EarlyView Articles

Skip to Navigation

The very latest articles from the scientific literature
on Wiley Online Library...


You can keep up-to date on these EarlyView articles, which focus on spectroscopy
and separation science:

Earlyview RSS Feed

rss

Filter by Journal

Browse By Journal

Development and applications of nonlinear optical spectroscopy: 16th ECONOS/36th ECW meeting in Jena (Germany)

Published: Jun 8, 2018

Author(s): Michael Schmitt, Jürgen Popp, Johannes Kiefer, Julian Moger

Journal: Journal of Raman Spectroscopy

Read More thumbnail image: Development and applications of nonlinear optical spectroscopy 16th ECONOS36th ECW meeting in Jena Germany

Metabolomic comparison between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris

Published: Jun 7, 2018

Author(s): Lin Chen, Yuetao Liu, Qingfeng Guo, Qingxia Zheng, Wancun Zhang

Journal: Biomedical Chromatography

Abstract A systematic study on the metabolome differences between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris was conducted using liquid chromatography−mass spectrometry. Principal component analysis and orthogonal projection on latent structure‐discriminant analysis results showed that C. militaris grown on solid rice medium (R‐CM) and C. militaris grown on tussah pupa (T‐CM) evidently separated and individually separated from...

Read More thumbnail image: Metabolomic comparison between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris

Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Förster resonance energy transfer

Published: Jun 7, 2018

Author(s): Vladimíra Datinská, Karel Klepárník, Barbora Belšánová, Marek Minárik, František Foret

Journal: Journal of Separation Science

Abstract The synthesis and determination of the structure of a Förster resonance energy transfer probe intended for the detection of specific nucleic acid sequences are described here. The probe is based on the hybridization of oligonucleotide modified quantum dots with a fluorescently labeled nucleic acid sample resulting in changes of the fluorescence emission due to the energy transfer effect. The stoichiometry distribution of...

Read More thumbnail image: Capillary electrophoresis a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer

Preparative free‐flow electrophoresis, a versatile technology complementing gradient centrifugation in the isolation of highly purified cell organelles

Published: Jun 7, 2018

Author(s): Markus Islinger, Robert Wildgruber, Alfred Völkl

Journal: ELECTROPHORESIS

Abstract Free‐flow electrophoresis (FFE) exploits differences in the overall charge of bio‐particles separating cells, organelles, macromolecules, ions, etc. according to their distinct electrophoretic mobility and isoelectric point (pI) values. Indeed, around a neutral pH organelles usually exhibit a negative surface charge, migrating in an electric field from the cathode toward the anode. Since its introduction more than five decades ago by...

Read More thumbnail image: Preparative freeflow electrophoresis a versatile technology complementing gradient centrifugation in the isolation of highly purified cell organelles

Optimization of artemisinin extraction from Artemisia annua L. with supercritical carbon dioxide + ethanol using response surface methodology

Published: Jun 7, 2018

Author(s): Ozan Nazim Ciftci, Jessica Cahyadi, Selma E. Guigard, Marleny D. A. Saldaña

Journal: ELECTROPHORESIS

Abstract Malaria is a high priority life‐threatening public health concern in developing countries, and therefore there is a growing interest to obtain artemisinin for the production of artemisinin‐based combination therapy products. In this study, artemisinin was extracted from the Artemisia annua L. plant using supercritical carbon dioxide (SC‐CO2) modified with ethanol. Response surface methodology based on central composite rotatable design...

Read More thumbnail image: Optimization of artemisinin extraction from Artemisia annua L_ with supercritical carbon dioxide  ethanol using response surface methodology

A combined experimental and theoretical approach to determine X‐ray atomic fundamental quantities of tin

Published: Jun 7, 2018

Author(s): Y. Ménesguen, M.‐C. Lépy, J. M. Sampaio, J. P. Marques, F. Parente, M. Guerra, P. Indelicato, J. P. Santos, P. Hönicke, B. Beckhoff

Journal: X-Ray Spectrometry

The knowledge of atomic fundamental parameters, such as the mass attenuation coefficients or fluorescence yields with low uncertainties, is of decisive importance in elemental quantification involving X‐ray fluorescence analysis techniques. For example, several databases giving the mass attenuation coefficients are accessible and frequently used within a large community of users. These compilations are most often in good agreement for...

Read More thumbnail image: A combined experimental and theoretical approach to determine Xray atomic fundamental quantities of tin

Investigation of the pigment use in the Tomb of the Reliefs and other tombs in the Etruscan Banditaccia Necropolis

Published: Jun 7, 2018

Author(s): Matthias Alfeld, Cecilia Baraldi, Maria Cristina Gamberini, Philippe Walter

Journal: X-Ray Spectrometry

The pigment use in the Tomb of the Reliefs (fourth century BC) and four other tombs (seventh to fourth century BC) in the Etruscan Banditaccia Necropolis near Cerveteri, Italy, has been investigated. We made use of X‐ray fluorescence (XRF) and Raman spot analysis, XRF imaging, infrared luminescence and photography supported by Dstretch contrast enhancement. We identified the use of haematite, goethite, calcite, carbon black, manganese...

Read More thumbnail image: Investigation of the pigment use in the Tomb of the Reliefs and other tombs in the Etruscan Banditaccia Necropolis

Metabolic profiles of neratinib in rat by using ultra‐high‐performance liquid chromatography coupled with diode array detector and Q‐Exactive Orbitrap tandem mass spectrometry

Published: Jun 5, 2018

Author(s): Wen Liu, Sha Li, Yangke Wu, Xiao Yan, Y.‐M. Zhu, J.‐H. Huang, Zhuo Chen

Journal: Biomedical Chromatography

Abstract Neratinib is a tyrosine kinase inhibitor that has been approved by the US Food and Drug Administration for the treatment of breast cancer. However, its metabolism remains unknown. This study was carried out to investigate the in vitro and in vivo metabolism of neratinib using an UHPLC‐DAD‐Q Exactive Orbitrap‐MS instrument with dd‐MS2 on‐line data acquisition mode. The post‐acquisition data was processed using MetWorks software. Under the current...

Read More thumbnail image: Metabolic profiles of neratinib in rat by using ultrahighperformance liquid chromatography coupled with diode array detector and QExactive Orbitrap tandem mass spectrometry

Evaluation of needle trap micro‐extraction and solid‐phase micro‐extraction: Obtaining comprehensive information on volatile emissions from in vitro cultures

Published: Jun 5, 2018

Author(s): Peter Oertel, Andreas Bergmann, Sina Fischer, Phillip Trefz, Anne Küntzel, Petra Reinhold, Heike Köhler, Jochen K. Schubert, Wolfram Miekisch

Journal: Biomedical Chromatography

Abstract Volatile organic compounds (VOCs) emitted from in vitro cultures may reveal information on species and metabolism. Owing to low nmol L−1 concentration ranges, pre‐concentration techniques are required for gas chromatography–mass spectrometry (GC–MS) based analyses. This study was intended to compare the efficiency of established micro‐extraction techniques – solid‐phase micro‐extraction (SPME) and needle‐trap micro‐extraction (NTME) –...

Read More thumbnail image: Evaluation of needle trap microextraction and solidphase microextraction Obtaining comprehensive information on volatile emissions from in vitro cultures

Chemical Proteomics of Host–Microbe Interactions

Published: Jun 5, 2018

Author(s): Megan H Wright

Journal: PROTEOMICS

Abstract The dynamic proteome plays numerous roles in the interactions of microbes—whether they are invading pathogens or symbiotic organisms—and their hosts. Host and microbe sense, respond, and manipulate each other's biology via a multitude of mechanisms, resulting in alterations in protein expression or posttranslational modification that influence protein localization, activity, or binding partners. The intrinsic, temporal, and...

Read More thumbnail image: Chemical Proteomics of HostMicrobe Interactions

Neuroproteins in Cancer: Assumed Bystanders Become Culprits

Published: Jun 5, 2018

Author(s): Xiang Li, Matt D. Dun, Sam Faulkner, Hubert Hondermarck

Journal: PROTEOMICS

Abstract Recent breakthrough discoveries have highlighted the stimulatory role of nerves in cancer initiation and progression, through the release of neurotransmitters and growth factors by nerve terminals in the tumor microenvironment. Intriguingly, neuroproteins such as neuronal membrane proteins, synaptic proteins, neurotransmitters, and neurotrophic growth factors as well as their corresponding receptors, to name only a few, are...

Read More thumbnail image: Neuroproteins in Cancer Assumed Bystanders Become Culprits

Development of micellar electrokinetic chromatography method for the determination of three defined impurities in indomethacin

Published: Jun 5, 2018

Author(s): Petra Riasová, Dagmar Doubková, Lucie Pincová, Ondřej Jung, Miroslav Polášek, Pavel Jáč

Journal: ELECTROPHORESIS

Abstract A micellar electrokinetic chromatography method for the determination of indomethacin impurities (4‐chlorobenzoic acid, 5‐methoxy‐2‐methyl‐3‐indoleacetic acid, and 3,4‐dichloroindomethacin) has been developed. A 64.5/56‐cm fused silica 50 μm id capillary with extended light path (150 μm id) detection window was used. Internal standard was 1‐naphthylacetic acid. The analytes were separated at 30 kV with ...

Read More thumbnail image: Development of micellar electrokinetic chromatography method for the determination of three defined impurities in indomethacin

Comparison of protein precipitation methods from adipose tissue using difference gel electrophoresis

Published: Jun 5, 2018

Author(s): Hicham Benabdelkamel, Afshan Masood, Ibrahim O Alanazi, Assim A Alfadda

Journal: ELECTROPHORESIS

Abstract Proteomic methods have great potential to aid our understanding of the functional and pathological roles of adipose tissue. A critical initial step in the proteomic studies is the efficient isolation of proteins before conducting detailed analysis. In this study, three different methods were used for precipitating proteins; we analyzed samples from visceral adipose tissue, subcutaneous adipose tissue, and stromal visceral...

Read More thumbnail image: Comparison of protein precipitation methods from adipose tissue using difference gel electrophoresis

Determination of brompheniramine enantiomers in rat plasma by cation‐selective exhaustive injection and sweeping cyclodextrin modified electrokinetic chromatography method

Published: Jun 5, 2018

Author(s): Yaqi Yao, Yu Zhao, Xue Zhang, Jia Yu, Xingjie Guo

Journal: ELECTROPHORESIS

Abstract A method consisting of cation‐selective exhaustive injection and sweeping (CSEI‐sweeping) as online preconcentration followed by a cyclodextrin modified electrokinetic chromatography (CDEKC) enantioseparation has been developed for the simultaneous determination of two brompheniramine enantiomers in rat plasma. In this method, analytes were electrokinetically injected at a voltage of 8 kV for 80 s in a fused‐silica...

Read More thumbnail image: Determination of brompheniramine enantiomers in rat plasma by cationselective exhaustive injection and sweeping cyclodextrin modified electrokinetic chromatography method

Comparative two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) of human milk to identify dysregulated proteins in breast cancer

Published: Jun 5, 2018

Author(s): Roshanak Aslebagh, Devika Channaveerappa, Kathleen F. Arcaro, Costel C. Darie

Journal: ELECTROPHORESIS

Abstract Breast cancer (BC) remains a major cause of mortality, and early detection is considered important for reducing BC‐associated deaths. Early detection of BC is challenging in young women, due to the limitations of mammography on the dense breast tissue of young women. We recently reported results of a pilot proteomics study, using one‐dimensional polyacrylamide gel electrophoresis (1D‐PAGE) and mass spectrometry (MS) to...

Read More thumbnail image: Comparative twodimensional polyacrylamide gel electrophoresis 2DPAGE of human milk to identify dysregulated proteins in breast cancer

Proteinase pattern of honeybee prepupae from healthy and American Foulbrood infected bees investigated by zymography

Published: Jun 5, 2018

Author(s): Antonio Felicioli, Barbara Turchi, Filippo Fratini, Matteo Giusti, Roberta Nuvoloni, Francesca Romana Dani, Simona Sagona

Journal: ELECTROPHORESIS

Abstract American foulbrood disease (AFB) is the main devastating disease that affects honeybees’ brood, caused by Paenibacillus larvae. The trend of the research on AFB has addressed the mechanisms by which P. larvae bacteria kill honeybee larvae. Since prepupae could react to the infection of AFB by increasing protease synthesis, the aim of this work was to compare protease activity in worker prepupae belonging to healthy colonies and to colonies...

Read More thumbnail image: Proteinase pattern of honeybee prepupae from healthy and American Foulbrood infected bees investigated by zymography

High‐resolution separation of monoclonal antibodies mixtures and their charge variants by an alternative and generic CZE method

Published: Jun 5, 2018

Author(s): Alexandre Goyon, Yannis Nicolas Francois, Olivier Colas, Alain Beck, Jean Luc Veuthey, Davy Guillarme

Journal: ELECTROPHORESIS

Abstract The determination of mAb critical quality attributes (CQA) is crucial for their successful application in health diseases. A generic CZE method was developed for the high‐resolution separation of various mAb charge variants, which are often recognized as important CQA. A dynamic coating of the capillary was obtained with polyethylene oxide (PEO), whereas Bis‐Tris allowed the analysis of mAbs under native conditions at pH...

Read More thumbnail image: Highresolution separation of monoclonal antibodies mixtures and their charge variants by an alternative and generic CZE method

Current state‐of‐art of STR sequencing in forensic genetics

Published: Jun 5, 2018

Author(s): Antonio Alonso, Pedro Alberto Barrio, Petra Müller, Steffi Köcher, Burkhard Berger, Pablo Martin, Martin Bodner, Sascha Willuweit, Walther Parson, Lutz Roewer, Bruce Budowle

Journal: ELECTROPHORESIS

Abstract The current state of validation and implementation strategies of massively parallel sequencing (MPS) technology for the analysis of STR markers for forensic genetics use is described, covering the topics of the current catalog of commercial MPS‐STR panels, leading MPS‐platforms, and MPS‐STR data analysis tools. In addition, the developmental and internal validation studies carried out to date to evaluate reliability,...

Read More thumbnail image: Current stateofart of STR sequencing in forensic genetics

Development of a massively parallel sequencing assay for investigating sequence polymorphisms of 15 short tandem repeats in a Chinese Northern Han population

Published: Jun 5, 2018

Author(s): Qing‐Xia Zhang, Meng Yang, Ya‐Jiao Pan, Jing Zhao, Bao‐Wang Qu, Feng Cheng, Ya‐Ran Yang, Zhang‐Ping Jiao, Li Liu, Jiang‐Wei Yan

Journal: ELECTROPHORESIS

Abstract Massively parallel sequencing (MPS) has been used in forensic genetics in recent years owing to several advantages, e.g. MPS can provide precise descriptions of the repeat allele structure and variation in the repeat‐flanking regions, increasing the discriminating power among loci and individuals. However, it cannot be fully utilized unless sufficient population data are available for all loci. Thus, there is a pressing...

Read More thumbnail image: Development of a massively parallel sequencing assay for investigating sequence polymorphisms of 15 short tandem repeats in a Chinese Northern Han population

Evaluation of massively parallel sequencing for forensic DNA methylation profiling

Published: Jun 5, 2018

Author(s): Rebecca Richards, Jayshree Patel, Kate Stevenson, SallyAnn Harbison

Journal: ELECTROPHORESIS

Abstract Epigenetics is an emerging area of interest in forensic science. DNA methylation, a type of epigenetic modification, can be applied to chronological age estimation, identical twin differentiation and body fluid identification. However, there is not yet an agreed, established methodology for targeted detection and analysis of DNA methylation markers in forensic research. Recently, a massively parallel sequencing‐based...

Read More thumbnail image: Evaluation of massively parallel sequencing for forensic DNA methylation profiling
Page:   Prev 6 7 8 9 10 11 12 13 14 15 16 Next

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2018 John Wiley & Sons, Inc. All Rights Reserved