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Global quantification of phosphoproteins combining metabolic labeling and gel‐based proteomics in B. pumilus

Published: Oct 17, 2017

Author(s): Christian Hentschker, Carolin Dewald, Andreas Otto, Knut Büttner, Michael Hecker, Dörte Becher

Journal: ELECTROPHORESIS

Abstract Differential proteomics targeting the protein abundance is commonly used to follow changes in biological systems. Differences in localization and degree of post‐translational modifications of proteins including phosphorylations are of tremendous interest due to the anticipated role in molecular regulatory processes. Because of their particular low abundance in prokaryotes, identification and quantification of protein...

Read More thumbnail image: Global quantification of phosphoproteins combining metabolic labeling and gelbased proteomics in B_ pumilus

On gel electrophoresis of dielectric charged particles with hydrophobic surface: A combined theoretical and numerical study

Published: Oct 17, 2017

Author(s): Partha Sarathi Majee, Somnath Bhattacharyya, Partha Pratim Gopmandal, Hiroyuki Ohshima

Journal: ELECTROPHORESIS

Abstract A theoretical study on the gel electrophoresis of a charged particle incorporating the effects of dielectric polarization and surface hydrophobicity at the particle–liquid interface is made. A simplified model based on the weak applied field and low charge density assumption is also presented and compared with the full numerical model for a nonpolarizable particle to elucidate the nonlinear effects such as double layer...

Read More thumbnail image: On gel electrophoresis of dielectric charged particles with hydrophobic surface A combined theoretical and numerical study

Comparison of a polymeric pseudostationary phase in EKC with ODS stationary phase in RP‐HPLC

Published: Oct 17, 2017

Author(s): Xinjiong Ni, Min Zhang, Xiaoping Xing, Yuhua Cao, Guangqun Cao

Journal: ELECTROPHORESIS

Abstract Poly(stearyl methacrylate‐co‐methacrylic acid) (P(SMA‐co‐MAA)) was induced as pseudostationary phase (PSP) in electrokinetic chromatography (EKC). The n‐octadecyl groups in SMA were the same as that in octadecylsilane (ODS) C18 column. Thus, the present work focused on the comparison of selectivity between polymeric PSP and ODS stationary phase (SP), and the effect of organic modifiers on the selectivity of polymeric PSP and ODS ...

Read More thumbnail image: Comparison of a polymeric pseudostationary phase in EKC with ODS stationary phase in RPHPLC

Development of bufferless gel electrophoresis chip for easy preparation and rapid DNA separation

Published: Oct 17, 2017

Author(s): Sergiy Oleksandrov, Abdurazak Aman, Wanyoung Lim, Younghee Kim, Nam Ho Bae, Kyoung G. Lee, Seok Jae Lee, Sungsu Park

Journal: ELECTROPHORESIS

Abstract This work presents a handy, fast, and compact bufferless gel electrophoresis chip (BGEC), which consists of precast agarose gel confined in a disposable plastic body with electrodes. It does not require large volumes of buffer to fill reservoirs, or the process of immersing the gel in the buffer. It withstands voltages up to 28.4 V/cm, thereby allowing DNA separation within 10 min with a similar separation capability...

Read More thumbnail image: Development of bufferless gel electrophoresis chip for easy preparation and rapid DNA separation

Nonlinear classification of commercial Mexican tequilas

Published: Oct 17, 2017

Author(s): Jose Manuel Andrade, Davide Ballabio, Maria Paz Gómez‐Carracedo, Guadalupe Pérez‐Caballero

Journal: Journal of Chemometrics

Abstract Discriminant partial least squares (PLS‐DA)—a de facto standard classification method—was found to behave poorly when 3 classes of tequilas were modeled to study a collection of 170 commercial Mexican spirits measured by UV‐Vis spectroscopy. This result was compared with other linear and nonlinear supervised classification methods (PLS with variable selection by SRI index and genetic algorithms; kernel‐PLS—modified in this...

Read More thumbnail image: Nonlinear classification of commercial Mexican tequilas

Ensemble calibration for the spectral quantitative analysis of complex samples

Published: Oct 17, 2017

Author(s): Xihui Bian, Pengyao Diwu, Yirui Liu, Peng Liu, Qian Li, Xiaoyao Tan

Journal: Journal of Chemometrics

Abstract Ensemble strategies have gained increasing attention in multivariate calibration for quantitative analysis of complex samples. The aim of ensemble calibration is to obtain a more accurate, stable, and robust prediction by combining the predictions of multiple submodels. The generation and calibration of the training subsets, as well as the integration of the submodels, are three keys to the success of ensemble calibration....

Read More thumbnail image: Ensemble calibration for the spectral quantitative analysis of complex samples

Different localizations of drugs simultaneously administered in a strand of hair by micro‐segmental analysis

Published: Oct 17, 2017

Author(s): Kenji Kuwayama, Hajime Miyaguchi, Yuko T. Iwata, Tatsuyuki Kanamori, Kenji Tsujikawa, Tadashi Yamamuro, Hiroki Segawa, Hiroyuki Inoue

Journal: Drug Testing and Analysis

Abstract Segmental hair analysis is used to estimate the time of drug intake at monthly precision in drug‐related crimes. Previously, we advanced this analytical method to specify the day of drug intake by cutting a strand of hair into 0.4‐mm segments, which correspond to daily hair growth. Herein, we investigated the distributions of 7 compounds in a strand of hair using micro‐segmental analysis. Several strands of hair were...

Read More thumbnail image: Different localizations of drugs simultaneously administered in a strand of hair by microsegmental analysis

Automated curve fitting and unsupervised clustering of manganese oxide Raman responses

Published: Oct 17, 2017

Author(s): G. Mulè, C. Burlet, Y. Vanbrabant

Journal: Journal of Raman Spectroscopy

Natural manganese oxides characterization represents a challenge due to the broad variety of their structures and geochemical compositions along with a frequent poor crystallinity. This characterization requires the ability to conduct both a phase separation and a phase association operation. In this paper, the Raman spectra acquired on a selection of natural manganese oxide minerals are first processed with an automated curve‐fitting...

Read More thumbnail image: Automated curve fitting and unsupervised clustering of manganese oxide Raman responses

Ionic liquids: solvents and sorbents in sample preparation

Published: Oct 17, 2017

Author(s): Kevin D. Clark, Miranda N. Emaus, Marcelino Varona, Ashley N. Bowers, Jared L. Anderson

Journal: Journal of Separation Science

Abstract The applications of ionic liquids (ILs) and IL‐derived sorbents are rapidly expanding. By careful selection of the cation and anion components, the physicochemical properties of ILs can be altered to meet the requirements of specific applications. Reports of IL solvents possessing high selectivity for specific analytes are numerous and continue to motivate the development of new IL‐based sample preparation methods that are...

Read More thumbnail image: Ionic liquids solvents and sorbents in sample preparation

Development and validation of a subcritical 1,1,1,2‐tetrafluoroethane extraction technique: Gas chromatography–mass spectrometry method for the simultaneous determination of polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and polybrominated biphenyl ethers in aquatic products

Published: Oct 17, 2017

Author(s): Feiyue Xu, Xiaomei Feng, Nianrong Shi, Jiangbo Pan, Huashu Zhan, Yuqian Han

Journal: Journal of Separation Science

Abstract A simple, rapid, and green method was developed for the simultaneous analysis of polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and polybrominated biphenyl ethers in aquatic products using subcritical 1,1,1,2‐tetrafluoroethane extraction coupled with gas chromatography and mass spectrometry. Effects of the extraction temperature, pressure, and cosolvent volume on the extraction efficiency were investigated by...

Read More thumbnail image: Development and validation of a subcritical 1112tetrafluoroethane extraction technique Gas chromatographymass spectrometry method for the simultaneous determination of polycyclic aromatic hydrocarbons polychlorinated biphenyls and polybrominated biphenyl ethers in aquatic products

Improving the frame rates of Raman image sequences recorded with integral field spectroscopy using windowing and binning methods

Published: Oct 17, 2017

Author(s): Benito Moralejo, Elmar Schmälzlin, Daniel Bodenmüller, Thomas Fechner, Martin M. Roth

Journal: Journal of Raman Spectroscopy

Abstract Astrophysicists use Integral Field Spectroscopy to record spectrally resolved images of faint galaxies. Transferred to imaging Raman spectroscopy, this technique significantly accelerates the capture of Raman images as the simultaneous acquisition of multiple spectra makes scanning procedures obsolete. Furthermore, sequentially collected Raman images result in image sequences thus allowing to monitor Raman features in the...

Read More thumbnail image: Improving the frame rates of Raman image sequences recorded with integral field spectroscopy using windowing and binning methods

50 keV H + ion beam irradiation of Al doped ZnO thin films: Studies of radiation stability for device applications

Published: Oct 17, 2017

Author(s): Susanta Kumar Sahoo, Sutanu Mangal, D.K. Mishra, Udai P. Singh, Pravin Kumar

Journal: Surface and Interface Analysis

Thin films of Al doped ZnO (Al:ZnO) were deposited on two substrates (Si and glass) at room temperature and 300°C using DC magnetron sputtering. These films were bombarded with 50 keV H+ beam at several fluences. The pristine and ion beam irradiated films were analysed by X‐ray diffraction, Raman spectroscopy, scanning electron microscopy, and UV‐Vis spectroscopy. The X‐ray diffraction analysis, Hall measurements, Raman and UV‐Vis ...

Read More thumbnail image: 50keV H  ion beam irradiation of Al doped ZnO thin films Studies of radiation stability for device applications

SERS investigation of neocuproine adsorption on silver: Influence of electrode potential on methyl groups

Published: Oct 17, 2017

Author(s): Elena V. Solovyeva, Assima Rakhimbekova, Yulia V. Lanchuk, Liubov A. Myund, Anna S. Denisova

Journal: Journal of Raman Spectroscopy

Abstract Study of molecules adsorption on charged surfaces is important for biologically relevant substances where the potential at the interface such as living cell membrane is a significant parameter in the processes of their transportation or transmembrane penetration. In this work, a hybrid optical/electrochemical surface‐enhanced Raman scattering (SERS) technique was applied to get new insight into the adsorption state and...

Read More thumbnail image: SERS investigation of neocuproine adsorption on silver Influence of electrode potential on methyl groups

Relation between plasmonic tip emission and electromagnetic enhancement evidenced in tip‐enhanced Raman spectroscopy

Published: Oct 17, 2017

Author(s): J. Plathier, A. Merlen, A. Pignolet, A. Ruediger

Journal: Journal of Raman Spectroscopy

Abstract We report tip‐enhanced Raman spectroscopy measurements of single wall carbon nanotubes deposited on a barium titanate substrate. The tip‐enhanced Raman spectroscopy mappings demonstrate that the evolution of the gold tip enhanced luminescence is strongly correlated with the intensity of the Raman modes from the single wall carbon nanotubes and the substrate. As the tip emission is directly related to the plasmonic...

Read More thumbnail image: Relation between plasmonic tip emission and electromagnetic enhancement evidenced in tipenhanced Raman spectroscopy

Combined imaging and molecular techniques for evaluating microbial function and composition: A review

Published: Oct 17, 2017

Author(s): Christopher G. Weisener, Thomas Reid

Journal: Surface and Interface Analysis

In most cases, microbial function will determine the direction and onset of specific metabolic pathways as defined by their favorable thermodynamic outcome. Thus, the degree of chemical alteration in contact with minerals and bacteria can be directly proportional to the biological activity. This activity can influence redox conditions both from a localized perspective and global scale. Under these conditions, microscale mechanisms become...

Read More thumbnail image: Combined imaging and molecular techniques for evaluating microbial function and composition A review

Practical estimation of XPS binding energies using widely available quantum chemistry software

Published: Oct 17, 2017

Author(s): Sabrina Tardio, Peter J. Cumpson

Journal: Surface and Interface Analysis

Chemical shifts observed in high‐resolution X‐ray photoelectron spectroscopy (XPS) spectra are normally used to determine the chemical state of the elements of interest. Often, these shifts are small, or an element is present in several oxidation states in the same sample, so that interpretation of the spectra is difficult without good reference data on binding energies of the likely constituents. In many cases, reference spectra taken...

Read More thumbnail image: Practical estimation of XPS binding energies using widely available quantum chemistry software

Application of quantitative X‐ray photoelectron spectroscopy (XPS) imaging: investigation of Ni‐Cr‐Mo alloys exposed to crevice corrosion solution

Published: Oct 16, 2017

Author(s): Brad Kobe, Martin Badley, Jeffrey D. Henderson, Samantha Anderson, Mark C. Biesinger, Dave Shoesmith

Journal: Surface and Interface Analysis

This paper explores quantitative X‐ray photoelectron spectroscopy imaging on three different Ni‐Cr‐Mo Hastelloy® alloys (BC‐1, C‐22 and G‐30) with differing Cr and Mo contents. The alloys were subjected to a simulated critical crevice corrosion solution. Ni‐based alloys have been shown to exhibit excellent resistance to a range of corrosive media due to the formation of an inert oxide layer, primarily containing Cr and Mo. However, these...

Read More thumbnail image: Application of quantitative Xray photoelectron spectroscopy XPS imaging investigation of NiCrMo alloys exposed to crevice corrosion solution

The surface chemistry of metallurgical aluminas

Published: Oct 16, 2017

Author(s): Grant J. McIntosh, James B. Metson, Margaret M. Hyland

Journal: Surface and Interface Analysis

We have studied the evolution of surface basicity as a function of calcination temperature in a range of transition alumina‐rich analogues of metallurgical grade aluminas produced by calcination of Bayer gibbsite. Specific basicity, the number of basic sites per unit surface area, was determined by thermometric back‐titration and found to increase with calcination temperature up to ~700°C to 800°C, decreasing dramatically thereafter. The...

Read More thumbnail image: The surface chemistry of metallurgical aluminas

Insulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles

Published: Oct 16, 2017

Author(s): Michael Liem, Ching‐Seng Ang, Suresh Mathivanan

Journal: PROTEOMICS

Abstract Epidemiological studies suggest that diabetes and obesity increases the risk of colorectal cancer (CRC) and lowers the patient survival rate. An important attribute in diabetes and obesity is the presence of high levels of growth factors including insulin in blood which can activate the PI3K/Akt signalling pathway. Dysregulation of PI3K/Akt signalling pathway leads to sustained proliferative signals thereby allowing the...

Read More thumbnail image: Insulin Mediated Activation of PI3KAkt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles

Ion Mobility‐Enhanced Data‐Independent Acquisitions Enable a Deep Proteomic Landscape of Oligodendrocytes

Published: Oct 16, 2017

Author(s): Juliana S. Cassoli, Caroline Brandão‐Teles, Aline G. Santana, Gustavo H. M. F. Souza, Daniel Martins‐de‐Souza

Journal: PROTEOMICS

Abstract Oligodendrocytes are a type of neuroglia that provide trophic support and insulation to axons in the central nervous system. The genesis and maturation of oligodendrocytes are essential processes for myelination and the course of CNS development. Using ion mobility‐enhanced, data‐independent acquisitions and 2D‐nanoUPLC fractionation operating at nanoscale flow rates, we established a comprehensive data set of proteins...

Read More thumbnail image: Ion MobilityEnhanced DataIndependent Acquisitions Enable a Deep Proteomic Landscape of Oligodendrocytes
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