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Synthesis of a metal–organic framework confined in periodic mesoporous silica with enhanced hydrostability as a novel fiber coating for solid‐phase microextraction

Published: Mar 3, 2015

Author(s): Mir Mahdi Abolghasemi, Vahid Yousefi, Marzieh Piryaei

Journal: Journal of Separation Science

A metal–organic framework/periodic mesoporous silica (MOF‐5@SBA‐15) hybrid material has been prepared by using SBA‐15 as a matrix. The prepared MOF‐5@SBA‐15 hybrid material was then deposited on a stainless‐steel wire to obtain the fiber for the solid‐phase microextraction of phenolic compounds. Modifications in the metal–organic framework structure have proven to improve the extraction performance of MOF/SBA‐15 hybrid materials, compared...

Read More thumbnail image: Synthesis of a metalorganic framework confined in periodic mesoporous silica with enhanced hydrostability as a novel fiber coating for solidphase microextraction

Universal method for the determination of nonionic surfactant content in the presence of protein

Published: Mar 3, 2015

Author(s): Ziping Wei, Susanna Bilbulian, Jingning Li, Ratnesh Pandey, Ellen O'Connor, Jose Casas‐Finet, Patricia W. Cash

Journal: Journal of Separation Science

A new analytical method has been developed for the quantitative determination of ethylene glycol‐containing nonionic surfactants, such as polyethylene glycol 8000, polysorbate 80, and Pluronic F‐68. These surfactants are commonly used in pharmaceutical protein preparations, thus, testing in the presence of protein is required. This method is based on the capillary gas chromatographic analysis of ethylene glycol diacetate formed by...

Read More thumbnail image: Universal method for the determination of nonionic surfactant content in the presence of protein

Compartment resolved reference proteome map from highly purified naïve, activated, effector, and memory CD8 + murine immune cells

Published: Mar 3, 2015

Author(s): Damien Zanker, Wolfgang Otto, Weisan Chen, Martin Bergen, Janina M. Tomm

Journal: PROTEOMICS

Differentiation of CD8+ T lymphocytes into effector and memory cells is key for an adequate immune response and relies on complex interplay of pathways that convey signals from the cell surface to the nucleus. In this study, we investigated the proteome of four cytotoxic T‐cell subtypes; naïve, recently activated effector, effector, and memory cells. Cells were fractionated into membrane, cytosol, soluble nuclear, chromatin‐bound, and...

Read More thumbnail image: Compartment resolved reference proteome map from highly purified naive activated effector and memory CD8  murine immune cells

Scop3D: Three‐dimensional visualization of sequence conservation

Published: Mar 3, 2015

Author(s): Tessa Vermeire, Stijn Vermaere, Bert Schepens, Xavier Saelens, Steven Gucht, Lennart Martens, Elien Vandermarliere

Journal: PROTEOMICS

The integration of a protein's structure with its known sequence variation provides insight on how that protein evolves, for instance in terms of (changing) function or immunogenicity. Yet, collating the corresponding sequence variants into a multiple sequence alignment, calculating each position's conservation, and mapping this information back onto a relevant structure is not straightforward. We therefore built the Sequence Conservation...

Read More thumbnail image: Scop3D Threedimensional visualization of sequence conservation

Modern utilization of an accurate method for detecting essential elements in whole blood using low energy photons

Published: Mar 3, 2015

Author(s): M. E. Medhat, W. Shan, M. Kurudirek

Journal: X-Ray Spectrometry

The objective of the proposed work was to measure concentration of six essential elements, Ca, Cu, Fe, Mn, Sr, and Zn directly in whole blood. The adopted method is based on the theory of attenuation of low energy photons through the whole blood sample. Photons are completely absorbed at energy region approximately (25–100 keV), of K‐absorption edge for especially low‐Z elements and at L‐absorption edge especially for high‐Z elements....

Read More thumbnail image: Modern utilization of an accurate method for detecting essential elements in whole blood using low energy photons

Synthesis of carbon nanotube/layered double hydroxide nanocomposite as a novel fiber coating for the headspace solid‐phase microextraction of phenols from water samples

Published: Mar 3, 2015

Author(s): Mir Mahdi Abolghasemi, Vahid Yousefi, Marzieh Piryaei

Journal: Journal of Separation Science

In this research, a carbon nanotube/layered double hydroxide nanocomposite was synthesized by an in situ growth route by electrostatic force. The prepared carbon nanotube/layered double hydroxide nanocomposite was successfully prepared and deposited on a stainless‐steel wire for the fabrication of the solid‐phase microextraction fiber. The fiber was evaluated for the extraction of phenolic compounds from water samples. Analytical merits...

Read More thumbnail image: Synthesis of carbon nanotubelayered double hydroxide nanocomposite as a novel fiber coating for the headspace solidphase microextraction of phenols from water samples

Determination of sparstolonin B by ultra‐high performance liquid chromatography coupled with triple quadrupole mass spectrometry: application to pharmacokinetic study of sparstolonin B in rat plasma

Published: Mar 2, 2015

Author(s): Nuo‐Shu Zou, Qiao‐Li Liang, Ping Li, Jie Liu, Xiao Liu, An Kang, Hai‐Shan Deng

Journal: Biomedical Chromatography

Abstract Sparstolonin B (SsnB), a spontaneous isocoumarin compound isolated from the tuber of Scirpus yagara Ohwi. (Cyperaceae), possesses potent anti‐inflammatory and antitumor activity. In the present study, a rapid and simple UHPLC/MS/MS method for determination of SsnB in rat plasma was developed and validated. Plasma samples were pretreated by liquid–liquid extraction with ethyl acetate containing rhein as an internal standard and...

Read More thumbnail image: Determination of sparstolonin B by ultrahigh performance liquid chromatography coupled with triple quadrupole mass spectrometry application to pharmacokinetic study of sparstolonin B in rat plasma

Preparation of molecularly imprinted polymers based on magnetic nanoparticles for the selective extraction of protocatechuic acid from plant extracts

Published: Mar 2, 2015

Author(s): Xiaoyu Xie, Fen Wei, Liang Chen, Sicen Wang

Journal: Journal of Separation Science

In this study, highly selective core–shell molecularly imprinted polymers on the surface of magnetic nanoparticles were prepared using protocatechuic acid as the template molecule. The resulting magnetic molecularly imprinted polymers were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X‐ray diffraction, and vibrating sample magnetometry. The binding performances of the prepared materials were...

Read More thumbnail image: Preparation of molecularly imprinted polymers based on magnetic nanoparticles for the selective extraction of protocatechuic acid from plant extracts

Quantitative proteomics of Arabidopsis shoot microsomal proteins reveals a cross‐talk between excess zinc and iron deficiency

Published: Mar 2, 2015

Author(s): Sajad Majeed Zargar, Rie Kurata, Shoko Inaba, Akira Oikawa, Risa Fukui, Yoshiyuki Ogata, Ganesh Kumar Agrawal, Randeep Rakwal, Yoichiro Fukao

Journal: PROTEOMICS

Iron (Fe) deficiency significantly effects plant growth and development. Plant symptoms under excess zinc (Zn) resemble symptoms of Fe‐deficient plants. To understand cross‐talk between excess Zn and Fe deficiency, we investigated physiological parameters of Arabidopsis plants and applied iTRAQ‐OFFGEL quantitative proteomic approach to examine protein expression changes in microsomal fraction from Arabidopsis shoots under those...

Read More thumbnail image: Quantitative proteomics of Arabidopsis shoot microsomal proteins reveals a crosstalk between excess zinc and iron deficiency

Proteomic dissection of plant responses to various pathogens

Published: Mar 2, 2015

Author(s): Xianping Fang, Jianping Chen, Liangying Dai, Huasheng Ma, Hengmu Zhang, Jian Yang, Fang Wang, Chengqi Yan

Journal: PROTEOMICS

During their growth and development, plants are vulnerable to the effects of a variety of pathogens. Proteomics technology plays an important role in research studies of plant defense mechanisms by mining the expression changes of proteins in response to various biotic stresses. This review article provides a comprehensive overview of the latest developments in international proteomic research on plant biotic stress. It summarizes the...

Read More thumbnail image: Proteomic dissection of plant responses to various pathogens

Preparation of a hybrid monolithic stationary phase with allylsulfonate for the rapid and simultaneous separation of cations in capillary ion chromatography

Published: Mar 2, 2015

Author(s): Aster Rahayu, Lee Wah Lim, Toyohide Takeuchi

Journal: Journal of Separation Science

A hybrid monolithic column with sulfonate functionality was successfully prepared for the simultaneous separation of common inorganic cations in ion‐exchange chromatographic mode through a simple and easy single‐step preparation method. The strong cation‐exchange moieties were provided directly from allylsulfonate, which worked as an organic monomer in the single‐step reaction. Inorganic cations (Li+, Na+, K+, NH4+, Cs+, Rb+, Mg2+, Ca2+,...

Read More thumbnail image: Preparation of a hybrid monolithic stationary phase with allylsulfonate for the rapid and simultaneous separation of cations in capillary ion chromatography

An ultra high performance liquid chromatography with tandem mass spectrometry method for plasma and cerebrospinal fluid pharmacokinetics of rhein in patients with traumatic brain injury after administration of rhubarb decoction

Published: Mar 2, 2015

Author(s): Yang Wang, Rong Fan, Jiekun Luo, Tao Tang, Zhihua Xing, Zian Xia, Weijun Peng, Wenzhu Wang, Huiying Lv, Wei Huang, Yizeng Liang, Lunzhao Yi, Hongmei Lu, Xi Huang

Journal: Journal of Separation Science

Damage of blood–brain barrier is a common result of traumatic brain injury. This damage can open the blood–brain barrier and allow drug passage. An ultraperformance liquid chromatography with tandem mass spectrometry method was established to determine the concentration of rhein in the biofluids (plasma and cerebrospinal fluid) of patients with a compromised blood–brain barrier following traumatic brain injury after rhubarb...

Read More thumbnail image: An ultra high performance liquid chromatography with tandem mass spectrometry method for plasma and cerebrospinal fluid pharmacokinetics of rhein in patients with traumatic brain injury after administration of rhubarb decoction

Determination of the triterpene glycosides in sea cucumbers by liquid chromatography with evaporative light scattering and mass spectrometry detection

Published: Mar 2, 2015

Author(s): Jie Yang, Yuanhong Wang, Ran Zhang, Tifngfu Jiang, Zhihua Lv

Journal: Journal of Separation Science

Holothurian triterpene glycosides possess various kinds of biological activities, including antifungal, cytotoxic, hemolytic, cytostatic, and immunomodulatory effects. In this study, a rapid extraction method of triterpene glycosides from sea cucumbers using a small column of C18 solid phase was first developed. Furthermore, a novel high‐performance liquid chromatography method coupled with evaporative light scattering detection and...

Read More thumbnail image: Determination of the triterpene glycosides in sea cucumbers by liquid chromatography with evaporative light scattering and mass spectrometry detection

Determination of thermodynamic p K a values of pharmaceuticals from five different groups using capillary electrophoresis

Published: Mar 2, 2015

Author(s): Mirta Zrnčić, Sandra Babić, Dragana Mutavdžić Pavlović

Journal: Journal of Separation Science

A determination of the thermodynamic acid dissociation constants (pKa) of 22 frequently used pharmaceuticals using capillary electrophoresis in aqueous media is presented in this work. The investigated pharmaceuticals belong to different pharmacological groups: macrolides, fluoroquinolones, sulfonamides, β‐lactams, tetracyclines, and other miscellaneous pharmaceuticals. The electrophoretic mobilities of the investigated analytes were monitored in a...

Read More thumbnail image: Determination of thermodynamic p K a values of pharmaceuticals from five different groups using capillary electrophoresis

Probing the mechanism of the interaction between l ‐cysteine‐capped‐CdTe quantum dots and Hg 2+ using capillary electrophoresis with ensemble techniques

Published: Feb 27, 2015

Author(s): Laifang Xu, Junjie Hao, Tao Yi, Yinyin Xu, Xiaoying Niu, Cuiling Ren, Hongli Chen, Xingguo Chen

Journal: ELECTROPHORESIS

A good understanding of the mechanism of interaction between quantum dots (QDs) and heavy metal ions is essential for the design of more effective sensor systems. In this work, CE was introduced to explore how l‐cysteine‐capped‐CdTe QDs (l‐cys‐CdTe QDs) interacts with Hg2+. The change in electrophoretic mobility can synchronously reflect the change in the composition and property of QDs. The effects of the free and capping ligands on the system are...

Read More thumbnail image: Probing the mechanism of the interaction between l cysteinecappedCdTe quantum dots and Hg 2 using capillary electrophoresis with ensemble techniques

New vinylester‐based monoliths as a new stationary phase for capillary electrochromatography

Published: Feb 27, 2015

Author(s): Çiğdem Kip, Ali Tuncel

Journal: ELECTROPHORESIS

Vinyl ester‐based monoliths are proposed as a new group of stationary phase for CEC. The capillary monolithic columns were prepared by using two vinyl ester monomers, vinyl pivalate (VPV), and vinyl decanoate (VDC) by using ethylene dimethacrylate (EDMA) as the cross‐linking agent, and 2‐acrylamido‐2‐methylpropane sulfonic acid as the charge‐bearing monomer. The monoliths with different pore structures and permeabilities were obtained by...

Read More thumbnail image: New vinylesterbased monoliths as a new stationary phase for capillary electrochromatography

Study of immobilized metal affinity chromatography sorbents for the analysis of peptides by on‐line solid‐phase extraction capillary electrophoresis‐mass spectrometry

Published: Feb 27, 2015

Author(s): Lorena Ortiz‐Martin, Fernando Benavente, Silvia Medina‐Casanellas, Estela Giménez, Victoria Sanz‐Nebot

Journal: ELECTROPHORESIS

Several commercial immobilized metal affinity chromatography sorbents were evaluated in this study for the analysis of two small peptide fragments of the amyloid β‐protein (Aβ) (Aβ(1–15) and Aβ(10–20) peptides) by on‐line immobilized metal affinity SPE‐CE (IMA‐SPE‐CE). The performance of a nickel metal ion (Ni(II)) sorbent based on nitrilotriacetic acid as a chelating agent was significantly better than two copper metal ion (Cu(II))...

Read More thumbnail image: Study of immobilized metal affinity chromatography sorbents for the analysis of peptides by online solidphase extraction capillary electrophoresismass spectrometry

Simultaneous quantification of 25 active constituents in the total flavonoids extract from Herba Desmodii Styracifolii by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

Published: Feb 27, 2015

Author(s): Panpan Guo, Wenying Yan, Qingjie Han, Chunying Wang, Zijian Zhang

Journal: Journal of Separation Science

A sensitive and selective high‐performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry method has been developed and validated for the simultaneous determination of 25 active constituents, including 21 flavonoids and four phenolic acids in the total flavonoids extract from Herba Desmodii Styracifolii for the first time. Among the 25 compounds, seven compounds including caffeic acid, acacetin,...

Read More thumbnail image: Simultaneous quantification of 25 active constituents in the total flavonoids extract from Herba Desmodii Styracifolii by highperformance liquid chromatography with electrospray ionization tandem mass spectrometry

Preparation of 17β‐estradiol‐imprinted material by surface‐initiated atom transfer radical polymerization and its application

Published: Feb 27, 2015

Author(s): Yanru Gong, Yuling Niu, Xiaohan Gong, Meihua Ma, Xiaowei Ren, Weihua Zhu, Ruiming Luo, Bolin Gong

Journal: Journal of Separation Science

A novel 17β‐estradiol molecularly imprinted polymer was grafted onto the surface of initiator‐immobilized silica by surface‐initiated atom transfer radical polymerization. The resulting molecularly imprinted polymer was characterized by elemental analysis and thermogravimetric analysis. The binding property of molecularly imprinted polymer for 17β‐estradiol was also studied with both static and dynamic methods. The results showed that the...

Read More thumbnail image: Preparation of 17estradiolimprinted material by surfaceinitiated atom transfer radical polymerization and its application

Preparation of 4‐butylaniline‐bonded silica gel for the solid‐phase extraction of flavone glycosides

Published: Feb 27, 2015

Author(s): Ganghui Chu, Wensheng Cai, Xueguang Shao

Journal: Journal of Separation Science

To extract flavone glycosides efficiently, a new extraction material based on 4‐butylaniline‐bonded silica gel was prepared using a two‐step grafting method including a ring‐opening reaction and synchronous hydrolysis. Preparation of the silica‐based material was easily achieved under mild conditions, and the material was characterized by Fourier transform infrared spectroscopy, elemental analysis, and scanning electron microscopy. The...

Read More thumbnail image: Preparation of 4butylanilinebonded silica gel for the solidphase extraction of flavone glycosides
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