EarlyView Articles
The very latest articles from the scientific literature
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Hydrophobic cryogels for DNA adsorption: Effect of embedding of monosize microbeads into cryogel network on their adsorptive performances
Published: Jun 18, 2013
Author(s): M. Emin Çorman, Nilay Bereli, Serpil Özkara, Lokman Uzun, Adil Denizli
Journal: Biomedical Chromatography
ABSTRACT As alternative hydrophobic adsorbent for DNA adsorption, supermacroporous cryogel disks were synthesized via free radical polymerization. In this study, we have prepared two kinds of cryogel disks: (i) poly(2‐hydroxyethyl methacrylate‐N‐methacryloyl‐l‐tryptophan) [p(HEMA‐MATrp)] cryogel containing specific hydrophobic ligand MATrp; and (ii) monosize p(HEMA‐MATrp) particles synthesized via suspension polymerization embedded into p(HEMA)...
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Determination of flomoxef in human plasma by liquid chromatography/electrospray ionization tandem mass spectrometry
Published: Jun 18, 2013
Author(s): Oxana Yu. Kravtsova, Sergey A. Paramonov, Natalya I. Vasilevich, Denis N. Kazyulkin, Ekaterina Vlasova, Michael Engsig
Journal: Biomedical Chromatography
ABSTRACT A specific, sensitive, rapid and reproducible method for the determination of flomoxef in human plasma using high‐performance liquid chromatography–tandem mass spectrometry was developed and validated. Flomoxef was detected using an electrospay ionization method operated in negative‐ion mode. Chromatographic separation was performed in gradient elution mode on a Luna® C18(2) column (3 μm, 20 × 4.0 mm) at a flow rate of 1 mL/min and...
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Rapid and simultaneous determination of multiple classes of abused drugs and metabolites in human urine by a robust LC‐MS/MS method – application to urine drug testing in pain clinics
Published: Jun 18, 2013
Author(s): Jianmei Wang, Zhen Yang, James Lechago
Journal: Biomedical Chromatography
ABSTRACT A simple LC‐MS/MS method was developed and validated for quantitatively analyzing six classes of 26 abused drugs and metabolites in human urine: (1) illicit drugs; (2) opiates; (3) synthetic opioids; (4) sedative; (5) stimulants; and (6) γ‐aminobutyric acid analogs. All urine samples were diluted with a mixture of isotope‐labeled internal standards, hydrolyzed with β‐glucuronidase and directly injected in a gradient chromatographic...
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Fast analysis of triterpenoids in Ganoderma lucidum spores by ultra‐performance liquid chromatography coupled with triple quadrupole mass spectrometry
Published: Jun 18, 2013
Author(s): Zhou Yan, Bing Xia, Ming Hua Qiu, Ding Li Sheng, Hong Xi Xu
Journal: Biomedical Chromatography
ABSTRACT A rapid and reliable method was established for simultaneous determination of main triterpenoids in Ganoderma lucidum spores using ultra‐high‐performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC‐TQ‐MS). The established method was validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to determine the contents of 10 main triterpenoids in different...
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Application of antibiotics as chiral selectors for capillary electrophoretic enantioseparation of pharmaceuticals: a review
Published: Jun 18, 2013
Author(s): Shuchi Dixit, Jung Hag Park
Journal: Biomedical Chromatography
ABSTRACT Recent years have witnessed several new trends in chiral separation, for example, the enantiorecognition ability of several new antibiotics has been explored using capillary electrophoresis (CE) prior to HPLC; antibiotics have been employed as chiral selectors (CSs) in a nonaqueous CE (NACE) mode; and several new detection techniques (namely, capacitively coupled contactless conductivity detection) have been used in...
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A rapid and selective HPLC‐UV method for the quantitation of efavirenz in plasma from patients on concurrent HIV/AIDS and tuberculosis treatments
Published: Jun 18, 2013
Author(s): Emile Bienvenu, Kurt‐Jürgen Hoffmann, Michael Ashton, Pierre Claver Kayumba
Journal: Biomedical Chromatography
ABSTRACT Owing to heterogeneity in therapeutic response, efavirenz is of research and clinical interest. There is a need to quantitate it using noncostly and selective methods. A method for efavirenz quantitation in plasma containing HIV and tuberculosis drugs was developed. Chromatographic separation was carried out using a C18 column. The mobile phase consisted of 0.1% formic acid and acetonitrile, and was pumped at a flow rate of 0.3 mL/min....
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Dynamic detection of non‐protein‐bound strychnine and brucine in rabbit muscle and synovial fluid after topical application of total Strychnos alkaloid patches
Published: Jun 17, 2013
Author(s): Huaibo Tang, Miao Yan, Huande Li, Tianrong Xun, Yang Deng, Yeye Zhao, Long Deng
Journal: Drug Testing and Analysis
Semen Strychni, a known toxic drug in Chinese pharmacopoeia, is notable for its therapeutic effects on local muscle and joint pain. However, oral administration can be risky. Topically administered drugs accumulate in the topical muscles and knee joints without any major increase in plasma levels; only non‐protein‐bound drugs in the biological fluids of target tissues are effective for therapeutic effects. A sensitive and rapid ultra...
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Raman spectroscopy: a tool for biomechanical characterization of Stratum Corneum
Published: Jun 15, 2013
Author(s): Raoul Vyumvuhore, Ali Tfayli, Hélène Duplan, Alexandre Delalleau, Michel Manfait, Arlette Baillet‐Guffroy
Journal: Journal of Raman Spectroscopy
The mechanical properties of the Stratum Corneum (SC) have been studied by different authors at the macroscopic level, but the modification of its ultra structure during mechanical extension remains unknown. Moreover, little is described about the effect of the mechanical stress on SC barrier function. In this study, we have examined the SC structure changes, at the molecular level, during uniaxial tensile experiments. This was performed...
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Determination of Zn distribution and speciation in basic oxygen furnace sludge by synchrotron radiation induced μ‐XRF and μ‐XANES microspectroscopy
Published: Jun 15, 2013
Author(s): Lihua Wang, Xiaoming Lu, Yuying Huang
Journal: X-Ray Spectrometry
Basic oxygen furnace off gas (BOF OG) sludge is one important type of metallurgical residues in steelmaking industry. The presence of zinc element is one key factor that constrains the practical use of such metallurgical residues. Information about the chemical association of zinc in BOF OG sludge is fundamental for understanding its behavior during the formation and further treatments. In this paper, the spatial distribution and the...
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Enantioselectivity of mass spectrometry: Challenges and promises
Published: Jun 15, 2013
Author(s): Hanan Awad, Anas El‐Aneed
Journal: Mass Spectrometry Reviews
Abstract With the fast growing market of pure enantiomer drugs and bioactive molecules, new chiral‐selective analytical tools have been instigated including the use of mass spectrometry (MS). Even though MS is one of the best analytical tools that has efficiently been used in several pharmaceutical and biological applications, traditionally MS is considered as a “chiral‐blind” technique. This limitation is due...
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Getting intimate with trypsin, the leading protease in proteomics
Published: Jun 15, 2013
Author(s): Elien Vandermarliere, Michael Mueller, Lennart Martens
Journal: Mass Spectrometry Reviews
Abstract Nowadays, mass spectrometry‐based proteomics is carried out primarily in a bottom‐up fashion, with peptides obtained after proteolytic digest of a whole proteome lysate as the primary analytes instead of the proteins themselves. This experimental setup crucially relies on a protease to digest an abundant and complex protein mixture into a far more complex peptide mixture. Full knowledge of the working...
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PROTEOMIC APPROACHES TO EVALUATE PROTEIN S ‐NITROSYLATION IN DISEASE
Published: Jun 15, 2013
Author(s): Laura M. López‐Sánchez, Chary López‐Pedrera, Antonio Rodríguez‐Ariza
Journal: Mass Spectrometry Reviews
Abstract Many of nitric oxide (NO) actions are mediated through the coupling of a nitroso moiety to a reactive cysteine leading to the formation of a S‐nitrosothiol (SNO), a process known as S‐nitrosylation or S‐nitrosation. In many cases this reversible post‐translational modification is accompanied by altered protein function and aberrant S‐nitrosylation of proteins, caused by altered production of NO and/or impaired SNO...
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Rapid synthesis and characterization of ultra‐thin shell Au@SiO 2 nanorods with tunable SPR for shell‐isolated nanoparticle‐enhanced Raman spectroscopy (SHINERS)
Published: Jun 14, 2013
Author(s): Bi‐Qi Zhang, Song‐Bo Li, Qing Xiao, Ju Li, Jian‐Jun Sun
Journal: Journal of Raman Spectroscopy
The recently reported shell‐isolated nanoparticle‐enhanced Raman spectroscopy (SHINERS) is considered as the next generation of advanced spectroscopy for its surface and molecular generality. With the aim to utilize the virtues of shell‐isolated strategy and advance the SHINERS technique, we introduce a silane‐based rapid synthesis method of silica‐coating Au nanorods (Au@SiO2 NRs) with manoeuvrable ultra‐thin shell and tunable SPR. The results...
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Laser‐induced volume changes during confocal Raman microscopy of whey‐protein‐stabilized emulsions and their relationship to protein content and particle diameters
Published: Jun 14, 2013
Author(s): Marija Iljina, Stephen R. Euston, Henry T. Bookey
Journal: Journal of Raman Spectroscopy
The use of confocal Raman microscopy is applied to the study of whey‐protein‐stabilized oil‐in‐water emulsions. The stability of emulsions is an important parameter for many applications and is dependent on several factors including protein content and particle size. Emulsions were prepared with varying particle size ranging from 0.8 to 10.0 µm. Changes in sample volume due to laser‐induced heating were monitored and correlated to protein...
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Metabolic imaging of acute and chronic infarction in the perfused rat heart using hyperpolarised [1‐ 13 C]pyruvate
Published: Jun 14, 2013
Author(s): Daniel R. Ball, Rachel Cruickshank, Carolyn A. Carr, Daniel J. Stuckey, Philip Lee, Kieran Clarke, Damian J. Tyler
Journal: NMR in Biomedicine
Hyperpolarised 13C MRI can be used to generate metabolic images of the heart in vivo. However, there have been no similar studies performed in the isolated perfused heart. Therefore, the aim of this study was to develop a method for the creation of 13C metabolite maps of the perfused rat heart and to demonstrate the technique in a study of acute and chronic myocardial infarction. Male Wistar rat hearts were isolated, perfused and imaged before and...
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Ultrashort‐TE MRI longitudinal study and characterization of a chronic model of asthma in mice: inflammation and bronchial remodeling assessment
Published: Jun 13, 2013
Author(s): Andrea Bianchi, Annaïg Ozier, Olga Ousova, Gérard Raffard, Yannick Crémillieux
Journal: NMR in Biomedicine
Asthma is a chronic disease characterized by bronchial hyperresponsiveness (BHR), bronchial inflammation and remodeling. The great improvements in 1H MRI ultrashort‐TE (UTE) sequences in the last decade have allowed lung images with high‐resolution and good signal‐to‐noise ratio to be obtained in parenchymal tissues. In this article, we present a UTE 1H MRI high‐resolution study of a chronic model of asthma in mice with the aim to longitudinally...
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Mapping of prostate cancer by 1 H MRSI
Published: Jun 13, 2013
Author(s): Thiele Kobus, Alan J. Wright, Tom W. J. Scheenen, Arend Heerschap
Journal: NMR in Biomedicine
In many studies, it has been demonstrated that 1H MRSI of the human prostate has great potential to aid prostate cancer management, e.g. in the detection and localisation of cancer foci in the prostate or in the assessment of its aggressiveness. It is particularly powerful in combination with T2‐weighted MRI. Nevertheless, the technique is currently mainly used in a research setting. This review provides an overview of the...
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Profiling and identification of the absorbed constituents and metabolites of schisandra lignans by ultra‐performance liquid chromatography coupled to mass spectrometry
Published: Jun 13, 2013
Author(s): Hui Sun, Fangfang Wu, Aihua Zhang, Wenfeng Wei, Ying Han, Xijun Wang
Journal: Biomedical Chromatography
ABSTRACT Schisandra chinensis Baill grows wild in Russia, China, Korea and Japan, and its fruit has been found to be effective in amnesia and insomnia. It is enriched in schisandra lignans (SL) that are major components responsible for therapeutic action. However, there are no reports on the biotransformation analysis of SL. An ultra‐performance liquid chromatography/electrospray‐ionization high‐definition mass spectrometry...
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A simple and accurate method to determine nitrite and nitrate in serum based on high‐performance liquid chromatography with fluorescence detection
Published: Jun 13, 2013
Author(s): Zhan Shu‐yu, Shao Qing, Liu Li, Fan Xiao‐hui
Journal: Biomedical Chromatography
ABSTRACT A simple method for accurate determination of nitrite and nitrate in serum was proposed to avoid the variation of nitrate reduction. For nitrite determination, serum samples were directly precipitated with methanol pre‐nitrate conversion, and then the supernatant reacted with 2,3‐diaminonaphthalene (DAN) to form 2,3‐naphthotriazole (NAT), which was quantitatively analyzed by high‐performance liquid chromatography coupled...
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An online coupled peritoneal macrophage/cell membrane chromatography and high‐performance liquid chromatography/mass spectrometry method to screen for anti‐inflammatory components from the Chinese traditional medicine Chloranthus multistachys Pei
Published: Jun 13, 2013
Author(s): Weifeng Li, Wei Xing, Sicen Wang, Ting Fan, Huimin Huang, Xiaofeng Niu, Langchong He
Journal: Biomedical Chromatography
ABSTRACT Cell membrane chromatography (CMC) is a chromatographic biological affinity method that uses specific cell membranes as the stationary phase. In this study, a novel peritoneal macrophage/cell membrane chromatography (PM/CMC)–online‐high performance liquid chromatography/mass spectrometry (HPLC/MS) method was established to screen for the anti‐inflammatory components from traditional Chinese medicines using hydrocortisone...
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