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Development and validation of a sensitive LC‐MS/MS‐ESI method for the determination of ivabradine in human plasma: application to a pharmacokinetic study
Published: Jun 24, 2013
Author(s): Juanjuan Jiang, Lei Tian, Yiling Huang, Yishi Li
Journal: Biomedical Chromatography
ABSTRACT A sensitive, rapid assay method for estimating ivabradine in human plasma has been developed and validated using liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive‐ion mode. The procedure involved extraction of ivabradine and the internal standard (IS) from human plasma by solid‐phase extraction. Chromatographic separation was achieved using an isocratic mobile phase (0.1%...
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Pharmacokinetics of methyl salicylate‐2‐ O‐β ‐D‐lactoside, a novel salicylic acid analog isolated from Gaultheria yunnanensis ,in dogs
Published: Jun 24, 2013
Author(s): Dan Zhang, Xiaowei Ma, Wenyu Xin, Chao Huang, Weiku Zhang, Tiantai Zhang, Guanhua Du
Journal: Biomedical Chromatography
ABSTRACT Methyl salicylate‐2‐O‐β‐D‐lactoside (MSL), a natural salicylate derivative of Gaultheria yunnanensis (Franch.) Rehder (G. yunnanensis), has been shown to provide a beneficial anti‐inflammatory effect in animal models. Studies on the pharmacokinetics and bioavailability of MSL can provide both a substantial foundation for understanding its mechanism and empirical evidence to support its use in clinical practice. A simple and sensitive...
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Development and validation of automated SPE‐LC‐MS/MS method for determination of indapamide in human whole blood and its application to real study samples
Published: Jun 24, 2013
Author(s): N. Nakov, K. Mladenovska, N. Labacevski, A. Dimovski, R. Petkovska, A. Dimitrovska, Z. Kavrakovski
Journal: Biomedical Chromatography
ABSTRACT A fast and simple liquid chromatography–electrospray ionization tandem mass spectrometry method for determination of indapamide in human whole blood was developed and validated. The sample extraction of indapamide from human whole blood was achieved using automated solid‐phase extraction. Chromatographic separation was performed on Kinetex C18 column (100 × 2.1 mm, 1.7 µm particle size) using acetonitrile and 2 mm ammonium formate in...
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MS n , LC‐MS‐TOF and LC‐PDA studies for identification of new degradation impurities of bupropion
Published: Jun 24, 2013
Author(s): Rohit Bansal, Balraj Saini, Yogita Bansal, Gulshan Bansal
Journal: Biomedical Chromatography
ABSTRACT Three new degradation impurities of bupropion were characterized through high performance liquid chromatography coupled to photodiode array detection and to time‐of‐flight mass spectrometry. Bupropion was subjected to the ICH prescribed stress conditions. It degraded to seven impurities (I–VII) in alkaline hydrolytic conditions which were optimally resolved on an XTerra C18 column (250 × 4.6 mm, 5 µm) with a ternary mobile phase...
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Surface‐enhanced Raman spectra of the neonicotinoid pesticide thiacloprid
Published: Jun 23, 2013
Author(s): Maria Vega Cañamares, Alessandro Feis
Journal: Journal of Raman Spectroscopy
Thiacloprid is a widely used pesticide belonging to the neonicotinoid class, which is characterized by a selective activity against insects and a reduced acute toxicity for humans. The importance of the environmental impact of neonicotinoids is being intensively researched, in order to evaluate the danger they pose for useful insects. Physical methods which allow the characterization of neonicotinoids in diluted aqueous solutions are...
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Development and validation of a liquid chromatography–tandem mass spectrometry method for topotecan determination in beagle dog plasma and its application in a bioequivalence study
Published: Jun 21, 2013
Author(s): Ling Ye, Jian Shi, Shanhe Wan, Xiaoshan Yang, Ying Wang, Jiajie Zhang, Dayong Zheng, Zhongqiu Liu
Journal: Biomedical Chromatography
ABSTRACT Topotecan (TPT) is an important anti‐cancer drug that inhibits topoisomerase I. A sensitive and robust liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method that potentially determines TPT in beagle dog plasma is needed for a bioequivalence study of TPT formulations. We developed and validated LC‐MS/MS to evaluate TPT in beagle dog plasma in terms of specificity, linearity, precision, accuracy, stability,...
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Development and validation of an RP‐HPLC method for the quantitation of Orteronel (TAK‐700), a CYP17A1 enzyme inhibitor, in rat plasma and its application to a pharmacokinetic study
Published: Jun 21, 2013
Author(s): Avinash Kumar, S Vijay Kumar, Sandip Gurav, Mohd Zainuddin, Purushottam Dewang, Raghava Reddy Kethiri, Sriram Rajagopal, Ramesh Mullangi
Journal: Biomedical Chromatography
ABSTRACT A novel, simple, specific, sensitive and reproducible high‐performance liquid chromatography assay method has been developed and validated for the estimation of Orteronel in rat plasma. The bioanalytical procedure involves extraction of Orteronel and phenacetin (internal standard) from rat plasma with a simple liquid–liquid extraction process. The chromatographic analysis was performed on a Waters Alliance system using a...
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Timing dependence of peripheral pulse‐wave‐triggered pulsed arterial spin labeling
Published: Jun 20, 2013
Author(s): Yasutaka Fushimi, Tomohisa Okada, Akira Yamamoto, Mitsunori Kanagaki, Koji Fujimoto, Kaori Togashi
Journal: NMR in Biomedicine
Arterial spin labeling (ASL) has been developed into a useful technique that is capable of quantifying noninvasively local cerebral blood flow (CBF) using the water molecules in arterial blood as diffusible tracers. Pulsed ASL (PASL) is more strongly affected than continuous ASL (CASL) by cardiac pulsation, because the tag bolus is shorter than the cardiac cycle in most cases. No reports have yet clarified the effects of multiple cardiac...
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MR elastography in a murine stroke model reveals correlation of macroscopic viscoelastic properties of the brain with neuronal density
Published: Jun 20, 2013
Author(s): Florian Baptist Freimann, Susanne Müller, Kaspar‐Josche Streitberger, Jing Guo, Sergej Rot, Adnan Ghori, Peter Vajkoczy, Rolf Reiter, Ingolf Sack, Jürgen Braun
Journal: NMR in Biomedicine
The aim of this study was to investigate the influence of neuronal density on viscoelastic parameters of living brain tissue after ischemic infarction in the mouse using MR elastography (MRE). Transient middle cerebral artery occlusion (MCAO) in the left hemisphere was induced in 20 mice. In vivo 7‐T MRE at a vibration frequency of 900 Hz was performed on days 3, 7, 14 and 28 (n = 5 per group) after MCAO, followed by the analysis of histological...
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Improved three‐dimensional Look–Locker acquisition scheme and angle map filtering procedure for T 1 estimation
Published: Jun 20, 2013
Author(s): CheukKai Hui, Emilio Esparza‐Coss, Ponnada A. Narayana
Journal: NMR in Biomedicine
The three‐dimensional (3D) Look–Locker (LL) acquisition is a widely used fast and efficient T1 mapping method. However, the multi‐shot approach of 3D LL acquisition can introduce reconstruction artifacts that result in intensity distortions. Traditional 3D LL acquisition generally utilizes a centric encoding scheme that is limited to a single phase‐encoding direction in k space. To optimize k‐space segmentation, an elliptical scheme with two...
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Influence of the type of alkali metal cation on the interaction of soluble silicates with porous anodic alumina
Published: Jun 19, 2013
Author(s): R. Gaggiano, I. De Graeve, K. Verbeken, L. A. I. Kestens, H. Terryn
Journal: Surface and Interface Analysis
Aqueous sodium silicate solutions are commonly used in pretreatments of aluminium alloys as cleaners and corrosion inhibitors. Silicate films are a promising alternative for corrosion protection and are able to confer high hydrophilicity to the metal surface. An interaction mechanism of soluble sodium silicates with anodic alumina was proposed in previous publications, where the sodium cation acts as ‘bridging’ element between the...
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A comprehensive non‐invasive framework for automated evaluation of acute renal transplant rejection using DCE‐MRI
Published: Jun 18, 2013
Author(s): Fahmi Khalifa, Mohamed Abou El‐Ghar, Behnaz Abdollahi, Hermann B. Frieboes, Tarek El‐Diasty, Ayman El‐Baz
Journal: NMR in Biomedicine
The objective was to develop a novel and automated comprehensive framework for the non‐invasive identification and classification of kidney non‐rejection and acute rejection transplants using 2D dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI). The proposed approach consists of four steps. First, kidney objects are segmented from the surrounding structures with a geometric deformable model. Second, a...
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The impact of myelination on axon sparing and locomotor function recovery in spinal cord injury assessed using diffusion tensor imaging
Published: Jun 18, 2013
Author(s): Tsang‐Wei Tu, Joong H. Kim, Feng Qin Yin, Lyn B. Jakeman, Sheng‐Kwei Song
Journal: NMR in Biomedicine
The dysmyelinated axons of shiverer mice exhibit impaired conduction characteristics, similar to early postnatal axons before myelination, whereas the patterns of neuronal activity and connectivity are relatively comparable with those of wild‐type myelinated axons. This unique dysmyelination pattern is exploited in the present study to determine the role of compact myelin in the loss and recovery of function following traumatic spinal...
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Resonant inductive decoupling (RID) for transceiver arrays to compensate for both reactive and resistive components of the mutual impedance
Published: Jun 18, 2013
Author(s): Nikolai I. Avdievich, Jullie W. Pan, Hoby P. Hetherington
Journal: NMR in Biomedicine
Transceiver surface coil arrays improve transmit performance (B1/√kW) and B1 homogeneity for head imaging up to 9.4 T. To further improve reception performance and parallel imaging, the number of array elements must be increased with a corresponding decrease in their size. With a large number of small interacting antennas, decoupling is one of the most challenging aspects in the design and construction of transceiver arrays. Previously described ...
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Characterization of choline kinase in human endothelial cells
Published: Jun 18, 2013
Author(s): Noriko Mori, Mayur Gadiya, Flonné Wildes, Balaji Krishnamachary, Kristine Glunde, Zaver M. Bhujwalla
Journal: NMR in Biomedicine
High choline kinase‐α (Chk‐α) expression is frequently observed in cancer cells, making it a novel target for pharmacological and molecular inhibition. As inhibiting agents are delivered systemically, it is important to determine Chk‐α expression levels in endothelial cells that line both normal and tumor vasculature, and the effect of Chk‐α downregulation on these cells. Here, we characterized Chk‐α expression and the effect of its...
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Proteogenomics for environmental microbiology
Published: Jun 18, 2013
Author(s): Jean Armengaud, Erica Marie Hartmann, Céline Bland
Journal: PROTEOMICS
Proteogenomics sensu stricto refers to the use of proteomic data to refine the annotation of genomes from model organisms. Because of the limitations of automatic annotation pipelines, a relatively high number of errors occur during the structural annotation of genes coding for proteins. Whether putative orphan sequences or short genes encoding low‐molecular‐weight proteins really exist is still frequently a mystery. Whether start codons...
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The contribution of proteomics to the unveiling of the survival strategies used by P seudomonas putida in changing and hostile environments
Published: Jun 18, 2013
Author(s): Renata Moreno, Fernando Rojo
Journal: PROTEOMICS
Pseudomonas putida is a ubiquitous, metabolically very versatile, Gram‐negative bacterium adapted to habitats as diverse as soil, water and the rhizosphere. Most strains are nonpathogenic, many are used as experimental models, and many others have biotechnological applications in the areas of agriculture, bioremediation, biocatalysis, and the production of bioplastics. This review summarizes the contribution of proteomic technologies to...
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Elucidation of in situ polycyclic aromatic hydrocarbon degradation by functional metaproteomics (protein‐SIP)
Published: Jun 18, 2013
Author(s): Florian‐Alexander Herbst, Arne Bahr, Marcia Duarte, Dietmar H. Pieper, Hans‐Hermann Richnow, Martin Bergen, Jana Seifert, Petra Bombach
Journal: PROTEOMICS
Current knowledge of the physiology and phylogeny of polycyclic aromatic hydrocarbon (PAH) degrading bacteria often relies on laboratory enrichments and isolations. In the present study, in situ microcosms consisting of activated carbon pellets (BACTRAP®s) were loaded with either 13C‐naphthalene or 13C‐fluorene and were subsequently exposed in the contaminant source and plume fringe region of a PAH‐contaminated aquifer. Metaproteomic...
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NanoPad: An integrated platform for bacterial production of camel nanobodies aimed at detecting environmental biomarkers
Published: Jun 18, 2013
Author(s): Sofía Fraile, Jose I. Jiménez, Carlos Gutiérrez, Víctor Lorenzo
Journal: PROTEOMICS
The presence of given antigens in environmental samples (e.g. biodegradative enzymes) reports the quality and catalytic vigor of particular soils or aquatic ecosystems. In this context, we have developed the NanoPad system consisting of a complete platform for isolation, amplification, and extracellular production of specific antibodies against antigens that diagnose the occurrence of protein markers in crude environmental samples. The...
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Surfaceomics and surface‐enhanced R aman spectroscopy of environmental microbes: Matching cofactors with redox‐active surface proteins
Published: Jun 18, 2013
Author(s): Hans K. Carlson, Anthony T. Iavarone, John D. Coates
Journal: PROTEOMICS
Trypsin shaving is a targeted proteomic method for identifying cell‐surface exposed proteins on bacterial cells. For the identification of redox‐active cell‐surface proteins, trypsin‐shaving datasets can be matched with surface‐enhanced Raman spectra of intact cells to identify the cofactors associated with the cell‐surface proteins. Together, these approaches could help resolve questions about the presence of cell‐surface electron...
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