EarlyView Articles

Skip to Navigation

The very latest articles from the scientific literature
on Wiley Online Library...


You can keep up-to date on these EarlyView articles, which focus on spectroscopy
and separation science:


Please check back soon.

Wiley Online Library

Earlyview RSS Feed

rss

Filter by Journal

Browse By Journal

The advances of electromigration techniques applied for alkaloid analysis

Published: Jul 1, 2013

Author(s): Szymon Dziomba, Mariusz Belka, Piotr Kowalski, Alina Plenis, Tomasz Bączek

Journal: Biomedical Chromatography

ABSTRACT Alkaloids are natural products of metabolism found mainly in plants. Their diversified pharmacological activities as medical agents and extensive occurrence in nutritional products demand the development of reliable and sensitive analytical tools. This review presents the developments in the field of electromigration techniques and specifically capillary and microchip electrophoresis. We include the main aspects of interest...

Read More thumbnail image: The advances of electromigration techniques applied for alkaloid analysis

Screening and determination for potential α ‐glucosidase inhibitory constituents from Dalbergia odorifera T. Chen using ultrafiltration‐LC/ESI‐MS n

Published: Jul 1, 2013

Author(s): Chunfang Zhao, Yongqiang Liu, Dengli Cong, Hui Zhang, Jiajia Yu, Ying Jiang, Xiuyue Cui, Jiaming Sun

Journal: Biomedical Chromatography

ABSTRACT In the present study, it was demonstrated that ethyl acetate soluble fraction partitioned from heartwood of Dalbergia odorifera T. Chen (HEF) had a remarkable inhibitory effect on α‐glucosidase. Therefore HEF was selected as a starting material for screening the potential α‐glucosidase inhibitors using ultrafiltration liquid chromatography/mass spectrometry (UF‐LC/MS). Twenty‐six compounds were identified with analysis of LC/MS. UF assay indicated...

Read More thumbnail image: Screening and determination for potential  glucosidase inhibitory constituents from Dalbergia odorifera T_ Chen using ultrafiltrationLCESIMS n

Laser wavelength dependence of background fluorescence in Raman spectroscopic analysis of synovial fluid from symptomatic joints

Published: Jul 1, 2013

Author(s): Shan Yang, Bolan Li, Mikhail N. Slipchenko, Anna Akkus, Nora G. Singer, Yener N. Yeni, Ozan Akkus

Journal: Journal of Raman Spectroscopy

Gout is a disease process where the nucleation and growth of crystals in the synovial fluid of joints elicit painful arthritis‐like symptoms. Raman spectroscopy is evolving as a potential diagnostic tool in identifying such crystals; however, attainment of sufficient Raman signal while overcoming the background fluorescence remains as a major challenge. The current study focused on assessing whether excitation in 532–700 nm range will...

Read More thumbnail image: Laser wavelength dependence of background fluorescence in Raman spectroscopic analysis of synovial fluid from symptomatic joints

Characterization and quantification of 10‐hydroxycamptothecine in Camptotheca acuminate and its medicinal preparation by liquid chromatography–ion trap mass spectrometry

Published: Jul 1, 2013

Author(s): Qinhua Chen, Guangmin Pan, Lin Xiong, Hongsheng He, Hangdong Yang

Journal: Biomedical Chromatography

ABSTRACT A rapid and sensitive method for the identification and quantification of 10‐hydroxycamptothecine (HCPT) in Camptotheca acuminata Decne is described. The HCPT standard solution was directly infused into the ion trap mass spectrometers (IT/MS) for collecting the MSn spectra. The electrospray ionization (ESI) mass spectral fragmentation pathway of HCPT was proposed and the ESI‐MSn fragmentation behavior of HCPT was deduced in detail. The major...

Read More thumbnail image: Characterization and quantification of 10hydroxycamptothecine in Camptotheca acuminate and its medicinal preparation by liquid chromatographyion trap mass spectrometry

Determination of 3‐ α ‐hydroxytibolone in human plasma by LC‐MS/MS: application for a pharmacokinetic study after administration of a tibolone formulation

Published: Jul 1, 2013

Author(s): Fabiana Fernandes de Santana e Silva Cardoso, Isabela Costa César, Iram Moreira Mundim, Leonardo de Souza Teixeira, Enikson Pontes Silva, Ricardo Rodrigues Bonfim, Sandro Antônio Gomes, Denys Pires Ferreira, Aderimar Rogério Batista Lopes, Helifas Duarte Pascoal, Weidson Carlo Souza, Gerson Antônio Pianetti

Journal: Biomedical Chromatography

ABSTRACT A new method was developed for the quantitation of 3‐α‐hydroxy tibolone, in human plasma, after oral administration of a tablet formulation containing tibolone (2.5 mg). 3‐α‐Hydroxy tibolone was extracted by a liquid–liquid procedure, using cyproterone acetate as internal standard and chlorobutane as extraction solvent. After extraction, samples were submitted to a derivatization step with p‐toluenesulfonyl isocyanate. A mobile phase consisting of...

Read More thumbnail image: Determination of 3  hydroxytibolone in human plasma by LCMSMS application for a pharmacokinetic study after administration of a tibolone formulation

Efficient chromatographic separation of intact proteins derivatized with a fluorogenic reagent for proteomics analysis

Published: Jul 1, 2013

Author(s): Tomoko Ichibangase, Itaru Yazawa, Kazuhiro Imai

Journal: Biomedical Chromatography

ABSTRACT To achieve more efficient separation of intact proteins for proteomics applications, three columns of differing diameters (4.0, 4.6 and 6.0 mm internal diameter) were chosen for comparison and investigated to identify optimal conditions. The column with the largest diameter gave the largest peak capacity, showing the efficient separation of intact proteins, such as two protein standards, glutathione S‐transferase and...

Read More thumbnail image: Efficient chromatographic separation of intact proteins derivatized with a fluorogenic reagent for proteomics analysis

Quantitative determination of d‐ and l‐threo enantiomers of methylphenidate in brain tissue by liquid chromatography–mass spectrometry

Published: Jul 1, 2013

Author(s): Carolyn C. Combs, Erin L. Hankins, Cara L. Copeland, Stacy D. Brown, Brooks B. Pond

Journal: Biomedical Chromatography

ABSTRACT Methylphenidate, a psychostimulant used for the treatment of attention deficit hyperactivity disorder and narcolepsy, is administered as a 50:50 racemic mixture, despite the fact that d‐methylphenidate has been shown to have greater pharmacologic activity. This paper presents a validated LC‐MS/MS approach to separation and quantification of methylphenidate enantiomers using a vancomycin column and triethylammonium acetate...

Read More thumbnail image: Quantitative determination of d and lthreo enantiomers of methylphenidate in brain tissue by liquid chromatographymass spectrometry

Validation of a new HPLC‐UV method for determination of the antibiotic linezolid in human plasma and in bronchoalveolar lavage

Published: Jul 1, 2013

Author(s): Serena Fortuna, Gennaro De Pascale, Enzo Ragazzoni, Massimo Antonelli, Pierluigi Navarra

Journal: Biomedical Chromatography

ABSTRACT A rapid and selective HPLC‐UV method was developed for the quantification of linezolid (LNZ) in human plasma and bronchoalveolar lavage (BAL) at the concentrations associated with therapy. Plasma samples were extracted by solid‐phase extraction followed by evaporation to dryness and reconstitution in mobile phase solution. The chromatographic separation was carried out on a C18 column with an isocratic mobile phase...

Read More thumbnail image: Validation of a new HPLCUV method for determination of the antibiotic linezolid in human plasma and in bronchoalveolar lavage

Simultaneous quantitative analyses of indole and oxindole alkaloids of Uncaria Hook in rat plasma and brain after oral administration of the traditional Japanese medicine Yokukansan using high‐performance liquid chromatography with tandem mass spectrometr

Published: Jul 1, 2013

Author(s): Hirotaka Kushida, Miwako Fukutake, Masahiro Tabuchi, Takao Katsuhara, Hiroaki Nishimura, Yasushi Ikarashi, Masanao Kanitani, Yoshio Kase

Journal: Biomedical Chromatography

ABSTRACT Uncaria Hook (UH) alkaloids are involved in the beneficial effects of Yokukansan. However, the pharmacokinetics of UH alkaloids after oral administration of Yokukansan has not yet been sufficiently investigated. Therefore, we developed and validated a sensitive and specific high‐performance liquid chromatography with tandem mass spectrometry (LC/MS/MS) method for the simultaneous quantitation of seven UH alkaloids...

Read More thumbnail image: Simultaneous quantitative analyses of indole and oxindole alkaloids of Uncaria Hook in rat plasma and brain after oral administration of the traditional Japanese medicine Yokukansan using highperformance liquid chromatography with tandem mass spectrometr

Modification of cysteine residues by cyclopentenone prostaglandins: Interplay with redox regulation of protein function

Published: Jul 1, 2013

Author(s): Clara L. Oeste, Dolores Pérez‐Sala

Journal: Mass Spectrometry Reviews

Abstract Cyclopentenone prostaglandins (cyPG) are endogenous lipid mediators involved in the resolution of inflammation and the regulation of cell proliferation and cellular redox status. Upon exogenous administration they have shown beneficial effects in models of inflammation and tissue injury, as well as potential antitumoral actions, which have raised a considerable interest in their study for the...

Read More thumbnail image: Modification of cysteine residues by cyclopentenone prostaglandins Interplay with redox regulation of protein function

Quantitative analysis of heavy elements and semi‐quantitative evaluation of heavy mineral compositions of sediments in Japan for construction of a forensic soil database using synchrotron radiation X‐ray analyses

Published: Jun 28, 2013

Author(s): Izumi Nakai, Shunsuke Furuya, Willy Bong, Yoshinari Abe, Keiichi Osaka, Takuya Matsumoto, Masayoshi Itou, Atsuyuki Ohta, Toshio Ninomiya

Journal: X-Ray Spectrometry

We have started to construct a nationwide forensic soil sediment database for Japan, based on the heavy mineral and trace heavy element compositions of stream sediments collected at 3024 points across Japan. The data were measured by high‐resolution synchrotron radiation X‐ray powder diffraction (SR‐XRD) and high‐energy SR X‐ray fluorescence analysis (XRF) using the SR source of SPring‐8, the SR radiation facility in Japan. The automated...

Read More thumbnail image: Quantitative analysis of heavy elements and semiquantitative evaluation of heavy mineral compositions of sediments in Japan for construction of a forensic soil database using synchrotron radiation Xray analyses

High‐resolution imaging of magnetisation transfer and nuclear Overhauser effect in the human visual cortex at 7 T

Published: Jun 26, 2013

Author(s): Olivier Mougin, Matthew Clemence, Andrew Peters, Alain Pitiot, Penny Gowland

Journal: NMR in Biomedicine

The aim of this study was to optimise a pulse sequence for high‐resolution imaging sensitive to the effects of conventional macromolecular magnetisation transfer (MTm) and nuclear Overhauser enhancement (NOE), and to use it to investigate variations in these parameters across the cerebral cortex. A high‐spatial‐resolution magnetisation transfer‐prepared turbo field echo (MT‐TFE) sequence was designed to have high sensitivity to MTm and...

Read More thumbnail image: Highresolution imaging of magnetisation transfer and nuclear Overhauser effect in the human visual cortex at 7 T

Electrochemically controlled detection of adrenaline on poly(2‐aminobenzylamine) thin films by surface plasmon resonance spectroscopy and quartz crystal microbalance

Published: Jun 26, 2013

Author(s): Sopis Chuekachang, Rapiphun Janmanee, Akira Baba, Sukon Phanichphant, Saengrawee Sriwichai, Kazunari Shinbo, Keizo Kato, Futao Kaneko, Nobuko Fukuda, Hirobumi Ushijima

Journal: Surface and Interface Analysis

In this study, we present an electrochemically controlled surface plasmon resonance (EC‐SPR) biosensor to detect adrenaline on poly(2‐aminobenzylamine) (P2ABA) thin films. The P2ABA thin films are stable and display electroactivity in a neutral PBS solution. Specific detection of adrenaline was performed on P2ABA thin films because the benzylamine groups in the P2ABA structure could specifically react with adrenalines. Adrenaline was...

Read More thumbnail image: Electrochemically controlled detection of adrenaline on poly2aminobenzylamine thin films by surface plasmon resonance spectroscopy and quartz crystal microbalance

Advanced hyphenated chromatographic‐mass spectrometry in mycotoxin determination: Current status and prospects

Published: Jun 26, 2013

Author(s): Peiwu Li, Zhaowei Zhang, Xiaofeng Hu, Qi Zhang

Journal: Mass Spectrometry Reviews

Abstract Mass spectrometric techniques are essential for advanced research in food safety and environmental monitoring. These fields are important for securing the health of humans and animals, and for ensuring environmental security. Mycotoxins, toxic secondary metabolites of filamentous fungi, are major contaminants of agricultural products, food and feed, biological samples, and the environment as a whole....

Read More thumbnail image: Advanced hyphenated chromatographicmass spectrometry in mycotoxin determination Current status and prospects

Salbutamol extraction from urine and its stability in different solutions: identification of methylation products and validation of a quantitative analytical method

Published: Jun 26, 2013

Author(s): Bruno Carius Garrido, Mayra Leal Chrisóstomo Silva, Ricardo Moreira Borges, Monica Costa Padilha, Francisco Radler Aquino Neto

Journal: Biomedical Chromatography

ABSTRACT Salbutamol is commonly used in asthma treatment, being considered a short‐effect bronchodilator. This drug poses special interest in certain fields of chemical analysis, such as food, clinical and doping analyses, in which it needs to be analyzed with quantitative precision and accuracy. Salbutamol, however, is known to degrade under certain conditions and this is critical if quantitative results must be generated. The...

Read More thumbnail image: Salbutamol extraction from urine and its stability in different solutions identification of methylation products and validation of a quantitative analytical method

In vivo MRI analysis of depth‐dependent ultrastructure in human knee cartilage at 7 T

Published: Jun 25, 2013

Author(s): Nikita Garnov, Wilfried Gründer, Gregor Thörmer, Robert Trampel, Robert Turner, Thomas Kahn, Harald Busse

Journal: NMR in Biomedicine

Signal intensities of T2‐weighted magnetic resonance images depend on the local fiber arrangement in hyaline cartilage. The aims of this study were to determine whether angle‐sensitive MRI at 7 T can be used to quantify the cartilage ultrastructure of the knee in vivo and to assess potential differences with age. Ten younger (21–30 ) and ten older (55–76 years old) healthy volunteers were imaged with a T2‐weighted spin‐echo...

Read More thumbnail image: In vivo MRI analysis of depthdependent ultrastructure in human knee cartilage at 7T

Determination of the stress biomarker corticosterone in serum of tumor‐bearing mice by surrogate‐based liquid chromatography–tandem mass spectrometry

Published: Jun 25, 2013

Author(s): Lei Kang, Tao Jiang, Xinxing Ge, Liang Peng, Ying Xie, Xin Luan, Huafang Li, Zhengxing Rong, Hong Qi, Hongzhuan Chen

Journal: Biomedical Chromatography

ABSTRACT A simple, robust and specific liquid chromatography tandem mass spectrometry (LC‐MS/MS) method was developed and validated to determine the concentration of corticosterone (Cort) which is usually regarded as a stress biomarker in mouse serum. Since Cort is an endogenous hormone, a ‘surrogate analyte’ strategy was adopted using the stable isotope‐deuterated corticosterone as a surrogate of the authentic analyte to generate...

Read More thumbnail image: Determination of the stress biomarker corticosterone in serum of tumorbearing mice by surrogatebased liquid chromatographytandem mass spectrometry

Using microbubbles as an MRI contrast agent for the measurement of cerebral blood volume

Published: Jun 24, 2013

Author(s): Shin‐Lei Peng, Fu‐Nien Wang, Chung‐Hsin Wang, Hsu‐Hsia Peng, Chih‐Tien Lu, Chih‐Kuang Yeh

Journal: NMR in Biomedicine

The susceptibility differences at the gas–liquid interface of microbubbles (MBs) allow their use as an intravascular susceptibility contrast agent for in vivo MRI. However, the characteristics of MBs are very different from those of the standard gadolinium‐diethylenetriaminepentaacetic acid (Gd‐DPTA) contrast agent, including the size distribution and hemodynamic properties, which could influence MRI outcomes. Here, we investigate...

Read More thumbnail image: Using microbubbles as an MRI contrast agent for the measurement of cerebral blood volume

Enhancing the [ 13 C]bicarbonate signal in cardiac hyperpolarized [1‐ 13 C]pyruvate MRS studies by infusion of glucose, insulin and potassium

Published: Jun 24, 2013

Author(s): Mette Hauge Lauritzen, Christoffer Laustsen, Sadia Asghar Butt, Peter Magnusson, Lise Vejby Søgaard, Jan Henrik Ardenkjær‐Larsen, Per Åkeson, C. Laustsen, J. H. Ardenkjær‐Larsen, J. H. Ardenkjær‐Larsen

Journal: NMR in Biomedicine

A change in myocardial metabolism is a known effect of several diseases. MRS with hyperpolarized 13C‐labelled pyruvate is a technique capable of detecting changes in myocardial pyruvate metabolism, and has proven to be useful for the evaluation of myocardial ischaemia in vivo. However, during fasting, the myocardial glucose oxidation is low and the fatty acid oxidation (β‐oxidation) is high, which complicates the interpretation of pyruvate metabolism...

Read More thumbnail image: Enhancing the  13 Cbicarbonate signal in cardiac hyperpolarized 1 13 Cpyruvate MRS studies by infusion of glucose insulin and potassium

Characterization of synthetic routes to ‘Bromo‐DragonFLY’ and benzodifuranyl isopropylamine homologues utilizing ketone intermediates. Part 1: Synthesis of ketone precursors

Published: Jun 24, 2013

Author(s): Richard E. O'Connor, John J. Keating

Journal: Drug Testing and Analysis

Bromo‐DragonFLY (BDF) and many of its analogues are misused as recreational drugs due to their potency as psychoactive substances. To date, none of the published routes to these designer amphetamines have exploited a ketone intermediate. It is well known that benzyl methyl ketone (BMK) can be employed as a precursor in the synthesis of amphetamine. Similarly, it is reasonable to assume that ketone precursors may potentially be utilized in...

Read More thumbnail image: Characterization of synthetic routes to BromoDragonFLY and benzodifuranyl isopropylamine homologues utilizing ketone intermediates_ Part 1 Synthesis of ketone precursors
Page:   Prev 6 7 8 9 10 11 12 13 14 15 16 Next

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2013 John Wiley & Sons, Inc. All Rights Reserved