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Hydrophobic cryogels for DNA adsorption: Effect of embedding of monosize microbeads into cryogel network on their adsorptive performances

Published: Jun 18, 2013

Author(s): M. Emin Çorman, Nilay Bereli, Serpil Özkara, Lokman Uzun, Adil Denizli

Journal: Biomedical Chromatography

ABSTRACT As alternative hydrophobic adsorbent for DNA adsorption, supermacroporous cryogel disks were synthesized via free radical polymerization. In this study, we have prepared two kinds of cryogel disks: (i) poly(2‐hydroxyethyl methacrylate‐N‐methacryloyl‐l‐tryptophan) [p(HEMA‐MATrp)] cryogel containing specific hydrophobic ligand MATrp; and (ii) monosize p(HEMA‐MATrp) particles synthesized via suspension polymerization embedded into p(HEMA)...

Read More thumbnail image: Hydrophobic cryogels for DNA adsorption Effect of embedding of monosize microbeads into cryogel network on their adsorptive performances

Determination of flomoxef in human plasma by liquid chromatography/electrospray ionization tandem mass spectrometry

Published: Jun 18, 2013

Author(s): Oxana Yu. Kravtsova, Sergey A. Paramonov, Natalya I. Vasilevich, Denis N. Kazyulkin, Ekaterina Vlasova, Michael Engsig

Journal: Biomedical Chromatography

ABSTRACT A specific, sensitive, rapid and reproducible method for the determination of flomoxef in human plasma using high‐performance liquid chromatography–tandem mass spectrometry was developed and validated. Flomoxef was detected using an electrospay ionization method operated in negative‐ion mode. Chromatographic separation was performed in gradient elution mode on a Luna® C18(2) column (3 μm, 20 × 4.0 mm) at a flow rate of 1 mL/min and...

Read More thumbnail image: Determination of flomoxef in human plasma by liquid chromatographyelectrospray ionization tandem mass spectrometry

Rapid and simultaneous determination of multiple classes of abused drugs and metabolites in human urine by a robust LC‐MS/MS method – application to urine drug testing in pain clinics

Published: Jun 18, 2013

Author(s): Jianmei Wang, Zhen Yang, James Lechago

Journal: Biomedical Chromatography

ABSTRACT A simple LC‐MS/MS method was developed and validated for quantitatively analyzing six classes of 26 abused drugs and metabolites in human urine: (1) illicit drugs; (2) opiates; (3) synthetic opioids; (4) sedative; (5) stimulants; and (6) γ‐aminobutyric acid analogs. All urine samples were diluted with a mixture of isotope‐labeled internal standards, hydrolyzed with β‐glucuronidase and directly injected in a gradient chromatographic...

Read More thumbnail image: Rapid and simultaneous determination of multiple classes of abused drugs and metabolites in human urine by a robust LCMSMS method  application to urine drug testing in pain clinics

Fast analysis of triterpenoids in Ganoderma lucidum spores by ultra‐performance liquid chromatography coupled with triple quadrupole mass spectrometry

Published: Jun 18, 2013

Author(s): Zhou Yan, Bing Xia, Ming Hua Qiu, Ding Li Sheng, Hong Xi Xu

Journal: Biomedical Chromatography

ABSTRACT A rapid and reliable method was established for simultaneous determination of main triterpenoids in Ganoderma lucidum spores using ultra‐high‐performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC‐TQ‐MS). The established method was validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to determine the contents of 10 main triterpenoids in different...

Read More thumbnail image: Fast analysis of triterpenoids in Ganoderma lucidum spores by ultraperformance liquid chromatography coupled with triple quadrupole mass spectrometry

Application of antibiotics as chiral selectors for capillary electrophoretic enantioseparation of pharmaceuticals: a review

Published: Jun 18, 2013

Author(s): Shuchi Dixit, Jung Hag Park

Journal: Biomedical Chromatography

ABSTRACT Recent years have witnessed several new trends in chiral separation, for example, the enantiorecognition ability of several new antibiotics has been explored using capillary electrophoresis (CE) prior to HPLC; antibiotics have been employed as chiral selectors (CSs) in a nonaqueous CE (NACE) mode; and several new detection techniques (namely, capacitively coupled contactless conductivity detection) have been used in...

Read More thumbnail image: Application of antibiotics as chiral selectors for capillary electrophoretic enantioseparation of pharmaceuticals a review

A rapid and selective HPLC‐UV method for the quantitation of efavirenz in plasma from patients on concurrent HIV/AIDS and tuberculosis treatments

Published: Jun 18, 2013

Author(s): Emile Bienvenu, Kurt‐Jürgen Hoffmann, Michael Ashton, Pierre Claver Kayumba

Journal: Biomedical Chromatography

ABSTRACT Owing to heterogeneity in therapeutic response, efavirenz is of research and clinical interest. There is a need to quantitate it using noncostly and selective methods. A method for efavirenz quantitation in plasma containing HIV and tuberculosis drugs was developed. Chromatographic separation was carried out using a C18 column. The mobile phase consisted of 0.1% formic acid and acetonitrile, and was pumped at a flow rate of 0.3 mL/min....

Read More thumbnail image: A rapid and selective HPLCUV method for the quantitation of efavirenz in plasma from patients on concurrent HIVAIDS and tuberculosis treatments

Dynamic detection of non‐protein‐bound strychnine and brucine in rabbit muscle and synovial fluid after topical application of total Strychnos alkaloid patches

Published: Jun 17, 2013

Author(s): Huaibo Tang, Miao Yan, Huande Li, Tianrong Xun, Yang Deng, Yeye Zhao, Long Deng

Journal: Drug Testing and Analysis

Semen Strychni, a known toxic drug in Chinese pharmacopoeia, is notable for its therapeutic effects on local muscle and joint pain. However, oral administration can be risky. Topically administered drugs accumulate in the topical muscles and knee joints without any major increase in plasma levels; only non‐protein‐bound drugs in the biological fluids of target tissues are effective for therapeutic effects. A sensitive and rapid ultra...

Read More thumbnail image: Dynamic detection of nonproteinbound strychnine and brucine in rabbit muscle and synovial fluid after topical application of total Strychnos alkaloid patches

Raman spectroscopy: a tool for biomechanical characterization of Stratum Corneum

Published: Jun 15, 2013

Author(s): Raoul Vyumvuhore, Ali Tfayli, Hélène Duplan, Alexandre Delalleau, Michel Manfait, Arlette Baillet‐Guffroy

Journal: Journal of Raman Spectroscopy

The mechanical properties of the Stratum Corneum (SC) have been studied by different authors at the macroscopic level, but the modification of its ultra structure during mechanical extension remains unknown. Moreover, little is described about the effect of the mechanical stress on SC barrier function. In this study, we have examined the SC structure changes, at the molecular level, during uniaxial tensile experiments. This was performed...

Read More thumbnail image: Raman spectroscopy a tool for biomechanical characterization of Stratum Corneum

Determination of Zn distribution and speciation in basic oxygen furnace sludge by synchrotron radiation induced μ‐XRF and μ‐XANES microspectroscopy

Published: Jun 15, 2013

Author(s): Lihua Wang, Xiaoming Lu, Yuying Huang

Journal: X-Ray Spectrometry

Basic oxygen furnace off gas (BOF OG) sludge is one important type of metallurgical residues in steelmaking industry. The presence of zinc element is one key factor that constrains the practical use of such metallurgical residues. Information about the chemical association of zinc in BOF OG sludge is fundamental for understanding its behavior during the formation and further treatments. In this paper, the spatial distribution and the...

Read More thumbnail image: Determination of Zn distribution and speciation in basic oxygen furnace sludge by synchrotron radiation induced XRF and XANES microspectroscopy

Enantioselectivity of mass spectrometry: Challenges and promises

Published: Jun 15, 2013

Author(s): Hanan Awad, Anas El‐Aneed

Journal: Mass Spectrometry Reviews

Abstract With the fast growing market of pure enantiomer drugs and bioactive molecules, new chiral‐selective analytical tools have been instigated including the use of mass spectrometry (MS). Even though MS is one of the best analytical tools that has efficiently been used in several pharmaceutical and biological applications, traditionally MS is considered as a “chiral‐blind” technique. This limitation is due...

Read More thumbnail image: Enantioselectivity of mass spectrometry Challenges and promises

Getting intimate with trypsin, the leading protease in proteomics

Published: Jun 15, 2013

Author(s): Elien Vandermarliere, Michael Mueller, Lennart Martens

Journal: Mass Spectrometry Reviews

Abstract Nowadays, mass spectrometry‐based proteomics is carried out primarily in a bottom‐up fashion, with peptides obtained after proteolytic digest of a whole proteome lysate as the primary analytes instead of the proteins themselves. This experimental setup crucially relies on a protease to digest an abundant and complex protein mixture into a far more complex peptide mixture. Full knowledge of the working...

Read More thumbnail image: Getting intimate with trypsin the leading protease in proteomics

PROTEOMIC APPROACHES TO EVALUATE PROTEIN S ‐NITROSYLATION IN DISEASE

Published: Jun 15, 2013

Author(s): Laura M. López‐Sánchez, Chary López‐Pedrera, Antonio Rodríguez‐Ariza

Journal: Mass Spectrometry Reviews

Abstract Many of nitric oxide (NO) actions are mediated through the coupling of a nitroso moiety to a reactive cysteine leading to the formation of a S‐nitrosothiol (SNO), a process known as S‐nitrosylation or S‐nitrosation. In many cases this reversible post‐translational modification is accompanied by altered protein function and aberrant S‐nitrosylation of proteins, caused by altered production of NO and/or impaired SNO...

Read More thumbnail image: PROTEOMIC APPROACHES TO EVALUATE PROTEIN S NITROSYLATION IN DISEASE

Rapid synthesis and characterization of ultra‐thin shell Au@SiO 2 nanorods with tunable SPR for shell‐isolated nanoparticle‐enhanced Raman spectroscopy (SHINERS)

Published: Jun 14, 2013

Author(s): Bi‐Qi Zhang, Song‐Bo Li, Qing Xiao, Ju Li, Jian‐Jun Sun

Journal: Journal of Raman Spectroscopy

The recently reported shell‐isolated nanoparticle‐enhanced Raman spectroscopy (SHINERS) is considered as the next generation of advanced spectroscopy for its surface and molecular generality. With the aim to utilize the virtues of shell‐isolated strategy and advance the SHINERS technique, we introduce a silane‐based rapid synthesis method of silica‐coating Au nanorods (Au@SiO2 NRs) with manoeuvrable ultra‐thin shell and tunable SPR. The results...

Read More thumbnail image: Rapid synthesis and characterization of ultrathin shell AuSiO 2 nanorods with tunable SPR for shellisolated nanoparticleenhanced Raman spectroscopy SHINERS

Laser‐induced volume changes during confocal Raman microscopy of whey‐protein‐stabilized emulsions and their relationship to protein content and particle diameters

Published: Jun 14, 2013

Author(s): Marija Iljina, Stephen R. Euston, Henry T. Bookey

Journal: Journal of Raman Spectroscopy

The use of confocal Raman microscopy is applied to the study of whey‐protein‐stabilized oil‐in‐water emulsions. The stability of emulsions is an important parameter for many applications and is dependent on several factors including protein content and particle size. Emulsions were prepared with varying particle size ranging from 0.8 to 10.0 µm. Changes in sample volume due to laser‐induced heating were monitored and correlated to protein...

Read More thumbnail image: Laserinduced volume changes during confocal Raman microscopy of wheyproteinstabilized emulsions and their relationship to protein content and particle diameters

Metabolic imaging of acute and chronic infarction in the perfused rat heart using hyperpolarised [1‐ 13 C]pyruvate

Published: Jun 14, 2013

Author(s): Daniel R. Ball, Rachel Cruickshank, Carolyn A. Carr, Daniel J. Stuckey, Philip Lee, Kieran Clarke, Damian J. Tyler

Journal: NMR in Biomedicine

Hyperpolarised 13C MRI can be used to generate metabolic images of the heart in vivo. However, there have been no similar studies performed in the isolated perfused heart. Therefore, the aim of this study was to develop a method for the creation of 13C metabolite maps of the perfused rat heart and to demonstrate the technique in a study of acute and chronic myocardial infarction. Male Wistar rat hearts were isolated, perfused and imaged before and...

Read More thumbnail image: Metabolic imaging of acute and chronic infarction in the perfused rat heart using hyperpolarised 1 13 Cpyruvate

Ultrashort‐TE MRI longitudinal study and characterization of a chronic model of asthma in mice: inflammation and bronchial remodeling assessment

Published: Jun 13, 2013

Author(s): Andrea Bianchi, Annaïg Ozier, Olga Ousova, Gérard Raffard, Yannick Crémillieux

Journal: NMR in Biomedicine

Asthma is a chronic disease characterized by bronchial hyperresponsiveness (BHR), bronchial inflammation and remodeling. The great improvements in 1H MRI ultrashort‐TE (UTE) sequences in the last decade have allowed lung images with high‐resolution and good signal‐to‐noise ratio to be obtained in parenchymal tissues. In this article, we present a UTE 1H MRI high‐resolution study of a chronic model of asthma in mice with the aim to longitudinally...

Read More thumbnail image: UltrashortTE MRI longitudinal study and characterization of a chronic model of asthma in mice inflammation and bronchial remodeling assessment

Mapping of prostate cancer by 1 H MRSI

Published: Jun 13, 2013

Author(s): Thiele Kobus, Alan J. Wright, Tom W. J. Scheenen, Arend Heerschap

Journal: NMR in Biomedicine

In many studies, it has been demonstrated that 1H MRSI of the human prostate has great potential to aid prostate cancer management, e.g. in the detection and localisation of cancer foci in the prostate or in the assessment of its aggressiveness. It is particularly powerful in combination with T2‐weighted MRI. Nevertheless, the technique is currently mainly used in a research setting. This review provides an overview of the...

Read More thumbnail image: Mapping of prostate cancer by 1 H MRSI

Profiling and identification of the absorbed constituents and metabolites of schisandra lignans by ultra‐performance liquid chromatography coupled to mass spectrometry

Published: Jun 13, 2013

Author(s): Hui Sun, Fangfang Wu, Aihua Zhang, Wenfeng Wei, Ying Han, Xijun Wang

Journal: Biomedical Chromatography

ABSTRACT Schisandra chinensis Baill grows wild in Russia, China, Korea and Japan, and its fruit has been found to be effective in amnesia and insomnia. It is enriched in schisandra lignans (SL) that are major components responsible for therapeutic action. However, there are no reports on the biotransformation analysis of SL. An ultra‐performance liquid chromatography/electrospray‐ionization high‐definition mass spectrometry...

Read More thumbnail image: Profiling and identification of the absorbed constituents and metabolites of schisandra lignans by ultraperformance liquid chromatography coupled to mass spectrometry

A simple and accurate method to determine nitrite and nitrate in serum based on high‐performance liquid chromatography with fluorescence detection

Published: Jun 13, 2013

Author(s): Zhan Shu‐yu, Shao Qing, Liu Li, Fan Xiao‐hui

Journal: Biomedical Chromatography

ABSTRACT A simple method for accurate determination of nitrite and nitrate in serum was proposed to avoid the variation of nitrate reduction. For nitrite determination, serum samples were directly precipitated with methanol pre‐nitrate conversion, and then the supernatant reacted with 2,3‐diaminonaphthalene (DAN) to form 2,3‐naphthotriazole (NAT), which was quantitatively analyzed by high‐performance liquid chromatography coupled...

Read More thumbnail image: A simple and accurate method to determine nitrite and nitrate in serum based on highperformance liquid chromatography with fluorescence detection

An online coupled peritoneal macrophage/cell membrane chromatography and high‐performance liquid chromatography/mass spectrometry method to screen for anti‐inflammatory components from the Chinese traditional medicine Chloranthus multistachys Pei

Published: Jun 13, 2013

Author(s): Weifeng Li, Wei Xing, Sicen Wang, Ting Fan, Huimin Huang, Xiaofeng Niu, Langchong He

Journal: Biomedical Chromatography

ABSTRACT Cell membrane chromatography (CMC) is a chromatographic biological affinity method that uses specific cell membranes as the stationary phase. In this study, a novel peritoneal macrophage/cell membrane chromatography (PM/CMC)–online‐high performance liquid chromatography/mass spectrometry (HPLC/MS) method was established to screen for the anti‐inflammatory components from traditional Chinese medicines using hydrocortisone...

Read More thumbnail image: An online coupled peritoneal macrophagecell membrane chromatography and highperformance liquid chromatographymass spectrometry method to screen for antiinflammatory components from the Chinese traditional medicine Chloranthus multistachys Pei
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