
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Miniaturized molecularly imprinted polymer extraction method for the gas chromatographic analysis of flavonoids
Published: Mar 5, 2014
Author(s): Yada Nolvachai, Chadin Kulsing, Reinhard I. Boysen, Milton T.W. Hearn, Philip J. Marriott
Journal: Journal of Separation Science
In this study, the use of monolithic molecularly imprinted polymers in a micropipette tip format allowing the simple and fast extraction of flavonoids from standard solutions and a black tea sample is demonstrated. The imprinted polymer employed quercetin, methacrylic acid or 4‐vinylpyridine, and ethylene glycol dimethacrylate as template, functional monomer, and cross‐linker, respectively. Surface morphologies of the quercetin‐imprinted...
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The rise of new psychoactive substance use in Australia
Published: Mar 5, 2014
Author(s): Lucy Burns, Amanda Roxburgh, Allison Matthews, Raimondo Bruno, Simon Lenton, Joe Van Buskirk
Journal: Drug Testing and Analysis
New Psychoactive Substances are now a feature of Australia's recreational drug market. Little is known, however, about the prevalence of use, the characteristics of people who use them and the relationship between the NPS and ecstasy markets. This study examined the prevalence and correlates of NPS use amongst a group of regular ecstasy users in Australia. Participants were recruited if they had used ecstasy at least six times in the...
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Spatial blockage of ionic current for electrophoretic translocation of DNA through a graphene nanopore
Published: Mar 5, 2014
Author(s): Wenping Lv, Shengju Liu, Xin Li, Ren'an Wu
Journal: ELECTROPHORESIS
Graphene nanopore has been promising the ultra‐high resolution for DNA sequencing due to the atomic thickness and excellent electronic properties of the graphene monolayer. The dynamical translocation phenomena and/or behaviors underneath the blocked ionic current, however, have not been well unveiled to date for the translocation of DNA electrophoretically through a graphene nanopore. In this report, the assessment on the sensitivity of...
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Sialic acid methylation refines capillary electrophoresis laser‐induced fluorescence analyses of immunoglobulin G N ‐glycans of ovarian cancer patients
Published: Mar 5, 2014
Author(s): Christian Schwedler, Matthias Kaup, Dominique Petzold, Berthold Hoppe, Elena Iona Braicu, Jalid Sehouli, Marc Ehlers, Markus Berger, Rudolf Tauber, Véronique Blanchard
Journal: ELECTROPHORESIS
Alterations in IgG N‐glycosylation coincide with the development of a number of diseases including cancer and could potentially be used as diagnostic markers. CE‐LIF of 8‐aminopyrene‐1,3,6‐trisulfonic acid labeled N‐glycans is a well‐established rapid method to characterize IgG N‐glycans that needs only low amounts of starting material. However, sialylated N‐glycans have short migration times due to their negative charge. As a result, some of them are not well...
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Growth inhibition and differences in protein profiles in azadirachtin‐treated Drosophila melanogaster larvae
Published: Mar 5, 2014
Author(s): Hao Wang, Duo Lai, Mei Yuan, Hanhong Xu
Journal: ELECTROPHORESIS
Azadirachtin A is a very effective biopesticide widely used in insect pest control. It has strong antifeeding and growth inhibitory activity against most insects, however, its mode of action is still unclear. Proteomic experiments using 2DE indicate significant effects of Azadirachtin A on the amount of proteins related to growth inhibition in Drosophila melanogaster larvae. Twenty‐one spots with different intensity in azadirachtin‐treated larvae...
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Methodological aspects of an off‐line combination of preparative isotachophoresis and high‐performance liquid chromatography with mass spectrometry in the analysis of biological matrices
Published: Mar 5, 2014
Author(s): Monika Kondeková, Andrea Staňová, Jozef Marák
Journal: ELECTROPHORESIS
This work reports on some methodological aspects of an off‐line combination of preparative ITP and HPLC with mass spectrometric detection (pITP‐HPLC‐MS) and its potential applications to the analysis of high molecular mass compounds present in complex biological matrices from the analytical chemistry perspective. Lysozyme served as the model analyte and human saliva as the complex biological matrix in this study. A mixture of five...
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The foodomics approach for the evaluation of protein bioaccessibility in processed meat upon in vitro digestion
Published: Mar 5, 2014
Author(s): Alessandra Bordoni, Luca Laghi, Elena Babini, Mattia Di Nunzio, Gianfranco Picone, Alessandra Ciampa, Veronica Valli, Francesca Danesi, Francesco Capozzi
Journal: ELECTROPHORESIS
The present work describes a foodomics protocol coupling an in vitro static simulation of digestion to a combination of omics techniques, to grant an overview of the protein digestibility of a meat‐based food, namely Bresaola. The proteolytic activity mediated by the digestive enzymes is evaluated through Bradford and SDS‐PAGE assays, combined to NMR relaxometry and spectroscopy, to obtain information ranging from the microscopic to the...
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