EarlyView Articles
The very latest articles from the scientific literature
on Wiley Online Library...
You can keep up-to date on these EarlyView articles, which focus on spectroscopy
and separation science:
Please check back soon.
Earlyview RSS Feed
rssFilter by Journal
Development and validation of a simple, sensitive and accurate LC‐MS/MS method for the determination of guanfacine, a selective α 2A ‐adrenergicreceptor agonist, in plasma and its application to a pharmacokinetic study
Published: Jul 8, 2013
Author(s): Xiaonan Li, Ning Li, Xiaolin Sun, Wei Yang, Yu Dai, Jie Xu, Wei Zhang, Chunfeng Wang, Suilou Wang, Xijing Chen
Journal: Biomedical Chromatography
ABSTRACT A simple, practical, accurate and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method was developed and fully validated for the quantitation of guanfacine in beagle dog plasma. After protein precipitation by acetonitrile, the analytes were separated on a C18 chromatographic column by methanol and water containing 0.1% (v/v) formic acid with a gradient elution. The subsequent detection utilized a mass...
Read More
Direct resolution and quantitative analysis of flurbiprofen enantiomers using microcrystalline cellulose triacetate plates: applications to the enantiomeric purity control and optical isomer determination in widely consumed drugs
Published: Jul 8, 2013
Author(s): M. Del Bubba, L. Checchini, L. Ciofi, S. Furlanetto, L. Lepri
Journal: Biomedical Chromatography
ABSTRACT Flurbiprofen enantiomers have very different pharmacological properties, since the (S)‐(+) form has a much higher anti‐inflammatory activity than the (R)‐(−) isomer, the latter being responsible for very undesirable side effects, such as gastrointestinal irritation. Based on the different biological properties of flurbiprofen enantiomers, the development of chiral chromatographic methods for the control of the enantiomeric purity is a...
Read More
Identification of (2‐aminopropyl)indole positional isomers in forensic samples
Published: Jul 8, 2013
Author(s): Kenneth R. Scott, John D. Power, Seán D. McDermott, John E. O'Brien, Brian N. Talbot, Michael G. Barry, Pierce V. Kavanagh
Journal: Drug Testing and Analysis
In 2012, 5‐(2‐aminopropyl)indole (5‐API, 5‐IT) was reported by Norwegian authorities to the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA) via the Early Warning System (EWS). The 3‐ isomer, 3‐(2‐aminopropyl)indole (3‐API, AMT, alpha‐methyltryptamine), has been available on the recreational drugs market for a somewhat longer time, having first been reported to the EMCDDA by Finnish authorities in 2001. Both isomers are available from...
Read More
The adverse health effects of chronic cannabis use
Published: Jul 8, 2013
Author(s): Wayne Hall, Louisa Degenhardt
Journal: Drug Testing and Analysis
This paper summarizes the most probable of the adverse health effects of regular cannabis use sustained over years, as indicated by epidemiological studies that have established an association between cannabis use and adverse outcomes; ruled out reverse causation; and controlled for plausible alternative explanations. We have also focused on adverse outcomes for which there is good evidence of biological plausibility. The focus is on...
Read More
The study of the structure and bioactivity of the B 2 O 3 • Na 2 O • P 2 O 5 system
Published: Jul 8, 2013
Author(s): I. J. Hidi, G. Melinte, R. Stefan, M. Bindea, L. Baia
Journal: Journal of Raman Spectroscopy
In this study, we have investigated calcium and silicate‐free samples over a wide compositional range in the xB2O3·30 Na2O·(70−x)P2O5 system, with 0 ≤ x ≤ 70 mol%, in order to determine the influence of the chemical composition on their structure and bioactive response in simulated body fluid. Information related to the chemical structures present in the network was obtained by means of Raman and infrared spectroscopy. For samples containing small...
Read More
Role of Phe‐D 5 isotopically labeled analogues of bradykinin on elucidation of its adsorption mode on Ag, Au, and Cu electrodes. Surface‐enhanced Raman spectroscopy studies
Published: Jul 8, 2013
Author(s): Edyta Proniewicz, Ilja Ignatjev, Gediminas Niaura, Dariusz Sobolewski, Adam Prahl, Leonard M. Proniewicz
Journal: Journal of Raman Spectroscopy
Raman (RS), surface‐enhanced Raman scattering (SERS), electrochemistry, and isotopic effect methods were used to characterize selective adsorption of two isotopically labeled bradykinin analogues, [(Phe‐D5)5]BK and [(Phe‐D5)8]BK, a hormone which is known to be involved in small‐cell and non‐small‐cell lung carcinoma and prostate cancer. The investigated analogues contain Phe residue, at position 5 or 8 in the amino acid sequence, substituted by...
Read More
Protein carbonylation as a major hallmark of oxidative damage: Update of analytical strategies
Published: Jul 7, 2013
Author(s): Maria Fedorova, Ravi Chand Bollineni, Ralf Hoffmann
Journal: Mass Spectrometry Reviews
Abstract Protein carbonylation, one of the most harmful irreversible oxidative protein modifications, is considered as a major hallmark of oxidative stress‐related disorders. Protein carbonyl measurements are often performed to assess the extent of oxidative stress in the context of cellular damage, aging and several age‐related disorders. A wide variety of analytical techniques are available to detect and...
Read More
Purification and characterization of transcription factors
Published: Jul 7, 2013
Author(s): L.I. Nagore, R.J. Nadeau, Q. Guo, Y.L.A. Jadhav, H.W. Jarrett, W.E. Haskins
Journal: Mass Spectrometry Reviews
Abstract Transcription factors (TFs) are essential for the expression of all proteins, including those involved in human health and disease. However, TFs are resistant to proteomic characterization because they are frequently masked by more abundant proteins due to the limited dynamic range of capillary liquid chromatography‐tandem mass spectrometry and protein database searching. Purification methods,...
Read More
Multiple‐echo diffusion tensor acquisition technique (MEDITATE) on a 3T clinical scanner
Published: Jul 5, 2013
Author(s): Steven H. Baete, Gene Cho, Eric E. Sigmund
Journal: NMR in Biomedicine
This article describes the concepts and implementation of an MRI method, the multiple‐echo diffusion tensor acquisition technique (MEDITATE), which is capable of acquiring apparent diffusion tensor maps in two scans on a 3T clinical scanner. In each MEDITATE scan, a set of RF pulses generates multiple echoes, the amplitudes of which are diffusion weighted in both magnitude and direction by a pattern of diffusion gradients. As a result,...
Read More
Molecularly imprinted polymer in microextraction by packed sorbent for the simultaneous determination of local anesthetics: lidocaine, ropivacaine, mepivacaine and bupivacaine in plasma and urine samples
Published: Jul 5, 2013
Author(s): Seyed Mosayeb Daryanavard, Amin Jeppsson‐Dadoun, Lars I. Andersson, Mahdi Hashemi, Anders Colmjsö, Mohamed Abdel‐Rehim
Journal: Biomedical Chromatography
ABSTRACT This study presents the use of molecularly imprinted polymer (MIP) as packing material for microextraction by packed syringe (MEPS) to achieve higher extraction selectivity. Pentycaine was used as template for MIP. Development and validation of the determination of lidocaine, ropivacaine, mepivacaine and bupivacaine in human plasma and urine samples utilizing MIP‐MEPS and liquid chromatography–tandem mass spectrometry...
Read More
Determination of chikusetsusaponin V and chikusetsusaponin IV in rat plasma by liquid chromatography–mass spectrometry and its application to a preliminary pharmacokinetic study
Published: Jul 5, 2013
Author(s): Dongli Qi, Xiaolin Yang, Jing Chen, Fei Li, Xiupu Shi, Chunfeng Zhang, Zhonglin Yang
Journal: Biomedical Chromatography
ABSTRACT A sensitive liquid chromatography–electrospray ionization–mass spectrometry method has been developed and validated for determination of two major bioactive saponins in rat plasma after oral administration of saponins extracted from Rhizoma Panacis Japonici, including chikusetsusaponin V and chikusetsusaponin IV for the first time. Akebia saponin D was used as the internal standard (IS). Plasma samples were prepared by...
Read More
SERS study on surface chain geometry of atactic poly (methyl methacrylate) film and nanosphere
Published: Jul 5, 2013
Author(s): Chenglong Hu, Shaoyun Chen, Weihong Zhang, Fangyan Xie, Jian Chen, Xudong Chen
Journal: Journal of Raman Spectroscopy
The surface chain geometry of atactic poly (methyl methacrylate) (a‐PMMA) film and nanosphere (NS) was revealed by surface‐enhanced Raman scattering (SERS) spectra. The Ag nanoparticles and nanoplates were prepared by electrochemical deposition and chemical synthesis for SERS substrates. The experimental results suggested that the molecular chain axis of a‐PMMA film adopted a trans‐conformation on bonding to Ag surface ascribed to the...
Read More
Adaptation of P haeobacter inhibens DSM 17395 to growth with complex nutrients
Published: Jul 5, 2013
Author(s): Hajo Zech, Michael Hensler, Sebastian Koßmehl, Katharina Drüppel, Lars Wöhlbrand, Kathleen Trautwein, Reiner Hulsch, Uwe Maschmann, Thomas Colby, Jürgen Schmidt, Richard Reinhardt, Kerstin Schmidt‐Hohagen, Dietmar Schomburg, Ralf Rabus
Journal: PROTEOMICS
Phaeobacter inhibens DSM 17395, a member of the Roseobacter clade, was studied for its adaptive strategies to complex and excess nutrient supply, here mimicked by cultivation with Marine Broth (MB). During growth in process‐controlled fermenters, P. inhibens DSM 17395 grew faster (3.6‐fold higher μmax) and reached higher optical densities (2.2‐fold) with MB medium, as compared to the reference condition of glucose‐containing mineral medium....
Read More
High‐permittivity solid ceramic resonators for high‐field human MRI
Published: Jul 4, 2013
Author(s): S. A. Aussenhofer, A. G. Webb
Journal: NMR in Biomedicine
The properties of a ceramic‐based annular dielectric resonator designed for 7 T MRI have been examined. Electromagnetic simulations and experimentally determined modal frequencies agree to within ~1%. The dependence of the resonance frequency of the degenerate quadrature HEM11 modes on hole diameter and shield diameter was also investigated. The constructed coil, with a 2.5 cm diameter hole, had an unloaded Q value of 400, which was...
Read More
High‐resolution NMR spectroscopy of human body fluids and tissues in relation to prostate cancer
Published: Jul 4, 2013
Author(s): Virendra Kumar, Durgesh K. Dwivedi, Naranamangalam R. Jagannathan
Journal: NMR in Biomedicine
High‐resolution NMR spectroscopic studies of prostate tissue extracts, prostatic fluid, seminal fluid, serum and urine can be used for the detection of prostate cancer, based on the differences in their metabolic profiles. Useful diagnostic information is obtained by the detection or quantification of as many metabolites as possible and comparison with normal samples. Only a few studies have shown the potential of high‐resolution in vitro...
Read More
Bayesian estimation of membership uncertainty in model‐based clustering
Published: Jul 4, 2013
Author(s): Liyuan Chen, Steven D. Brown
Journal: Journal of Chemometrics
We report the use of a cluster analysis method based on a multivariate mixture model, known as model‐based clustering, for overcoming the limitations of hierarchical clustering and relocation clustering. Unlike traditional clustering methods in which clusters are formed on the basis of intercluster distances, model‐based clustering classifies observations on the basis of probability estimated from Gaussian mixture modeling, and its...
Read More
Raman spectra of selected mineral phases of the Morasko iron meteorite
Published: Jul 2, 2013
Author(s): Ł. Karwowski, K. Helios, R. Kryza, A. Muszyński, P. Drożdżewski
Journal: Journal of Raman Spectroscopy
Selected minerals from oval inclusions (nodules) in the Morasko IAB iron meteorite have been investigated by electron microprobe (EMP) and micro‐Raman spectroscopy. Collected spectra of quartz, forsterite‐rich olivine, chromite, kosmochlor (Na–Cr‐rich clinopyroxene) and Na‐feldspar (albite) were compared with corresponding measurements of earth minerals and used for fast identification of meteorite fragments not EMP characterized. Some...
Read More
Serum lipid profiling of patients with chronic hepatitis B , cirrhosis, and hepatocellular carcinoma by ultra fast LC / IT ‐ TOF MS
Published: Jul 2, 2013
Author(s): Shili Chen, Peiyuan Yin, Xinjie Zhao, Wenbin Xing, Chunxiu Hu, Lina Zhou, Guowang Xu
Journal: ELECTROPHORESIS
In this study, an ultra fast LC/IT‐TOF MS (UFLC/IT‐TOF MS)‐based serum lipidomics method was employed to characterize the serum lipid profile of patients with chronic hepatitis B, cirrhosis, and hepatocellular carcinoma (HCC). After data collection and processing, 96 lipids including lysophosphatidylcholines, phosphatidylcholines, sphingomyelins, triacylglycerides, and cholesterol esters were identified and used for subsequent data...
Read More
Isomerization and epimerization of the aspartyl tetrapeptide A la‐ P he‐ A sp‐ G ly OH at p H 10—A CE study
Published: Jul 2, 2013
Author(s): Christin Brückner, Svenja‐Catharina Bunz, Diana Imhof, Christian Neusüß, Gerhard K. E. Scriba
Journal: ELECTROPHORESIS
Isomerization and enantiomerization of Asp in the tetrapeptide Ala‐Phe‐Asp‐GlyOH are studied at pH 10 and 80°C as well as 25°C. CE‐MS allowed the distinction between α‐Asp and β‐Asp linkages in degradation products based on the ratio of the b and y fragment ions. Besides isomerization and enantiomerization of Asp, enantiomerization of Ala and Phe was also observed at both temperatures by chiral amino acid HPLC analysis using Marfey's reagent for...
Read More
Preparation and characterization of copper nanoparticles surface‐capped by alkanethiols
Published: Jul 2, 2013
Author(s): Guangbin Yang, Zhanming Zhang, Shengmao Zhang, Laigui Yu, Pingyu Zhang, Yanchao Hou
Journal: Surface and Interface Analysis
Cu nanoparticles surface‐capped by alkanethiols were synthesized using ligand exchange method in a two‐phase system. The effects of synthetic conditions, including the pH value of CuSO4 solution, the ratio of cetyltrimethyl ammonium bromide to CuSO4, and reaction temperature, on the size and shape of as‐synthesized Cu nanoparticles were investigated. As‐synthesized Cu nanoparticles surface‐capped by alkanethiols with different chain lengths (CxS‐Cu)...
Read More
